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Seok-Min Yun 2 Articles
Cloning and Expression of Recombinant Tick-Borne Encephalitis Virus-like Particles in Pichia pastoris
Seok-Min Yun, Young Eui Jeong, Eunbyeol Wang, Ye-Ji Lee, Myung Guk Han, Chan Park, Won-Ja Lee, WooYoung Choi
Osong Public Health Res Perspect. 2014;5(5):274-278.   Published online October 31, 2014
DOI: https://doi.org/10.1016/j.phrp.2014.08.005
  • 2,099 View
  • 16 Download
  • 7 Citations
AbstractAbstract PDF
Objectives
The purpose of this study was to verify the feasibility of using the glyceraldehyde-3-phosphate dehydrogenase (GAP) promotor based Pichia pastoris expression system to produce tick-borne encephalitis virus (TBEV) virus-like particles (VLPs).
Methods
The complementary DNA encoding the TBEV prM signal peptide, prM, and E proteins of TBEV Korean strain (KrM 93) was cloned into the plasmid vector pGAPZɑA, then integrated into the genome of P. pastoris, under the control of the GAP promoter. Expression of TBEV VLPs was determined by Western blotting using monoclonal antibody against TBEV envelope (E) protein.
Results
Recombinant TBEV VLPs consisting of prM and E protein were successfully expressed using the GAP promoter-based P. pastoris expression system. The results of Western blotting showed that the recombinant proteins were secreted into the culture supernatant from the P. pastoris and glycosylated.
Conclusion
This study suggests that recombinant TBEV VLPs from P. pastoris offer a promising approach to the production of VLPs for use as vaccines and diagnostic antigens.

Citations

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  • De novo transcriptome sequencing and comparative profiling of the ovary in partially engorged and fully engorged Haemaphysalis flava ticks
    Yu Zhao, Zhe-Hui Qu, Feng-Chao Jiao
    Parasitology International.2021; 83: 102344.     CrossRef
  • Flavivirus vaccines: Virus-like particles and single-round infectious particles as promising alternatives
    Esmeralda Cuevas-Juárez, Victoria Pando-Robles, Laura A. Palomares
    Vaccine.2021; 39(48): 6990.     CrossRef
  • NS1 Recombinant Proteins Are Efficiently Produced in Pichia pastoris and Have Great Potential for Use in Diagnostic Kits for Dengue Virus Infections
    Mariana Fonseca Xisto, John Willians Oliveira Prates, Ingrid Marques Dias, Roberto Sousa Dias, Cynthia Canedo da Silva, Sérgio Oliveira de Paula
    Diagnostics.2020; 10(6): 379.     CrossRef
  • Tick-Borne Encephalitis Virus: A Quest for Better Vaccines against a Virus on the Rise
    Mareike Kubinski, Jana Beicht, Thomas Gerlach, Asisa Volz, Gerd Sutter, Guus F. Rimmelzwaan
    Vaccines.2020; 8(3): 451.     CrossRef
  • Virus-Like Particle Systems for Vaccine Development Against Viruses in the Flaviviridae Family
    Wong, Jassey, Wang, Wang, Liu, Lin
    Vaccines.2019; 7(4): 123.     CrossRef
  • ON MODERN APPROACHES TO CREATION OF A SINGLE-CYCLE VACCINE AGAINST TICK-BORNE ENCEPHALITIS
    V. A. Lashkevich, G. G. Karganova
    Problems of Virology.2018; 63(3): 101.     CrossRef
  • Production of an enzymatically active and immunogenic form of ectodomain of Porcine rubulavirus hemagglutinin-neuraminidase in the yeast Pichia pastoris
    José Luis Cerriteño-Sánchez, Gerardo Santos-López, Nora Hilda Rosas-Murrieta, Julio Reyes-Leyva, Sandra Cuevas-Romero, Irma Herrera-Camacho
    Journal of Biotechnology.2016; 223: 52.     CrossRef
Prevalence of Tick-Borne Encephalitis Virus in Ixodid Ticks Collected from the Republic of Korea During 2011–2012
Seok-Min Yun, Bong Gu Song, WooYoung Choi, Won Il Park, Sung Yun Kim, Jong Yul Roh, Jungsang Ryou, Young Ran Ju, Chan Park, E-Hyun Shin
Osong Public Health Res Perspect. 2012;3(4):213-221.   Published online December 31, 2012
DOI: https://doi.org/10.1016/j.phrp.2012.10.004
  • 2,172 View
  • 24 Download
  • 26 Citations
AbstractAbstract PDF
Objectives
In this study, we demonstrated that TBEV-infected ticks have been distributed in the ROK, combined with our previous results. These results suggest that TBEV may exist in the ROK, and H. longicornis, H. flava, and I. nipponensis may be potential vectors of TBEV. In addition, these results emphasize the need for further epidemiological research of TBEV.
Methods
We examined for the presence of RNA of TBEV by reverse transcriptase-nested polymerase chain reaction (RT-nested PCR) using ixodid ticks captured in 25 localities of 10 provinces. Ticks were collected by the flagging and dragging method or using sentinel BG traps at forests, grass thickets, and grassland. A total of 13,053 ticks belonging to two genera and four species were collected and pooled (1292 pools), according to collection site, species of tick, and developmental stage.
Results
Among 1292 pools, the envelope (E) protein gene of TBEV was detected using RT-nested PCR in 10 pools (3 pools of the 1,331 adult ticks and 7 pools of the 11,169 nymph ticks) collected from Gangwon-do province, Jeonrabuk-do province, and Jeju Island. The minimum infection rates for TBEV of Haemaphysalis longicornis, Haemaphysalis flava, and Ixodes nipponensis were 0.06%, 0.17%, and 2.38%, respectively. Phylogenetic analysis based on the partial E protein gene was performed to identify relationships between the TBEV strains. This showed that 10 Korean strains clustered with the Western subtype.
Conclusion
In this study, we investigated the prevalence of tick-borne encephalitis virus (TBEV) in ixodid ticks from various regions of the Republic of Korea (ROK) during 2011–2012 to identify whether TBEV is circulating and to determine the endemic regions of TBEV.

