Skip Navigation
Skip to contents

PHRP : Osong Public Health and Research Perspectives


Author index

Page Path
HOME > Articles and issues > Author index
Chunhwa Ihm 1 Article
Rapid DNA Extraction from Dried Blood Spots on Filter Paper: Potential Applications in Biobanking
Eun-Hye Choi, Sang Kwang Lee, Chunhwa Ihm, Young-Hak Sohn
Osong Public Health Res Perspect. 2014;5(6):351-357.   Published online December 31, 2014
  • 3,360 View
  • 35 Download
  • 42 Crossref
AbstractAbstract PDF
Dried blood spot (DBS) technology is a microsampling alternative to traditional plasma or serum sampling for pharmaco- or toxicokinetic evaluation. DBS technology has been applied to diagnostic screening in drug discovery, nonclinical, and clinical settings. We have developed an improved elution protocol involving boiling of blood spots dried on Whatman filter paper.
The purpose of this study was to compare the quality, purity, and quantity of DNA isolated from frozen blood samples and DBSs. We optimized a method for extraction and estimation of DNA from blood spots dried on filter paper (3-mm FTA card). A single DBS containing 40 μL blood was used.
DNA was efficiently extracted in phosphate-buffered saline (PBS) or Tris-EDTA (TE) buffer by incubation at 37°C overnight. DNA was stable in DBSs that were stored at room temperature or frozen. The housekeeping genes GAPDH and beta-actin were used as positive standards for polymerase chain reaction (PCR) validation of general diagnostic screening.
Our simple and convenient DBS storage and extraction methods are suitable for diagnostic screening by using very small volumes of blood collected on filter paper, and can be used in biobanks for blood sample storage.


Citations to this article as recorded by  
  • Insight into Increased Recovery and Simplification of Genomic DNA Extraction Methods from Dried Blood Spots
    Kiara Lee, Anubhav Tripathi
    Biopreservation and Biobanking.2024; 22(2): 130.     CrossRef
  • Haptoglobin gene polymorphism and iron profile in sickle cell disease patients with inflammation in Yaounde, Cameroon
    Romaric De Manfouo Tuono, Josué Louokdom Simo, Prosper Cabral Biapa Nya, Jean Paul Chedjou, Christian Bernard Kengne Fotsing, Bernard Claude Chetcha, Calvino Fomboh Tah, Claude Tagny Tayou, Wilfried Fon Mbatcham, Constant Anatole Pieme
    Molecular Genetics & Genomic Medicine.2024;[Epub]     CrossRef
  • Detection of Duffy blood group genotypes and submicroscopic Plasmodium infections using molecular diagnostic assays in febrile malaria patients
    Beka R. Abagero, Rei Rama, Abdulghani Obeid, Tirusew Tolosa, Biniyam Lukas, Taye Teka, Daniel Tesfaye, Eugenia Lo, Delenasaw Yewhalaw
    Malaria Journal.2024;[Epub]     CrossRef
  • CRISPR-Based Diagnostics: Challenges and Potential Solutions toward Point-of-Care Applications
    Ahmed Ghouneimy, Ahmed Mahas, Tin Marsic, Rashid Aman, Magdy Mahfouz
    ACS Synthetic Biology.2023; 12(1): 1.     CrossRef
  • Effect of storage temperature and duration on direct PCR amplification of various feather types and DBS matrices
    Maryam Aslam, Fatima Naeem, Rijaab Seher, Muhammad Zubair Shabbir, Wasim Shehzad, Muhammad Imran
    Gene.2023; 854: 147116.     CrossRef
  • Improvement of bovine pestiviral diagnosis by the development of a cost-effective method for detecting viral RNA in fresh specimens and samples spotted in filter papers
    Paula M. Favaro, Ana Molineri, Maria J. Dus Santos, Luis F. Calvinho, Andrea Pecora
    Revista Argentina de Microbiología.2023; 55(2): 167.     CrossRef
  • A dual paper-based nucleic acid extraction method from blood in under ten minutes for point-of-care diagnostics
