- Impact of Time Delay in Processing Blood Sample on Next Generation Sequencing for Transcriptome Analysis
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Jae-Eun Lee, So-Young Jung, So-Youn Shin, Young-Youl Kim
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Osong Public Health Res Perspect. 2018;9(3):130-132. Published online June 30, 2018
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DOI: https://doi.org/10.24171/j.phrp.2018.9.3.09
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4,650
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Citations
Citations to this article as recorded by  - Effects of processing conditions on stability of immune analytes in human blood
Andres Gottfried-Blackmore, Samuel J. S. Rubin, Lawrence Bai, Sheun Aluko, Yujun Yang, Walter Park, Aida Habtezion
Scientific Reports.2020;[Epub] CrossRef
- Instability of Plasma and Serum Progastrin-Releasing Peptide During Repeated Freezing and Thawing
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Jae-Eun Lee, Jin-Hyun Lee, Maria Hong, Seul-Ki Park, Ji-In Yu, So-Youn Shin, Shine Young Kim
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Osong Public Health Res Perspect. 2016;7(6):351-355. Published online December 31, 2016
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DOI: https://doi.org/10.1016/j.phrp.2016.11.004
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3,434
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 Abstract
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- Objectives
Progastrin-releasing peptide (proGRP) is a promising biomarker for small cell lung cancer. However, not much is known about how sample processing and storage conditions affect the stability of proGRP. Here, we examined the effects of repeated freeze–thaw cycles on the stability of proGRP in plasma and serum.
Methods
Concentrations of proGRP were measured in plasma and serum samples exposed to two, three, or four freeze–thaw cycles and these were compared with values of corresponding samples exposed to one cycle (baseline). We also performed the area under the receiver-operating-characteristic curve (AUC) analysis to determine whether the differences of proGRP concentrations between each paired plasma and serum sample (ΔproGRP) can be used for identifying the samples that have been exposed to multiple freeze–thaw cycles.
Results
Concentrations of proGRP gradually decreased in both plasma and serum samples with increasing numbers of freeze–thaw cycles. Reduction rates of proGRP concentrations were greater in serum than in plasma samples and serum proGRP levels declined with statistical significance (p < 0.001) up to 10.1% after four freeze–thaw cycles. The ΔproGRP measurement showed fair accuracy (AUC = 0.741) for identifying samples that had been through four freeze–thaw cycles. The sensitivity was 82.8% and specificity was 62.1% at an optimal cut-off point of > 4.9.
Conclusion
Our study shows that the stability of circulating proGRP is affected in both plasma and serum samples by repeated freezing and thawing. We also show that ΔproGRP could be used for identifying paired plasma and serum samples subjected to multiple freeze–thaw cycles.
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Citations
Citations to this article as recorded by - Effect of Repeated Freeze–Thaw Cycles on Influenza Virus Antibodies
Alessandro Torelli, Elena Gianchecchi, Martina Monti, Pietro Piu, Irene Barneschi, Carolina Bonifazi, Rosa Coluccio, Luisa Ganfini, Luciano Michele La Magra, Silvia Marconi, Ginevra Marzucchi, Ramona Pace, Laura Palladino, Bernardo Biagi, Emanuele Montomo
Vaccines.2021; 9(3): 267. CrossRef - The influence of different blood samples treatment methods on pro-gastrin-releasing peptide
Huiqin Jiang, Ling Luo, Kang Xiong, Chengwen He, Huaizhou Wang, Yanghua Qin
Medicine.2019; 98(26): e16130. CrossRef
- Inorganic Phosphorus and Potassium Are Putative Indicators of Delayed Separation of Whole Blood
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Jae-Eun Lee, Maria Hong, Seul-Ki Park, Ji-In Yu, Jin-Sook Wang, Haewon Shin, Jong-Wan Kim, Bok-Ghee Han, So-Youn Shin
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Osong Public Health Res Perspect. 2016;7(2):90-95. Published online April 30, 2016
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DOI: https://doi.org/10.1016/j.phrp.2015.11.003
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3,251
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Abstract
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- Objectives
The delayed separation of whole blood can influence the concentrations of circulating blood components, including metabolites and cytokines. The aim of this study was to determine whether clinical-biochemistry analytes can be used to assess the delayed separation of whole blood.
