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Byung-Suk Chung 1 Article
Development of a Diagnostic Kit to Detect Cryptosporidium parvum and Giardia lamblia
Hyeng-Il Cheun, Byung-Suk Chung, Da-Won Ma, Bo-La Goo, Shin-Hyeong Cho, Mi-jung Ji, Won-Ja Lee
Osong Public Health Res Perspect. 2013;4(3):146-151.   Published online June 30, 2013
DOI: https://doi.org/10.1016/j.phrp.2013.04.006
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  • 18 Download
  • 7 Citations
AbstractAbstract PDF
Objectives
This study aims to develop a high-sensitivity antibody diagnostic kit that will enable a rapid and accurate detection of Cryptospofidium parvum and Giardia lamblia in patients with diarrhea.
Methods
The cultivated C. parvum oocysts and G. lamblia cysts in each calf and dog were injected to mice to obtain antibodies, which were titrated. Spleen cells of the immunized mouse were separated and blended with myelomas to produce hybrid cell lines that form monoclonal antibodies. Using ELISA method, antibodies that specifically respond to C. parvum and G.lamblia were then selected. The cells were injected into the abdominal cavity of a BALB/c mouse to isolate hydrops abdominis containing high level of antibodies. The IgG antibody was purified using protein G gel.
Results
The detection limit of monoclonal antibodies for Cryptosporidium parvum and Giardia lamblia was 125 oocysts/mL and 1250 cysts/mL, respectively. In addition, during testing they did not show cross-reactivity to viruses (n = 15), bacteria (n =17), and parasites (n = 9).
Conclusion
The rapid diagnostic antibody kit developed in this study, which specifically responds to C. parvum and G. lamblia, will be useful in detecting and monitoring diarrheal infections.

Citations

Citations to this article as recorded by  
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    George Luka, Ehsan Samiei, Nishat Tasnim, Arash Dalili, Homayoun Najjaran, Mina Hoorfar
    Journal of Hazardous Materials.2022; 421: 126714.     CrossRef
  • A review ofCryptosporidiumspp. and their detection in water
    Eman M. Hassan, Banu Örmeci, Maria C. DeRosa, Brent R. Dixon, Syed A. Sattar, Asma Iqbal
    Water Science and Technology.2021; 83(1): 1.     CrossRef
  • Portable on-chip colorimetric biosensing platform integrated with a smartphone for label/PCR-free detection of Cryptosporidium RNA
    George S. Luka, Ephraim Nowak, Quin Robert Toyata, Nishat Tasnim, Homayoun Najjaran, Mina Hoorfar
    Scientific Reports.2021;[Epub]     CrossRef
  • Nanomaterials-based biosensors for detection of microorganisms and microbial toxins
    Laura Sutarlie, Sian Yang Ow, Xiaodi Su
    Biotechnology Journal.2017;[Epub]     CrossRef
  • ACG Clinical Guideline: Diagnosis, Treatment, and Prevention of Acute Diarrheal Infections in Adults
    Mark S Riddle, Herbert L DuPont, Bradley A Connor
    American Journal of Gastroenterology.2016; 111(5): 602.     CrossRef
  • Multiplex-Touchdown PCR to Simultaneously Detect Cryptosporidium parvum, Giardia lamblia, and Cyclospora cayetanensis, the Major Causes of Traveler’s Diarrhea
    Ji-Hun Shin, Sang-Eun Lee, Tong Soo Kim, Da-Won Ma, Jong-Yil Chai, Eun-Hee Shin
    The Korean Journal of Parasitology.2016; 54(5): 631.     CrossRef
  • Advancing Cryptosporidium Diagnostics from Bench to Bedside
    Raul V. Destura, Rohani B. Cena, Ma. Jowina H. Galarion, Coleen M. Pangilinan, Geraldine M. Arevalo, Ryan Oliver C. Alba, Joy Ann G. Petronio, Gielenny M. Salem, Brian Schwem, Jesus Emmanuel A. D. Sevilleja
    Current Tropical Medicine Reports.2015; 2(3): 150.     CrossRef

PHRP : Osong Public Health and Research Perspectives