    Kenny Malpartida-Cardenas, Jake Baum, Aubrey Cunnington, Pantelis Georgiou, Jesus Rodriguez-Manzano
    The Analyst.2023; 148(13): 3036.     CrossRef
  • Current Understanding of Dried Spots Platform for Blood Proteomics
    Luis Perpétuo, Hugo Rocha, Rita Ferreira, Bruno Manadas, Francisco Amado, Sofia Guedes, Atef Mahmoud Mannaa, Jerome Vialaret, Christophe Hirtz, Rui Vitorino
    Current Proteomics.2023; 20(2): 81.     CrossRef
  • Comparative analysis of reagent kits for DNA extraction from dry blood stains
    A. V. Sedykh, M. A. Saitgalina, Yu. V. Ostankova, A. A. Totolian
    Medical Immunology (Russia).2023; 25(6): 1453.     CrossRef
  • Molecular Detection Challenges of Human Plasmodium Knowlesi Infection by Polymerase Chain Reaction and Sequencing
    Erma Sulistyaningsih, Resy Metri Belizani, Irawan Fajar Kusuma, Sahrir Sillehu, Rosita Dewi
    Journal of Biomedicine and Translational Research.2023; 9(2): 55.     CrossRef
  • Large-scale Extraction of DNA by Using Salting-out Principle for Dried Blood Spots to Screen Multiple Mutations in GCDH Gene
    Muntaj Shaik, A. Alladi, AB. Vedamurthy, KS. Devaraju, M. Kamate, TP. Kruthika-Vinod
    Iranian Journal of Science and Technology, Transac.2022; 46(1): 33.     CrossRef
  • Towards Next-Generation Sequencing (NGS)-Based Newborn Screening: A Technical Study to Prepare for the Challenges Ahead
    Abigail Veldman, Mensiena B. G. Kiewiet, Margaretha Rebecca Heiner-Fokkema, Marcel R. Nelen, Richard J. Sinke, Birgit Sikkema-Raddatz, Els Voorhoeve, Dineke Westra, Martijn E. T. Dollé, Peter C. J. I. Schielen, Francjan J. van Spronsen
    International Journal of Neonatal Screening.2022; 8(1): 17.     CrossRef
  • Upregulated NOTCH Signaling in the Lens of Patients With Pseudoexfoliation Syndrome Compared With Pseudoexfoliation Glaucoma Suggests Protective Role
    Zia S. Pradhan, Shivani Dixit, Lekshmi Krishna, Reshma Shetty, Sushma Tejwani, Harsha L. Rao, Rohit Shetty, Carroll A.B. Webers, Debashish Das
    Journal of Glaucoma.2022; 31(3): e1.     CrossRef
  • Electro-DBS: A Simple Method to Rapidly Extract Genomic DNA from Dried Blood Spots
    Kiara Lee, John Murphy, Anubhav Tripathi
    Analytical Chemistry.2022; 94(39): 13404.     CrossRef
  • Population genomics of a predatory mammal reveals patterns of decline and impacts of exposure to toxic toads
    Brenton von Takach, Louis Ranjard, Christopher P. Burridge, Skye F. Cameron, Teigan Cremona, Mark D. B. Eldridge, Diana O. Fisher, Stephen Frankenberg, Brydie M. Hill, Rosemary Hohnen, Chris J. Jolly, Ella Kelly, Anna J. MacDonald, Adnan Moussalli, Kym Ot
    Molecular Ecology.2022; 31(21): 5468.     CrossRef
  • A handmade DNA extraction kit using laundry powder; insights on simplicity, cost-efficiency, rapidity, safety and the quality of purified DNA
    Reza Talebi, Ramin Seighalani, Saber Qanbari
    Animal Biotechnology.2021; 32(3): 388.     CrossRef
  • Molecular Investigation on Tick-Borne Hemoparasites and Coxiella burnetii in Dromedary Camels (Camelusdromedarius) in Al Dhafra Region of Abu Dhabi, UAE
    El Tigani Ahmed El Tigani-Asil, Valeria Blanda, Ghada Elderdiri Abdelwahab, Zulaikha Mohamed Al Hammadi, Shameem Habeeba, Abdelmalik Ibrahim Khalafalla, Mohamed Ali Alhosani, Francesco La Russa, Sergio Migliore, Alessandra Torina, Guido Ruggero Loria, Sal
    Animals.2021; 11(3): 666.     CrossRef
  • Detection of Schistosoma mansoni DNA using polymerase chain reaction from serum and dried blood spot card samples of an adult population in North-western Tanzania
    Antje Fuss, Humphrey D. Mazigo, Andreas Mueller