Methods
We investigated the plasma and serum concentrations of five clinical-biochemistry analytes and free hemoglobin when the centrifugation of whole blood stored at 4°C or room temperature was delayed for 4 hours, 6 hours, 24 hours, or 48 hours, and compared the values with those of matched samples that had been centrifuged within 2 hours after whole-blood collection.
Results
The inorganic phosphorus (IP) levels in the plasma and serum samples were elevated ≥ 1.5-fold when whole-blood centrifugation was delayed at room temperature for 48 hours. Furthermore, the IP levels in the plasma samples showed excellent assessment accuracy [area under the receiver-operating-characteristic curve (AUC) > 0.9] after a 48-hour delay in whole-blood separation, and high sensitivity (100%) and specificity (95%) at an optimal cutoff point. The IP levels in the serum samples also exhibited good assessment accuracy (AUC > 0.8), and high sensitivity (81%) and specificity (100%). The potassium (K+) levels were elevated 1.4-fold in the serum samples following a 48-hour delay in whole-blood separation. The K+ levels showed excellent assessment accuracy (AUC > 0.9) following a 48-hour delay in whole-blood separation, and high sensitivity (95%) and specificity (91%) at an optimal cutoff point.
Conclusion
Our study showed that the IP and K+ levels in the plasma or serum samples could be considered as putative indicators to determine whether whole-blood separation had been delayed for extended periods.
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Citations
Citations to this article as recorded by - Assessing the stability of uncentrifuged serum and plasma analytes at various post-collection intervals
Atiqah Mokhsin, Poonaresi Subramaniam, Sivasooriar Sivaneson, Nelson Nheu, Gobhy Ramaloo, Azana S. Hanifah, Sumitha B. Mahathevan, Mohanaraja Nadarajah, Gayathiri Sampasivam, Aletza Mohd Ismail, Thuhairah Abdul Rahman
Journal of Laboratory Medicine.2024;[Epub] CrossRef - Concordance in COVID-19 serology, bone mineralization, and inflammatory analytes between venous and self-collected capillary blood samples exposed to various pre-analytical conditions
Banafshe Hosseini, Harika Dasari, Anna Smyrnova, Claude Bourassa, Jing Leng, Christian Renaud, Francine M Ducharme
Annals of Clinical Biochemistry: International Jou.2023; 60(4): 259. CrossRef - Assessment of avian health status: suitability and constraints of the Zoetis VetScan VS2 blood analyser for ecological and evolutionary studies
Ye Xiong, Michael Tobler, Arne Hegemann, Dennis L. Hasselquist
Biology Open.2023;[Epub] CrossRef - Blood Plasma Quality Control by Plasma Glutathione Status
Tamara Tomin, Natalie Bordag, Elmar Zügner, Abdullah Al-Baghdadi, Maximilian Schinagl, Ruth Birner-Gruenberger, Matthias Schittmayer
Antioxidants.2021; 10(6): 864. CrossRef - Identification of specific pre-analytical quality control markers in plasma and serum samples
Luz Ruiz-Godoy, Virginia Enríquez-Cárcamo, Lourdes Suárez-Roa, María Lourdes Lopez-Castro, Abel Santamaría, Mario Orozco-Morales, Ana Laura Colín-González
Analytical Methods.2019; 11(17): 2259. CrossRef - Proteomics and Lipidomics in Inflammatory Bowel Disease Research: From Mechanistic Insights to Biomarker Identification
Bjoern Titz, Raffaella M. Gadaleta, Giuseppe Lo Sasso, Ashraf Elamin, Kim Ekroos, Nikolai V. Ivanov, Manuel C. Peitsch, Julia Hoeng
International Journal of Molecular Sciences.2018; 19(9): 2775. CrossRef - Impact of Preanalytical Variations in Blood-Derived Biospecimens on Omics Studies: Toward Precision Biobanking?