Citations

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    Xiaohui Li, Hongwei Ji, Di Wang, Lihe Che, Li Zhang, Liang Li, Qing Yin, Quan Liu, Feng Wei, Zedong Wang
    Journal of Medical Virology.2022; 94(2): 507.     CrossRef
  • TBE in South Korea
    Song Joon Young
    Tick-borne encephalitis - The Book.2022;[Epub]     CrossRef
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    Artem N. Bondaryuk, Nina V. Kulakova, Ulyana V. Potapova, Olga I. Belykh, Anzhelika V. Yudinceva, Yurij S. Bukin
    International Journal of Molecular Sciences.2022; 23(21): 13404.     CrossRef
  • TBE in South Korea
    Song Joon Young
    Tick-borne encephalitis - The Book.2021;[Epub]     CrossRef
  • Hard Ticks as Vectors Tested Negative for Severe Fever with Thrombocytopenia Syndrome in Ganghwa-do, Korea during 2019-2020
    Kyoung Jin, Yeon-Ja Koh, Seong Kyu Ahn, Joonghee Cho, Junghwan Lim, Jaeyong Song, Jinyoung Lee, Young Woo Gong, Mun Ju Kwon, Hyung Wook Kwon, Young Yil Bahk, Tong-Soo Kim
    The Korean Journal of Parasitology.2021; 59(3): 281.     CrossRef
  • Nationwide Temporal and Geographical Distribution of Tick Populations and Phylogenetic Analysis of Severe Fever with Thrombocytopenia Syndrome Virus in Ticks in Korea, 2020
    Min-Goo Seo, Byung-Eon Noh, Hak Seon Lee, Tae-Kyu Kim, Bong-Goo Song, Hee Il Lee
    Microorganisms.2021; 9(8): 1630.     CrossRef
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    Ji Yun Noh, Joon Young Song, Joon Yong Bae, Man-Seong Park, Jin Gu Yoon, Hee Jin Cheong, Woo Joo Kim, Nam-Hyuk Cho
    PLOS Neglected Tropical Diseases.2021; 15(8): e0009687.     CrossRef
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    Yu. S. Savinova
    Acta Biomedica Scientifica.2021; 6(4): 100.     CrossRef
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    Andrei A. Deviatkin, Ivan S. Kholodilov, Yulia A. Vakulenko, Galina G. Karganova, Alexander N. Lukashev
    Viruses.2020; 12(2): 247.     CrossRef
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    Yuji Takahashi, Shintaro Kobayashi, Mariko Ishizuka, Minato Hirano, Memi Muto, Shoko Nishiyama, Hiroaki Kariwa, Kentaro Yoshii
    Journal of General Virology.2020; 101(5): 497.     CrossRef
  • A history of the introduction, establishment, dispersal and management ofHaemaphysalis longicornisNeumann, 1901 (Ixodida: Ixodidae) in New Zealand
    Allen C. G. Heath
    New Zealand Journal of Zoology.2020; 47(4): 241.     CrossRef
  • Four Year Surveillance of the Vector Hard Ticks for SFTS, Ganghwa-do, Republic of Korea
    Myung-Deok Kim-Jeon, Seung Jegal, Hojong Jun, Haneul Jung, Seo Hye Park, Seong Kyu Ahn, Jinyoung Lee, Young Woo Gong, Kwangsig Joo, Mun Ju Kwon, Jong Yul Roh, Wook-Gyo Lee, Young Yil Bahk, Tong-Soo Kim
    The Korean Journal of Parasitology.2019; 57(6): 691.     CrossRef
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  • Ixodid ticks and tick-borne encephalitis virus prevalence in the South Asian part of Russia (Republic of Tuva)
    Ivan Kholodilov, Oxana Belova, Ludmila Burenkova, Yuri Korotkov, Lidiya Romanova, Lola Morozova, Vitalii Kudriavtsev, Larissa Gmyl, Ilmira Belyaletdinova, Alexander Chumakov, Natalia Chumakova, Oyumaa Dargyn, Nina Galatsevich, Anatoly Gmyl, Mikhail Mikhai
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  • Molecular characterization of Haemaphysalis longicornis-borne rickettsiae, Republic of Korea and China
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  • Molecular detection of Rickettsia species in ticks collected from the southwestern provinces of the Republic of Korea
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PHRP : Osong Public Health and Research Perspectives