    Infectious Diseases of Poverty.2021;[Epub]     CrossRef
  • Mind the Quality Gap When Banking on Dry Blood Spots
    David Carpentieri, Amber Colvard, Jackie Petersen, William Marsh, Victoria David-Dirgo, Matt Huentelman, Patrick Pirrotte, T.A. Sivakumaran
    Biopreservation and Biobanking.2021; 19(2): 136.     CrossRef
  • Long-Term Preservation and Storage of Faecal Samples in Whatman® Cards for PCR Detection and Genotyping of Giardia duodenalis and Cryptosporidium hominis
    Pamela Carolina Köster, Begoña Bailo, Alejandro Dashti, Carolina Hernández-Castro, Rafael Calero-Bernal, Francisco Ponce-Gordo, David González-Barrio, David Carmena
    Animals.2021; 11(5): 1369.     CrossRef
  • Preserved Blood Spots Aid Antenatal Diagnosis of Citrullinemia Type-1
    Shruti Bajaj, Uday Joglekar, Anil Jalan, Johannes Häberle, Veronique Rüfenacht
    Journal of Fetal Medicine.2021; 08(02): 157.     CrossRef
  • The genome of the zoonotic malaria parasite Plasmodium simium reveals adaptations to host switching
    Tobias Mourier, Denise Anete Madureira de Alvarenga, Abhinav Kaushik, Anielle de Pina-Costa, Olga Douvropoulou, Qingtian Guan, Francisco J. Guzmán-Vega, Sarah Forrester, Filipe Vieira Santos de Abreu, Cesare Bianco Júnior, Julio Cesar de Souza Junior, Sil
    BMC Biology.2021;[Epub]     CrossRef
  • Utilization of archived neonatal dried blood spots for genome-wide genotyping
    Pagna Sok, Philip J. Lupo, Melissa A. Richard, Karen R. Rabin, Erik A. Ehli, Noah A. Kallsen, Gareth E. Davies, Michael E. Scheurer, Austin L. Brown, Isabelle Chemin
    PLOS ONE.2020; 15(2): e0229352.     CrossRef
  • Ethnicity, age and disease-associated variation in body fluid-specific CpG sites in a diverse South African cohort
    Farzeen Kader, Meenu Ghai, Marvellous Zhou
    Forensic Science International.2020; 314: 110372.     CrossRef
  • Gene Doping and Genomic Science in Sports: Where Are We?
    Sheila López, João Meirelles, Vanessa Rayol, Gabriella Poralla, Nicole Woldmar, Bruna Fadel, Mariana Figueiredo, Mônica da Costa Padilha, Francisco Radler de Aquino Neto, Henrique Marcelo Gualberto Pereira, Luciana Pizzatti
    Bioanalysis.2020; 12(11): 801.     CrossRef
  • Development of novel extraction reagents for analyzing dried blood spots from crime scenes
    Hae-Min Lee, Jung-Hyeon Yang, Sun-Yeong Gwon, Hee-Gyoo Kang, Sung Hee Hyun, Jiyeong Lee, Ho Joong Sung
    Forensic Science International.2020; 317: 110531.     CrossRef
  • Development of a Multiplex Loop-Mediated Isothermal Amplification (LAMP) Method for Simultaneous Detection of Spotted Fever Group Rickettsiae and Malaria Parasites by Dipstick DNA Chromatography
    Lavel Chinyama Moonga, Kyoko Hayashida, Naoko Kawai, Ryo Nakao, Chihiro Sugimoto, Boniface Namangala, Junya Yamagishi
    Diagnostics.2020; 10(11): 897.     CrossRef
  • DNA Adsorption Studies of Poly(4,4′-Cychlohexylidene Bisphenol Oxalate)/Silica Nanocomposites
    Aisha Nawaf Al balawi, Nor Azah Yusof, Sazlinda Kamaruzaman, Faruq Mohammad, Helmi Wasoh, Hamad A. Al-Lohedan
    Materials.2019; 12(7): 1178.     CrossRef
  • Evaluation of commercial methods to separate nucleic acids from intestinal tissues of pigs for diagnosis of porcine epidemic diarrhea
    D. М. Masiuk, V. S. Nedzvetsky, A. V. Kokariev, O. V. Danchuk, T. O. Vasilenko, O. M. Yefimova
    Regulatory Mechanisms in Biosystems.2019; 10(4): 477.     CrossRef
  • Comparative analysis of three methods from dried blood spots for expeditious DNA extraction from mosquitoes; suitable for PCR based techniques
    Barsa Baisalini Panda, Nitika Pradhan, Rupenangshu K. Hazra