Jae-Eun Lee, Young-Youl Kim
OMICS: A Journal of Integrative Biology.2017; 21(9): 499. CrossRef - Effect of delayed centrifugation of whole blood on serum samples stability
Massimo Daves, Vincenzo Roccaforte, Michele Giacomi, Monica Riva, Maria Leitner, Stefan Platzgummer, Gertraud Goetsch, Giuseppe Lippi
La Rivista Italiana della Medicina di Laboratorio .2017; 13(1): 41. CrossRef - Instability of Plasma and Serum Progastrin-Releasing Peptide During Repeated Freezing and Thawing
Jae-Eun Lee, Jin-Hyun Lee, Maria Hong, Seul-Ki Park, Ji-In Yu, So-Youn Shin, Shine Young Kim
Osong Public Health and Research Perspectives.2016; 7(6): 351. CrossRef
- Effect of Repeated Freezing and Thawing on Biomarker Stability in Plasma and Serum Samples
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Jae-Eun Lee, Shine Young Kim, So-Youn Shin
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Osong Public Health Res Perspect. 2015;6(6):357-362. Published online December 31, 2015
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DOI: https://doi.org/10.1016/j.phrp.2015.11.005
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3,679
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28
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45
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Abstract
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- Objectives
The stability of circulating proteins can be affected by repeated freezing and thawing. The aim of our study was to identify the effect of repeated freezing and thawing on the plasma and serum concentrations of eight proteins [interferon-γ, interleukin (IL)-8, IL-15, IL-17A, matrix metalloproteinase (MMP)-7, tumor necrosis factor-α, vascular endothelial growth factor (VEGF), and VEGF receptor 2 (VEGF-R2)].
Methods
We assessed the concentration changes of these proteins in 30 plasma and serum samples subjected to three, four, or five freeze–thaw cycles, and compared these with the concentration changes in the samples that were subjected to two freeze–thaw cycles before analysis.
Results
Repeated freezing and thawing by up to five cycles did not modify the plasma and serum concentrations of interferon-γ, IL-8, and VEGF-R2, while levels of MMP-7, tumor necrosis factor-α, and VEGF were significantly changed in both plasma and serum samples. Moreover, MMP-7 and VEGF concentrations tended to increase with freeze–thaw cycles. They were more elevated in plasma samples (up to about 15%) than in serum samples (up to about 7%), suggesting that serum is the preferred sample type for the analysis of circulating proteins.
Conclusion
This is the first report on the effect of repeated freezing and thawing on plasma concentrations of MMP-7 and VEGF-R2. Our findings propose that researchers should consider the number of freeze–thaw cycles to select plasma or serum samples, depending on the type of analyte.
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Citations
Citations to this article as recorded by - Reliability, stability during long-term storage, and intra-individual variation of circulating levels of osteopontin, osteoprotegerin, vascular endothelial growth factor-A, and interleukin-17A
Tomoki Nakamizo, John Cologne, Takeshi Kishi, Tetsuya Takahashi, Mayumi Inoue, Hiroyuki Ryukaku, Tomonori Hayashi, Yoichiro Kusunoki, Saeko Fujiwara, Waka Ohishi
European Journal of Medical Research.2024;[Epub] CrossRef - Quantitative and qualitative analysis of stability for 16 serum immunoregulators over 50 freeze–thaw cycles
R. N. Chowdhury, A. Armato, E. Culver, L. Shteynman, C. Kurien, B. Cradin, F. Margolin, T. Nguyen, C. Cardona, N. Kabir, R. M. Garruto, J. K. Lum, K. Wander
American Journal of Human Biology.2024;[Epub] CrossRef - Dynamics of serum levels of TNF-α in a longitudinal follow-up study in 98 patients with juvenile idiopathic arthritis treated with anti-TNF-α biological drugs
N. Morozova, M. Zajc Avramovič, G. Markelj, N. Toplak, T. Avčin
Clinical Rheumatology.2024; 43(7): 2287. CrossRef - Sample processing time but not storage time affects complement activation markers C4a, C4d, C3a, iC3b, Bb, C5a, and sC5b-9 levels in EDTA-plasma of individuals at clinical high-risk for psychosis
Eleftheria Kodosaki, Colm Healy, Jonah F. Byrne, Melanie Föcking, Mary Cannon, Diana O. Perkins, David Cotter, Meike Heurich
Biomarkers in Neuropsychiatry.2024; 10: 100097. CrossRef - Biobanking for Neurodegenerative Diseases: Challenge for Translational Research and Data Privacy
Emilia Giannella, Valentino Notarangelo, Caterina Motta, Giulia Sancesario
The Neuroscientist.2023; 29(2): 190. CrossRef - Keep cool! Observed temperature variations at different process stages of the biobanking workflow – examples from the Leipzig medical biobank
Juliane Weikert, Angelina Mehrländer, Ronny Baber
Journal of Laboratory Medicine.2023; 47(2): 69. CrossRef - The Stability of the Anti-Müllerian Hormone in Serum and Plasma Samples under Various Preanalytical Conditions
Radana Vrzáková, Václav Šimánek, Ondřej Topolčan, Vladimír Vurm, David Slouka, Radek Kučera
Diagnostics.2023; 13(8): 1501. CrossRef - Differences in the inflammatory proteome of East African and Western European adults and associations with environmental and dietary factors
Godfrey S Temba, Nadira Vadaq, Vesla Kullaya, Tal Pecht, Paolo Lionetti, Duccio Cavalieri, Joachim L Schultze, Reginald Kavishe, Leo AB Joosten, Andre J van der Ven, Blandina T Mmbaga, Mihai G Netea, Quirijn de Mast
eLife.2023;[Epub] CrossRef - Influence of Freezing Temperature, Freezing Duration, and Repeated Freeze/Thaw Cycles on Electrophoretic Profiles in the White Stork (Ciconia ciconia)
Milan Thorel, Yannick Roman, Antoine Leclerc
Journal of Avian Medicine and Surgery.2023;[Epub] CrossRef - Clinical Spectroscopy: Lost in Translation?
