- Comparison of Three Different Methods for Detection of IL28 rs12979860 Polymorphisms as a Predictor of Treatment Outcome in Patients with Hepatitis C Virus
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Abolfazl Fateh, Mohammadreza Aghasadeghi, Seyed D. Siadat, Farzam Vaziri, Farzin Sadeghi, Roohollah Fateh, Hossein Keyvani, Alireza H. Tasbiti, Shamsi Yari, Angila Ataei-Pirkooh, Seyed H. Monavari
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Osong Public Health Res Perspect. 2016;7(2):83-89. Published online April 30, 2016
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DOI: https://doi.org/10.1016/j.phrp.2015.11.004
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3,934
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Abstract
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- Objectives
This study aimed to evaluate the specificity, sensitivity, cost, and turn-around time of three methods of gene polymorphism analysis and to study the relationship between IL28B rs12979860 and SVR rate to pegIFN-α/RVB therapy among patients with chronic hepatitis C. Methods
A total of 100 samples from chronic hepatitis C patients were analyzed in parallel using the three methods: direct sequencing, real-time polymerase chain reaction (PCR), amplification refractory mutation system (ARMS)-PCR. Results
The different profiles for IL28B rs12979860 alleles (CC, CT, and TT) obtained with PCR-RFLP, ARMS-PCR, and direct sequencing were consistent among the three methods. Prevalence of rs12979860 genotypes CC, CT and TT in HCV genotype 1a was 10(19.6%), 35(68.6%), and six (11.8%), respectively, and in HCV genotype 31, it was 13(26.5%), 31(63.3%), and five (10.2%), respectively. No significant difference was seen between rs12979860 genotype and HCV genotype (p = 0.710). Conclusion
Screening by ARMS – PCR SNOP detection represents the most efficient and reliable method to determine HCV polymorphisms in routine clinical practice.
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Citations
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Pegah Namdar Aligoodarzi, Golale Rostami, Seyed Reza Kazemi Nezhad, Mohammad Hamid Iranian Biomedical Journal.2023; 27(1): 46. CrossRef -
Expression of
TRIM56
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Rezvan Tavakoli, Pooneh Rahimi, Mojtaba Hamidi-Fard, Sana Eybpoosh, Delaram Doroud, Iraj Ahmadi, Enayat Anvari, Mohammadreza Aghasadeghi, Abolfazl Fateh Future Virology.2023; 18(9): 563. CrossRef - Performance of the tetra-primer PCR technique compared to PCR-RFLP in the search for rs12979860 (C/T) and rs8099917 (T/G) single nucleotide polymorphisms (SNPs) in the IFNL4 gene
Ellen Hochleitner Souza Kindermann, Karoline Rodrigues Campos, Adele Caterino-de-Araujo Revista do Instituto Adolfo Lutz.2023; 82: 1. CrossRef - Glioblastoma as a Novel Drug Repositioning Target: Updated State
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Ellen Hochleitner Souza Kindermann, Karoline Rodrigues Campos, Adele Caterino-de-Araujo Revista do Instituto Adolfo Lutz.2023; 82: 1. CrossRef - MicroRNAs Profiling in HIV, HCV, and HIV/HCV Co-Infected Patients
Mohsen Moghoofei, Sohrab Najafipour, Shayan Mostafaei , Ahmad Tavakoli , Farah Bokharaei-Salim , Saied Ghorbani, Davod Javanmard, Hadi Ghaffari , Seyed Hamidreza Monavari Current HIV Research.2021; 19(1): 27. CrossRef - Occult hepatitis C virus infection in hemophilia patients and its correlation with interferon lambda 3 and 4 polymorphisms
Amir Hossein Nafari, Ahmad Ayadi, Zahra Noormohamadi, Fatemeh Sakhaee, Farzam Vaziri, Seyed Davar Siadat, Abolfazl Fateh Infection, Genetics and Evolution.2020; 79: 104144. CrossRef - Polymerase Chain Reaction Assay Using the Restriction Fragment Length Polymorphism Technique in the Detection of Prosthetic Joint Infections: A Multi-Centered Study
