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Hamed Tahmasebi 1 Article
Association between Beta-lactam Antibiotic Resistance and Virulence Factors in AmpC Producing Clinical Strains of P. aeruginosa
Sanaz Dehbashi, Hamed Tahmasebi, Mohammad Reza Arabestani
Osong Public Health Res Perspect. 2018;9(6):325-333.   Published online December 31, 2018
DOI: https://doi.org/10.24171/j.phrp.2018.9.6.06
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  • 12 Citations
AbstractAbstract PDF
Objectives

The purpose of this study was to determine the presence of IMP and OXA genes in clinical strains of Pseudomonas aeruginosa (P. aeruginosa) that are carriers of the ampC gene.

Methods

In this study, 105 clinical isolates of P. aeruginosa were collected. Antibiotic resistance patterns were determined using the disk diffusion method. The strains carrying AmpC enzymes were characterized by a combination disk method. Multiplex-PCR was used to identify resistance and virulence genes, chi-square test was used to determine the relationship between variables.

Results

Among 105 isolates of P. aeruginosa, the highest antibiotic resistance was to cefotaxime and aztreonam, and the least resistance was to colictin and ceftazidime. There were 49 isolates (46.66%) that showed an AmpC phenotype. In addition, the frequencies of the resistance genes were; OXA48 gene 85.2%, OXA199, 139 3.8%, OXA23 3.8%, OXA2 66.6%, OXA10 3.8%, OXA51 85.2% and OXA58 3.8%. The IMP27 gene was detected in 9 isolates (8.57%) and the IMP3.34 was detected in 11 isolates (10.47%). Other genes detected included; lasR (17.1%), lasB (18%) and lasA (26.6%). There was a significant relationship between virulence factors and the OX and IMP genes (p ≤ 0.05).

Conclusion

The relationship between antibiotic resistance and virulence factors observed in this study could play an important role in outbreaks associated with P. aeruginosa infections.

Citations

Citations to this article as recorded by  
  • Molecular epidemiology and collaboration of siderophore-based iron acquisition with surface adhesion in hypervirulent Pseudomonas aeruginosa isolates from wound infections
    Hamed Tahmasebi, Sanaz Dehbashi, Mona Nasaj, Mohammad Reza Arabestani
    Scientific Reports.2022;[Epub]     CrossRef
  • Decoding Genetic Features and Antimicrobial Susceptibility of Pseudomonas aeruginosa Strains Isolated from Bloodstream Infections
    Tomasz Bogiel, Dagmara Depka, Mateusz Rzepka, Agnieszka Mikucka
    International Journal of Molecular Sciences.2022; 23(16): 9208.     CrossRef
  • Prevalence of the Genes Associated with Biofilm and Toxins Synthesis amongst the Pseudomonas aeruginosa Clinical Strains
    Tomasz Bogiel, Dagmara Depka, Mateusz Rzepka, Joanna Kwiecińska-Piróg, Eugenia Gospodarek-Komkowska
    Antibiotics.2021; 10(3): 241.     CrossRef
  • A Comprehensive Study of the Relationship between the Production of β-Lactamase Enzymes and Iron/Siderophore Uptake Regulatory Genes in Clinical Isolates of Acinetobacter baumannii
    Mahyar Porbaran, Hamed Tahmasebi, MohammadReza Arabestani, Joseph Falkinham
    International Journal of Microbiology.2021; 2021: 1.     CrossRef
  • Regulation of virulence and β-lactamase gene expression in Staphylococcus aureus isolates: cooperation of two-component systems in bloodstream superbugs
    Sanaz Dehbashi, Hamed Tahmasebi, Behrouz Zeyni, Mohammad Reza Arabestani
    BMC Microbiology.2021;[Epub]     CrossRef
  • New approach to identify colistin‐resistant Pseudomonas aeruginosa by high‐resolution melting curve analysis assay
    H. Tahmasebi, S. Dehbashi, M.R. Arabestani
    Letters in Applied Microbiology.2020; 70(4): 290.     CrossRef
  • Resistant Pseudomonas aeruginosa carrying virulence genes in hospitalized patients with urinary tract infection from Sanandaj, west of Iran
    Safoura Derakhshan, Aslan Hosseinzadeh
    Gene Reports.2020; 20: 100675.     CrossRef
  • Prevalence and molecular typing of Metallo-β-lactamase-producing Pseudomonas aeruginosa with adhesion factors: A descriptive analysis of burn wounds isolates from Iran
    Hamed Tahmasebi, Sanaz Dehbashi, Mohammad Yousef Alikhani, Mahyar Porbaran, Mohammad Reza Arabestani
    Gene Reports.2020; 21: 100853.     CrossRef
  • Co-harboring of mcr-1 and β-lactamase genes in Pseudomonas aeruginosa by high-resolution melting curve analysis (HRMA): Molecular typing of superbug strains in bloodstream infections (BSI)
    Hamed Tahmasebi, Sanaz Dehbashi, Mohammad Reza Arabestani
    Infection, Genetics and Evolution.2020; 85: 104518.     CrossRef
  • Relationship between Biofilm Regulating Operons and Various Β-Lactamase Enzymes: Analysis of the Clinical Features of Infections caused by Non-Fermentative Gram-Negative Bacilli (Nfgnb) from Iran
    Mahyar Porbaran, Reza Habibipour
    Journal of Pure and Applied Microbiology.2020; 14(3): 1723.     CrossRef
  • Carbapenem-Resistant Pseudomonas aeruginosa Strains-Distribution of the Essential Enzymatic Virulence Factors Genes
    Tomasz Bogiel, Małgorzata Prażyńska, Joanna Kwiecińska-Piróg, Agnieszka Mikucka, Eugenia Gospodarek-Komkowska
    Antibiotics.2020; 10(1): 8.     CrossRef
  • Biofilm Formation and β-lactamase Enzymes: A Synergism Activity in Acinetobacter baumannii Isolated from Wound Infection
    Mahyar Porbaran, Reza Habibipour
    Journal of Advances in Medical and Biomedical Rese.2019; 27(125): 34.     CrossRef

PHRP : Osong Public Health and Research Perspectives