James M. Cameron, Christopher Rinaldi, Samantha H. Rutherford, Alexandra Sala, Ashton G. Theakstone, Matthew J. Baker
Applied Spectroscopy.2022; 76(4): 393. CrossRef - Correlation of Repeat Measurements of 27 Candidate Protein Markers for Colorectal Cancer Screening Taken Three Years and Multiple Freeze–Thaw Cycles Apart
Megha Bhardwaj, Petra Schrotz-King, Hermann Brenner
Life.2022; 12(3): 359. CrossRef - Development of quinone linked immunosorbent assay (QuLISA) based on using Folin’s reagent as a non-enzymatic tag: Application to analysis of food allergens
Mahmoud El-Maghrabey, Yudai Sato, Fatema Kaladari, Naoya Kishikawa, Naotaka Kuroda
Sensors and Actuators B: Chemical.2022; 368: 132167. CrossRef - A systematic review and meta-analysis of preanalytical factors and methodological differences influencing the measurement of circulating vascular endothelial growth factor
Ulrika Sjöbom, Anders K. Nilsson, Hanna Gyllensten, Ann Hellström, Chatarina Löfqvist, Angela J. Glading
PLOS ONE.2022; 17(7): e0270232. CrossRef - Cytokines: From Clinical Significance to Quantification
Chao Liu, Dewei Chu, Kourosh Kalantar‐Zadeh, Jacob George, Howard A. Young, Guozhen Liu
Advanced Science.2021;[Epub] CrossRef - Microheterogeneity and preanalytical stability of protein biomarkers of inflammation and renal function
Jie Gao, Arve Ulvik, Adrian McCann, Per Magne Ueland, Klaus Meyer
Talanta.2021; 223: 121774. CrossRef - The effect of pre-analytical handling on the stability of fractalkine, monocyte chemoattractant protein 1 (MCP1), interleukin 6 and interleukin 8 in samples of human cerebrospinal fluid
Winnie Charlotte Pedersen Mortensen, Laila Bendix, Hanne Irene Jensen, Claus Varnum, Lasse Enkebølle Rasmussen, Jørgen T. Lauridsen, Nis Borbye-Lorenzen, Kristin Skogstrand, Palle Toft, Henrik Bjarke Vaegter, Morten Rune Blichfeldt-Eckhardt
Journal of Immunological Methods.2021; 494: 113057. CrossRef - Standardizing salivary lactoferrin measurements to obtain a robust diagnostic biomarker for Alzheimer's disease
Fernando Bartolome, Gorka Orive, Eva Carro
Alzheimer's & Dementia: Diagnosis, Assessment & Di.2021;[Epub] CrossRef - Pre-analytical considerations in biomarker research: focus on cardiovascular disease
Elena Revuelta-López, Jaume Barallat, Adriana Cserkóová, Carolina Gálvez-Montón, Allan S. Jaffe, James L. Januzzi, Antoni Bayes-Genis
Clinical Chemistry and Laboratory Medicine (CCLM).2021; 59(11): 1747. CrossRef - Vaginal cytokine profile and microbiota before and after lubricant use compared with condomless vaginal sex: a preliminary observational study
Susan Tuddenham, Christina A. Stennett, Richard A. Cone, Jacques Ravel, Andrew N. Macintyre, Khalil G. Ghanem, Xin He, Rebecca M. Brotman
BMC Infectious Diseases.2021;[Epub] CrossRef - Proximity Extension Assay in Combination with Next-Generation Sequencing for High-throughput Proteome-wide Analysis
Lotta Wik, Niklas Nordberg, John Broberg, Johan Björkesten, Erika Assarsson, Sara Henriksson, Ida Grundberg, Erik Pettersson, Christina Westerberg, Elin Liljeroth, Adam Falck, Martin Lundberg
