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Hee-Bok Oh 3 Articles
Serological Correlate of Protection in Guinea Pigs for a Recombinant Protective Antigen Anthrax Vaccine Produced from Bacillus brevis
Jeong-Hoon Chun, On-Jee Choi, Min-Hee Cho, Kee-Jong Hong, Won Keun Seong, Hee-Bok Oh, Gi-Eun Rhie
Osong Public Health Res Perspect. 2012;3(3):170-176.   Published online June 30, 2012
DOI: https://doi.org/10.1016/j.phrp.2012.07.006
  • 2,187 View
  • 22 Download
  • 10 Citations
AbstractAbstract PDF
Objective Recombinant protective antigen (rPA) is the active pharmaceutical ingredient of a second generation anthrax vaccine undergoing clinical trials both in Korea and the USA. By using the rPA produced from Bacillus brevis pNU212 expression system, correlations of serological immune response to anthrax protection efficacy were analyzed in a guinea pig model.
Methods
Serological responses of rPA anthrax vaccine were investigated in guinea pigs that were given single or two injections (interval of 4 weeks) of various amounts of rPA combined with aluminumhydroxide adjuvant. Guinea pigs were subsequently challenged by the intramuscular injection with 30 half-lethal doses (30LD50) of virulent Bacillus anthracis spores. Serumantibody titerswere determined by anti-PA IgGELISA and the ability of antibodies to neutralize the cytotoxicity of lethal toxin on J774A.1 cell was measured through the toxin neutralizing antibody (TNA) assay.
Results
To examine correlations between survival rate and antibody titers, correlation between neutralizing antibody titers and the extent of protection was determined. Toxin neutralization titers of at least 1176 were sufficient to confer protection against a dose of 30LD50 of virulent anthrax spores of the H9401 strain. Such consistency in the correlation was not observed from those antibody titers determined by ELISA.
Conclusion
Neutralizing-antibody titers can be used as a surrogate marker.

Citations

Citations to this article as recorded by  
  • A putative exosporium lipoprotein GBAA0190 of Bacillus anthracis as a potential anthrax vaccine candidate
    Jun Ho Jeon, Yeon Hee Kim, Kyung Ae Kim, Yu-Ri Kim, Sun-Je Woo, Ye Jin Choi, Gi-eun Rhie
    BMC Immunology.2021;[Epub]     CrossRef
  • Construction of a bivalent vaccine against anthrax and smallpox using the attenuated vaccinia virus KVAC103
    Deok Bum Park, Bo-Eun Ahn, Hosun Son, Young-Ran Lee, Yu-Ri Kim, Su Kyoung Jo, Jeong-Hoon Chun, Jae-Yon Yu, Myung-Min Choi, Gi-eun Rhie
    BMC Microbiology.2021;[Epub]     CrossRef
  • Single-dose combination nanovaccine induces both rapid and durable humoral immunity and toxin neutralizing antibody responses against Bacillus anthracis
    Sean M. Kelly, Kristina R. Larsen, Ross Darling, Andrew C. Petersen, Bryan H. Bellaire, Michael J. Wannemuehler, Balaji Narasimhan
    Vaccine.2021; 39(29): 3862.     CrossRef
  • Current Status and Trends in Prophylaxis and Management of Anthrax Disease
    Vladimir Savransky, Boris Ionin, Joshua Reece
    Pathogens.2020; 9(5): 370.     CrossRef
  • Anthrax prevention through vaccine and post-exposure therapy
    Manish Manish, Shashikala Verma, Divya Kandari, Parul Kulshreshtha, Samer Singh, Rakesh Bhatnagar
    Expert Opinion on Biological Therapy.2020; 20(12): 1405.     CrossRef
