Figure 1Effect of differential fractions extracted from the seeds of Bunium persicum on different hematological parameters compared from CBC results and free radical concentration in serum. Groups A, B, E, and H were administered intraperitoneally (i.p.) with nontoxic dose of 300 mg/kg of rat body weight of each fraction in 5% Tween solution for 9 consecutive days. Groups C and D were assigned as vehicle controls and were only injected (i.p.) with 0.5 mL/d of 5% Tween solution. Mean values were compared using one-way ANOVA followed by Tukey test. *A p value < 0.05 indicates significant difference from the control disease (group D) values. (A) Percentage of basophils in different groups. (B) % of eosinophils. (C) % of neutrophils. (D) Numbers of platelets. (E) RBC counts. (F) % of monocytes. (G) Level of ROS in different groups. ANOVA = analysis of variance; CBC = complete blood count; RBC = red blood cell; ROS = reactive oxygen species.
Figure 2Bone marrow smear stained with Giemsa stain. The blast cells are indicated by an arrow in each figure. (A) Control disease. (B) normal. (C) Blast cell in EFT. (D) Blast cells in BFT. BFT = .
Table 1Treatment schedule.
Group |
Treatment |
A |
Injected with leukemic blood; treated with aqueous fraction |
B |
Injected with leukemic blood; treated with butanol fraction |
C |
Designated as control group (normal); treated with 0.5 mL of 5% Tween |
D |
Assigned as disease control group; injected with leukemic blood and treated with 0.5 mL of 5% Tween |
E |
Injected with leukemic blood; treated with ethyl acetate fraction |
H |
Injected with leukemic blood; treated with hexane fraction |
Table 2Variation of differential blood parameters and comparative analysis of ROS mean values among normal control, disease control, and treated groups.
Groups |
WBC differential
|
RBC (g/dL) |
Platelet counts (×109/L) |
% Monocytes |
Basophils % |
Eosinophils % |
Neutrophils |
A |
29.7 ± 1.25 |
6.48 ± 0.91 |
5.75 ± 0.5 |
6.69 ± 0.76 |
5.70 ± 0.76 |
716 ± 28 |
B |
5.4 ± 0.7 |
4.95 ± 0.88 |
5.69 ± 0.86 |
7.47 ± 0.91 |
5.68 ± 0.52 |
102 ± 109 |
C |
6.57 ± 0.40 |
1.0 ± 0.50 |
5.18 ± 1.1 |
13.63 ± 0.9 |
7.6 ± 0.86 |
851 ± 89 |
D |
31.1 ± 0.90 |
6.87 ± 1.51 |
9.43 ± 0.7 |
6.10 ± 0.30 |
4.84 ± 0.76 |
411 ± 44 |
E |
11.2 ± 1.71 |
4.3 ± 1.02 |
5.2 ± 0.36 |
9.77 ± 0.3 |
6.48 ± 0.37 |
739 ± 50 |
H |
30.1 ± 0.76 |
6.48 ± 0.91 |
5.38 ± 0.58 |
6.99 ± 0.6 |
5.35 ± 0.22 |
538 ± 62 |
|
Mean value of absorbance (ROS) |
A |
0.24 ± 0.025 |
|
B |
0.25 ± 0.007 |
|
C |
0.21 ± 0.083 |
|
D |
0.41 ± 0.067 |
|
E |
0.24 ± 0.025 |
|
H |
0.35 ± 0.029 |
|