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Milad Nafari, Shiva Irani, Farzam Vaziri, Safoora Gharibzadeh, Fatemeh Sakhaee, Mohammad Khazeni, Naser Kalhor, Fatemeh Rahimi Jamnani, Seyed Davar Siadat, Abolfazl Fateh Infection, Genetics and Evolution.2018; 62: 296. CrossRef - First detection of human hepegivirus-1 (HHpgV-1) in Iranian patients with hemophilia
Yazdan Bijvand, Mohammad Reza Aghasadeghi, Fatemeh Sakhaee, Parviz Pakzad, Farzam Vaziri, Alireza Azizi Saraji, Fatemeh Rahimi Jamnani, Seyed Davar Siadat, Abolfazl Fateh Scientific Reports.2018;[Epub] CrossRef - Evaluation of TRIM5 and TRIM22 polymorphisms on treatment responses in Iranian patients with chronic hepatitis C virus infection
Setareh Mobasheri, Nazanin Irani, Abbas Akhavan Sepahi, Fatemeh Sakhaee, Fatemeh Rahimi Jamnani, Farzam Vaziri, Seyed Davar Siadat, Abolfazl Fateh Gene.2018; 676: 95. CrossRef - Data Mining and Machine Learning Algorithms Using IL28B Genotype and Biochemical Markers Best Predicted Advanced Liver Fibrosis in Chronic Hepatitis C
Hend Ibrahim Shousha, Abubakr Hussein Awad, Dalia Abdelhamid Omran, Mayada Mohamed Elnegouly, Mahasen Mabrouk Japanese Journal of Infectious Diseases.2018; 71(1): 51. CrossRef - IL28B rs12980275 and HLA rs4273729 genotypes as a powerful predictor factor for rapid, early, and sustained virologic response in patients with chronic hepatitis C
Parvaneh Sedighimehr, Shiva Irani, Fatemeh Sakhaee, Farzam Vaziri, Mohammadreza Aghasadeghi, Seyed Mehdi Sadat, Fatemeh Rahimi Jamnani, Abolfazl Fateh, Seyed Davar Siadat Archives of Virology.2017; 162(1): 181. CrossRef - Comparison of Direct Sequencing, Real-Time PCR-High Resolution Melt (PCR-HRM) and PCR-Restriction Fragment Length Polymorphism (PCR-RFLP) Analysis for Genotyping of Common Thiopurine Intolerant Variant Alleles NUDT15 c.415C>T and TPMT c.719A>G (TPMT*3C)
Wai-Ying Fong, Chi-Chun Ho, Wing-Tat Poon Diagnostics.2017; 7(2): 27. CrossRef - Effect of IL15 rs10833 and SCARB1 rs10846744 on virologic responses in chronic hepatitis C patients treated with pegylated interferon-α and ribavirin
Sahar Sadeghi, Mehdi Davari, Esmaeil Asli, Safoora Gharibzadeh, Farzam Vaziri, Fatemeh Rahimi Jamnani, Abolfazl Fateh, Seyed Davar Siadat Gene.2017; 630: 28. CrossRef - High platelet count and high probability of CALR detection in myeloproliferative neoplasms
Reza Shirzad, Zari Tahan-nejad, Javad Mohamadi-asl, Mohammad Seghatoleslami, Ahmad Ahmadzadeh, Amal Saki Malehi, Najmaldin Saki Comparative Clinical Pathology.2017; 26(1): 25. CrossRef - A comparative study of various methods for detection ofIL28Brs12979860 in chronic hepatitis C
Seyed Hamidreza Monavari, Roohollah Fateh, Farzam Vaziri, Fatemeh Rahimi Jamnani, Enayat Anvari, Farzin Sadeghi, Parviz Afrough, Ava Behrouzi, Fatemeh Sakhaee, Sepideh Meidaninikjeh, Hamidreza Mollaie, Alireza Hadizadeh Tasbiti, Shamsi Yari, Maryam Sadegh Scandinavian Journal of Clinical and Laboratory In.2017; 77(4): 247. CrossRef - EGFR rs11506105 and IFNL3 SNPs but not rs8099917 are strongly associated with treatment responses in Iranian patients with chronic hepatitis C
M Asnavandi, M Zargar, F Vaziri, F R Jamnani, S Gharibzadeh, A Fateh, S D Siadat Genes & Immunity.2017; 18(3): 144. CrossRef - Genetic Variation in Interleukin-28B and Response to Peg-IFNα-2a/RBV Combination Therapy in Patients with Hepatitis C Virus Infection
Farah Bokharaei-Salim, Mostafa Salehi-Vaziri, Farzin Sadeghi, Khadijeh Khanaliha, Maryam Esghaei, Seyed Hamidreza Monavari, Seyed Moayed Alavian, Shahin Fakhim, Hossein Keyvani Jundishapur Journal of Microbiology.2016;[Epub] CrossRef
- Preparation and Evaluation of a New Lipopolysaccharide-based Conjugate as a Vaccine Candidate for Brucellosis