  • A therapeutic human antibody against the domain 4 of the Bacillus anthracis protective antigen shows protective efficacy in a mouse model
    Bo-Eun Ahn, Hee-Won Bae, Hae-Ri Lee, Sun-Je Woo, Ok-Kyu Park, Jun Ho Jeon, Jungchan Park, Gi-eun Rhie
    Biochemical and Biophysical Research Communication.2019; 509(2): 611.     CrossRef
  • Vaccines against anthrax based on recombinant protective antigen: problems and solutions
    Olga A. Kondakova, Nikolai A. Nikitin, Ekaterina A. Evtushenko, Ekaterina M. Ryabchevskaya, Joseph G. Atabekov, Olga V. Karpova
    Expert Review of Vaccines.2019; 18(8): 813.     CrossRef
  • A novel live attenuated anthrax spore vaccine based on an acapsular Bacillus anthracis Sterne strain with mutations in the htrA, lef and cya genes
    Theodor Chitlaru, Ma'ayan Israeli, Shahar Rotem, Uri Elia, Erez Bar-Haim, Sharon Ehrlich, Ofer Cohen, Avigdor Shafferman
    Vaccine.2017; 35(44): 6030.     CrossRef
  • Expression and refolding of the protective antigen of Bacillus anthracis: A model for high-throughput screening of antigenic recombinant protein refolding
    María Elisa Pavan, Esteban Enrique Pavan, Fabián Martín Cairó, María Julia Pettinari
    Revista Argentina de Microbiología.2016; 48(1): 5.     CrossRef
  • Protein- and DNA-based anthrax toxin vaccines confer protection in guinea pigs against inhalational challenge withBacillus cereusG9241
    John Palmer, Matt Bell, Christian Darko, Roy Barnewall, Andrea Keane-Myers
    Pathogens and Disease.2014; : n/a.     CrossRef
Pathogenesis and Chronologic Localization of the Human Influenza A (H1N1) Virus in Cotton Rats
Donghyok Kwon, Kyeongcheol Shin, Jin-Young Shin, Joo-Yeon Lee, Yooncheol Ha, Nam-Joo Lee, Hee-Bok Oh, Chanhee Chae, Chun Kang
Osong Public Health Res Perspect. 2011;2(1):15-22.   Published online June 30, 2011
DOI: https://doi.org/10.1016/j.phrp.2011.04.005
  • 1,946 View
  • 22 Download
  • 7 Citations
AbstractAbstract PDF
Objectives
We aimed to evaluate the pathogenesis and chronologic localization of human influenza A (H1N1) virus in experimentally infected cotton rats.
Methods
The animals were intranasally inoculated with 107 plaque-forming units of A/Solomon Islands/3/2006 (H1N1) influenza virus and evaluated for pathogenicity for a period of 28 days. Virus replication kinetics and pathological properties were assessed chronologically. Acute antiviral responses were evaluated by mean of real-time polymerase chain reaction.
Results
Cotton rats infected with A/Solomon Islands/3/2006 virus lost weight until 6 days post-inoculation (DPI) and showed decreased activity until 3 DPI. At necropsy, focal areas of redness and consolidation of lungs were evident at 1, 2, and 3 DPI. Lung histopathology showed moderate to severe interstitial pneumonia, alveolitis and bronchiolitis. Influenza A specific viral protein was detected in bronchiolar epithelial cells, alveolar septa and pneumocytes. Influenza viruses were recovered from the lungs during the early period of infection and the titer peaked at 1 DPI. Viral proteins were detected from 4 hours to 6 hours DPI. These trends correlate with the up-regulation of mRNA expression of the IFN-α, Mx1, and Mx2 genes that play critical roles in the anti-influenza response at the early stage of infection.
Conclusion
Our results provide evidence that supports the use of cotton rats for the study of influenza virus pathogenesis and the immune response.

