- Identification of Klebsiella pneumoniae Carbapenemase-producing Klebsiella oxytoca in Clinical Isolates in Tehran Hospitals, Iran by Chromogenic Medium and Molecular Methods
-
Majid Validi, Mohammad Mehdi Soltan Dallal, Masoumeh Douraghi, Jalil Fallah Mehrabadi, Abbas Rahimi Foroushani
-
Osong Public Health Res Perspect. 2016;7(5):301-306. Published online October 31, 2016
-
DOI: https://doi.org/10.1016/j.phrp.2016.08.006
-
-
1,789
View
-
25
Download
-
8
Citations
-
 Abstract
 PDF
- Objectives
Production of carbapenemase, especially Klebsiella pneumoniae carbapenemases (KPC), is one of the antibiotic resistance mechanisms of Enterobacteriaceae such as Klebsiella oxytoca. This study aimed to investigate and identify KPC-producing K. oxytoca isolates using molecular and phenotypic methods.
Methods
A total of 75 isolates of K. oxytoca were isolated from various clinical samples, and were verified as K. oxytoca after performing standard microbiological tests and using a polymerase chain reaction (PCR) method. An antibiotic susceptibility test was performed using a disc diffusion method according to the Clinical and Laboratory Standards Institute guidelines. CHROMagar KPC chromogenic culture media was used to examine and confirm the production of the carbapenemase enzyme in K. oxytoca isolates; in addition, PCR was used to evaluate the presence of blaKPC gene in K. oxytoca strains.
Results
Of a total of 75 K. oxytoca isolates, one multidrug resistant strain was isolated from the urine of a hospitalized woman. This strain was examined to assess its ability to produce carbapenemase enzyme; it produced a colony with a blue metallic color on the CHROMagar KPC chromogenic culture media. In addition, the blaKPC gene was confirmed by PCR. After sequencing, it was confirmed and deposited in GenBank.
Conclusion
To date, many cases of KPC-producing Enterobacteriaceae, in particular K. pneumoniae, have been reported in different countries; there are also some reports on the identification of KPC-producing K. oxytoca. Therefore, to prevent the outbreak of nosocomial infections, the early detection, control, and prevention of the spread of these strains are of great importance.
-
Citations
Citations to this article as recorded by  - Klebsiella oxytoca Complex: Update on Taxonomy, Antimicrobial Resistance, and Virulence
Jing Yang, Haiyan Long, Ya Hu, Yu Feng, Alan McNally, Zhiyong Zong
Clinical Microbiology Reviews.2022;[Epub] CrossRef - Development and comparison of immunochromatographic strips with four nanomaterial labels: Colloidal gold, new colloidal gold, multi-branched gold nanoflowers and Luminol-reduced Au nanoparticles for visual detection of Vibrio parahaemolyticus in seafood
Meijiao Wu, Youxue Wu, Cheng Liu, Yachen Tian, Shuiqin Fang, Hao Yang, Bin Li, Qing Liu
Aquaculture.2021; 539: 736563. CrossRef - Variation in Accessory Genes Within the Klebsiella oxytoca Species Complex Delineates Monophyletic Members and Simplifies Coherent Genotyping
Amar Cosic, Eva Leitner, Christian Petternel, Herbert Galler, Franz F. Reinthaler, Kathrin A. Herzog-Obereder, Elisabeth Tatscher, Sandra Raffl, Gebhard Feierl, Christoph Högenauer, Ellen L. Zechner, Sabine Kienesberger
Frontiers in Microbiology.2021;[Epub] CrossRef - Multidrug-Resistant Bacteria and Alternative Methods to Control Them: An Overview
Roberto Vivas, Ana Andréa Teixeira Barbosa, Silvio Santana Dolabela, Sona Jain
Microbial Drug Resistance.2019; 25(6): 890. CrossRef - Molecular typing of cytotoxin-producing Klebsiella oxytoca isolates by 16S-23S internal transcribed spacer PCR
M.M. Soltan Dallal, M. Validi, M. Douraghi, B. Bakhshi
New Microbes and New Infections.2019; 30: 100545. CrossRef - Determination of antibiotic resistance and minimum inhibitory concentration of meropenem and imipenem growth in Klebsiella strains isolated from urinary tract infection in Shahrekord educational hospitals
Farshad Kakian, Behnam Zamzad, Abolfazl Gholipour, Kiarash Zamanzad
Journal of Shahrekord University of Medical Scienc.2019; 21(2): 80. CrossRef - Evaluation the cytotoxic effect of cytotoxin-producing Klebsiella oxytoca isolates on the HEp-2 cell line by MTT assay
Mohammad Mehdi Soltan-Dallal, Majid Validi, Masoumeh Douraghi, Jalil Fallah-Mehrabadi, Leila Lormohammadi
Microbial Pathogenesis.2017; 113: 416. CrossRef - Outbreak by Hypermucoviscous Klebsiella pneumoniae ST11 Isolates with Carbapenem Resistance in a Tertiary Hospital in China
Lingling Zhan, Shanshan Wang, Yinjuan Guo, Ye Jin, Jingjing Duan, Zhihao Hao, Jingnan Lv, Xiuqin Qi, Longhua Hu, Liang Chen, Barry N. Kreiswirth, Rong Zhang, Jingye Pan, Liangxing Wang, Fangyou Yu
Frontiers in Cellular and Infection Microbiology.2017;[Epub] CrossRef
|