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Seyed Davar Siadat, Farzam Vaziri, Mamak Eftekhary, Maryam Karbasian, Arfa Moshiri, Mohammad R. Aghasadeghi, Mehdi S. Ardestani, Meghdad Abdollahpour Alitappeh, Amin Arsang, Abolfazl Fateh, Shahin Najar Peerayeh, Ahmad R. Bahrmand
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Osong Public Health Res Perspect. 2015;6(1):9-13. Published online February 28, 2015
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DOI: https://doi.org/10.1016/j.phrp.2014.10.012
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4,679
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7
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Abstract
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- Objectives
Development of an efficacious vaccine against brucellosis has been a challenge for scientists for many years. At present, there is no licensed vaccine against human brucellosis. To overcome this problem, currently, antigenic determinants of Brucella cell wall such as Lipopolysaccharide (LPS) are considered as potential candidates to develop subunit vaccines. Methods
In this study, Brucella abortus LPS was used for conjugation to Neisseria meningitidis serogroup B outer membrane vesicle (OMV) as carrier protein using carbodiimide and adipic acid–mediated coupling and linking, respectively. Groups of eight BALB/c mice were injected subcutaneously with 10 μg LPS alone, combined LPS + OMV and conjugated LPS–OMV on 0 days, 14 days, 28 days and 42 days. Anti-LPS IgG was measured in serum. Results
The yield of LPS to OMV in LPS–OMV conjugate was 46.55%, on the basis of carbohydrate content. The ratio for LPS to OMV was 4.07. The LPS–OMV conjugate was the most immunogenic compound that stimulated following the first injection with increased IgG titer of ∼5-fold and ∼1.3-fold higher than that produced against LPS and LPS in noncovalent complex to OMV (LPS + OMV), respectively. The highest anti-LPS IgG titer was detected 2 weeks after the third injection (Day 42) of LPS–OMV conjugate. The conjugated compound elicited higher titers of IgG than LPS + OMV, that showed a 100–120-fold rise of anti-LPS IgG in mice. Conclusion
These results indicate that our conjugated LPS–OMV can be used as a brucellosis vaccine, but further investigation is required.
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Citations
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- Development of a colloidal gold immunochromatographic test strip for detecting the smooth Brucella
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Qiong Long, Peng Zheng, Xiao Zheng, Weiran Li, Liangqun Hua, Zhongqian Yang, Weiwei Huang, Yanbing Ma Advanced Drug Delivery Reviews.2022; 186: 114321. CrossRef - Isolation of Extracellular Vesicles of Akkermansia muciniphila as a Potential Therapeutic Platform
Pegah Noori, Fattah Sotoodehnejadnematalali, Pooneh Rahimi, Seyed davar Siadat Vaccine Research.2022; 9(2): 27. CrossRef - Novel Simple Conjugation Chemistries for Decoration of GMMA with Heterologous Antigens
Roberta Di Benedetto, Renzo Alfini, Martina Carducci, Maria Aruta, Luisa Lanzilao, Alessandra Acquaviva, Elena Palmieri, Carlo Giannelli, Francesca Necchi, Allan Saul, Francesca Micoli International Journal of Molecular Sciences.2021; 22(19): 10180. CrossRef - Outer membrane vesicle vaccines
Francesca Micoli, Calman A. MacLennan Seminars in Immunology.2020; 50: 101433. CrossRef -
Designing an immunosensor for detection of
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Matthias J.H. Gerritzen, Dirk E. Martens, René H. Wijffels, Leo van der Pol, Michiel Stork Biotechnology Advances.2017; 35(5): 565. CrossRef
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