Citations

Citations to this article as recorded by  
  • Characterization of humoral immune responses and degree of protection induced by influenza vaccine in cotton rats: Effects of low vaccine dose and single vs booster vaccination
    Yoshita Bhide, Wei Dong, Tjarko Meijerhof, Jacqueline Vries‐Idema, Hubert G. Niesters, Anke Huckriede
    Immunity, Inflammation and Disease.2020; 8(3): 279.     CrossRef
  • Doing Mathematics with Aftermath of Pandemic Influenza 2009
    Hae-Wol Cho, Chaeshin Chu
    Osong Public Health and Research Perspectives.2015; 6(1): 1.     CrossRef
  • Assessment of Intensive Vaccination and Antiviral Treatment in 2009 Influenza Pandemic in Korea
    Chaeshin Chu, Sunmi Lee
    Osong Public Health and Research Perspectives.2015; 6(1): 47.     CrossRef
  • Mammalian pathogenesis of oseltamivir-resistant pandemic (H1N1) 2009 influenza virus isolated in South Korea
    Donghyok Kwon, Kyeongcheol Shin, Su-Jin Kim, Joo-Yeon Lee, Chun Kang
    Virus Research.2014; 185: 41.     CrossRef
  • Was the Mass Vaccination Effective During the Influenza Pandemic 2009–2010 in Korea?
    Hae-Wol Cho, Chaeshin Chu
    Osong Public Health and Research Perspectives.2013; 4(4): 177.     CrossRef
  • How to Manage a Public Health Crisis and Bioterrorism in Korea
    Hae-Wol Cho, Chaeshin Chu
    Osong Public Health and Research Perspectives.2013; 4(5): 223.     CrossRef
  • The Road Less Traveled
    Chaeshin Chu
    Osong Public Health and Research Perspectives.2011; 2(1): 1.     CrossRef
Distribution of Virulence Genes and Their Association of Serotypes in Pathogenic Escherichia coli Isolates From Diarrheal Patients in Korea
Seung-Hak Cho, Kyung-Hwan Oh, Seong-Han Kim, Hee-Bok Oh, Mi-Sun Park
Osong Public Health Res Perspect. 2010;1(1):29-35.   Published online December 31, 2010
DOI: https://doi.org/10.1016/j.phrp.2010.12.008
  • 2,119 View
  • 15 Download
  • 6 Citations
AbstractAbstract PDF
Objectives
To characterise the genetic and serological diversity of pathogenic Escherichia coli, we tested 111 E coli strains isolated from diarrhoeal patients in Korea between 2003 and 2006.
Methods
The isolates were tested through polymerase chain reaction (PCR) and slide agglutination method for the detection of virulence genes and serotypes, respectively. To compare the expression of Shiga toxin (stx)-1 and stx2 genes, real-time quantitative reverse-transcriptase PCR and rapid exprssion assay, reversed-passive latex agglutination, were performed.
Results
Forty-nine Shiga toxin-producing E coli (STEC) strains and 62 non-STEC strains, including 20 enteropathogenic E coli, 20 enterotoxigenic E coli, 20 enteroaggregative E coli, and 2 enteroinvasive E coli were randomly chosen from the strains isolated from diarrhoeal patients in Korea between 2003 and 2006. PCR analysis indicated that locus of enterocyte effacement pathogenicity island, that is, eaeA, espADB, and tir genes were present in STEC, enteropathogenic E coli, and enteroinvasive E coli. Quorum sensing-related gene luxS was detected in most of pathogenic E coli strains. Major serotypes of the STEC strains were O157 (26%) and O26 (20%), whereas the non-STEC strains possessed various serotypes. Especially, all the strains with serotype O157 carried stx2 and the tested virulence factors. Of the STEC strains, the data of real-time quantitative reverse-transcriptase PCR and reversed-passive latex agglutination tests showed that messenger RNA- and protein expression of stx2 gene were higher than those of stx1 gene.
Conclusion
Our results provide the epidemiological information regarding the trend of STEC and non-STEC infections in the general population and show the fundamental data in association of serotypes with virulence genes in diarrhoeagenic E coli strains from Korea.

Citations

Citations to this article as recorded by  
  • Comparative Analysis of Human and Animal E. coli: Serotyping, Antimicrobial Resistance, and Virulence Gene Profiling
    Mahmoud M. Bendary, Marwa I. Abdel-Hamid, Walaa A. Alshareef, Hanan M. Alshareef, Rasha A. Mosbah, Nasreen N. Omar, Mohammad M. Al-Sanea, Majid Alhomrani, Abdulhakeem S. Alamri, Walaa H. Moustafa
    Antibiotics.2022; 11(5): 552.     CrossRef
  • Antimicrobial peptide human β-defensin-2 improves in vitro cellular viability and reduces pro-inflammatory effects induced by enteroinvasive Escherichia coli in Caco-2 cells by inhibiting invasion and virulence factors’ expression
    Alessandra Fusco, Vittoria Savio, Brunella Perfetto, Roberto Mattina, Giovanna Donnarumma
    Frontiers in Cellular and Infection Microbiology.2022;[Epub]     CrossRef
  • Distribution of Pathogenicity Island Markers and H-Antigen Types of Escherichia coli O25b/ST131 Isolates from Patients with Urinary Tract Infection in Iran
    Masoumeh Rasoulinasab, Fereshteh Shahcheraghi, Mohammad Mehdi Feizabadi, Bahram Nikmanesh, Azade Hajihasani, Shahram Sabeti, Mohammad Mehdi Aslani
    Microbial Drug Resistance.2021; 27(3): 369.     CrossRef
  • Development and validation of a predictive model for pathogenic Escherichia coli in fresh‐cut produce
    You Jin Kim, Ju Yeon Park, Soo Hwan Suh, Mi‐Gyeong Kim, Hyo‐Sun Kwak, Soon Han Kim, Eun Jeong Heo
    Food Science & Nutrition.2021; 9(12): 6866.     CrossRef
  • In vitro antibacterial activity of poly (amidoamine)-G7 dendrimer
    Mitra Gholami, Rashin Mohammadi, Mohsen Arzanlou, Fakhraddin Akbari Dourbash, Ebrahim Kouhsari, Gharib Majidi, Seyed Mohsen Mohseni, Shahram Nazari
    BMC Infectious Diseases.2017;[Epub]     CrossRef
  • Occurrence of pathogenic Escherichia coli in commercially available fresh vegetable products in Korea
    Hyun Jung Kim, Minseon Koo, A-Ram Jeong, Seung-Youb Baek, Joon-Il Cho, Soon-Ho Lee, In-Gyun Hwang
    Journal of the Korean Society for Applied Biologic.2014; 57(3): 367.     CrossRef

PHRP : Osong Public Health and Research Perspectives