    Molecular Biology Reports.2019; 46(1): 151.     CrossRef
  • Comparison between different methods of DNA isolation from dried blood spots for determination of malaria to determine specificity and cost effectiveness
    Barsa Baisalini Panda, Arup Shankar Meher, Rupenangshu Kumar Hazra
    Journal of Parasitic Diseases.2019; 43(3): 337.     CrossRef
  • All-in-one paper-based sampling chip for targeted protein analysis
    Øystein Skjærvø, Trine Grønhaug Halvorsen, Léon Reubsaet
    Analytica Chimica Acta.2019; 1089: 56.     CrossRef
  • Rapid electrophoretic recovery of DNA from dried blood spots
    Mary C. Machado, Gina V. Vimbela, Michael Nilsson, Stephanie Dallaire, Rongcong Wu, Anubhav Tripathi
    ELECTROPHORESIS.2019; 40(14): 1812.     CrossRef
  • Association of adiponectin gene ( ADIPOQ ) polymorphisms with measures of obesity in Nigerian young adults
    Olusegun E. Ogundele, Khalid O. Adekoya, Abraham A.A. Osinubi, Awoyemi A. Awofala, Bola O. Oboh
    Egyptian Journal of Medical Human Genetics.2018; 19(2): 123.     CrossRef
  • Biobanking in Precision Medicine
    Shanavaz Nasarabadi, Michael Hogan, James Nelson
    Current Pharmacology Reports.2018; 4(1): 91.     CrossRef
  • Can malaria rapid diagnostic tests by drug sellers under field conditions classify children 5 years old or less with or without Plasmodium falciparum malaria? Comparison with nested PCR analysis
    Freddy Eric Kitutu, Henry Wamani, Katarina Ekholm Selling, Fred Ashaba Katabazi, Ronald Bisaso Kuteesa, Stefan Peterson, Joan Nakayaga Kalyango, Andreas Mårtensson
    Malaria Journal.2018;[Epub]     CrossRef
  • Current Nucleic Acid Extraction Methods and Their Implications to Point-of-Care Diagnostics
    Nasir Ali, Rita de Cássia Pontello Rampazzo, Alexandre Dias Tavares Costa, Marco Aurelio Krieger
    BioMed Research International.2017; 2017: 1.     CrossRef
  • Evaluating genomic DNA extraction methods from human whole blood using endpoint and real-time PCR assays
    Linda Koshy, A. L. Anju, S. Harikrishnan, V. R. Kutty, V. T. Jissa, Irin Kurikesu, Parvathy Jayachandran, A. Jayakumaran Nair, A. Gangaprasad, G. M. Nair, P. R. Sudhakaran
    Molecular Biology Reports.2017; 44(1): 97.     CrossRef
  • Detection of <i>β</i>-Hemoglobin Gene and Sickle Cell Disorder from Umbilical Cord Blood
    Sheikh Anika Rahman, Md. Shad Ebna Rahaman, Shahena Aktar Shipa, Md. Mohosin Rana, Rukshana Khan, Mohammad Golam Rob Mahmud, Jilwatun Noor, Firuza Sultana, Md. Faruque Miah
    Journal of Biosciences and Medicines.2017; 05(10): 51.     CrossRef
  • Dosage of Sex Chromosomal Genes in Blood Deposited on Filter Paper for Neonatal Screening of Sex Chromosome Aneuploidy
    Luis Daniel Campos-Acevedo, Marisol Ibarra-Ramirez, José de Jesús Lugo-Trampe, Michelle de Jesús Zamudio-Osuna, Iris Torres-Muñoz, Ma del Roble Velasco-Campos, Luz Rojas-Patlan, Irám Pablo Rodríguez-Sánchez, Laura Elia Martínez-de-Villarreal
    Genetic Testing and Molecular Biomarkers.2016; 20(12): 786.     CrossRef
  • Single Lysis‐Salting Out Method of Genomic DNA Extraction From Dried Blood Spots
    Muntaj Shaik, Devaraju Kuramkote Shivanna, Mahesh Kamate, Vedamurthy AB, Kruthika‐Vinod TP
    Journal of Clinical Laboratory Analysis.2016; 30(6): 1009.     CrossRef
  • Evaluation of artemether-lumefantrine efficacy in the treatment of uncomplicated malaria and its association with pfmdr1, pfatpase6 and K13-propeller polymorphisms in Luanda, Angola
    Kinanga Kiaco, Joana Teixeira, Marta Machado, Virgílio do Rosário, Dinora Lopes
    Malaria Journal.2015;[Epub]     CrossRef

PHRP : Osong Public Health and Research Perspectives