Molecular & Cellular Proteomics.2021; 20: 100168. CrossRef - Serum or plasma? An old question looking for new answers
Mario Plebani, Giuseppe Banfi, Sergio Bernardini, Francesco Bondanini, Laura Conti, Romolo Dorizzi, Fulvio Enrico Ferrara, Rita Mancini, Tommaso Trenti
Clinical Chemistry and Laboratory Medicine (CCLM).2020; 58(2): 178. CrossRef - Association Between Intravenous Fluid Bolus and Biomarker Trajectory During Prehospital Care
Emily B. Brant, Jason Kennedy, Christian Martin-Gill, Vanessa Jackson, Octavia M. Peck Palmer, Clifton W. Callaway, Jeremy M. Kahn, Donald M. Yealy, Derek C. Angus, Christopher W. Seymour
Prehospital Emergency Care.2020; 24(2): 196. CrossRef - Evaluating sample stability in the clinical laboratory with the help of linear and non-linear regression analysis
Joachim K.W. Pum
Clinical Chemistry and Laboratory Medicine (CCLM).2020; 58(2): 188. CrossRef - Optimization of Saliva Collection and Immunochromatographic Detection of Salivary Pepsin for Point-of-Care Testing of Laryngopharyngeal Reflux
Young Ju Lee, Jiyoon Kwon, Sanggyeong Shin, Young-Gyu Eun, Jae Ho Shin, Gi-Ja Lee
Sensors.2020; 20(1): 325. CrossRef - Stability of 12 T-helper cell-associated cytokines in human serum under different pre-analytical conditions
Ulrik Stervbo, Sharon Bajda, Patrizia Wehler, Benjamin J. Rohn, Melanie Streichhahn, Sehriban Temizsoy, Eva Kohut, Toralf Roch, Richard Viebahn, Timm H. Westhoff, Nina Babel
Cytokine.2020; 129: 155044. CrossRef - Quinone-based antibody labeling reagent for enzyme-free chemiluminescent immunoassays. Application to avidin and biotinylated anti-rabbit IgG labeling
Mahmoud El-Maghrabey, Naoya Kishikawa, Shiori Harada, Kaname Ohyama, Naotaka Kuroda
Biosensors and Bioelectronics.2020; 160: 112215. CrossRef - Standardized approaches for clinical sampling and endpoint ascertainment in tuberculous meningitis studies
Ursula K Rohlwink, Felicia C Chow, Sean Wasserman, Sofiati Dian, Rachel PJ Lai, Lidya Chaidir, Raph L Hamers, Robert J Wilkinson, David R Boulware, Fiona V Cresswell, Arjan van Laarhoven
Wellcome Open Research.2020; 4: 204. CrossRef - Exploring pre-analytical factors for the optimisation of serum diagnostics: Progressing the clinical utility of ATR-FTIR spectroscopy
James M. Cameron, Holly J. Butler, David J. Anderson, Loren Christie, Lily Confield, Katie E. Spalding, Duncan Finlayson, Stuart Murray, Zanib Panni, Christopher Rinaldi, Alexandra Sala, Ashton G. Theakstone, Matthew J. Baker
Vibrational Spectroscopy.2020; 109: 103092. CrossRef - QuantiFERON-TB Gold Plus with Chemiluminescence Immunoassay: Do We Need a Higher Cutoff?
Francesco Bisognin, Giulia Lombardi, Maria Carla Re, Paola Dal Monte, Geoffrey A. Land
Journal of Clinical Microbiology.2020;[Epub] CrossRef - Stability of serum biochemical markers during standard long-term storage and with a single thawing
V. A. Kozlova, V. A. Metelskaya, M. S. Pokrovskaya, I. A. Efimova, O. A. Litinskaya, V. A. Kutsenko, E. B. Yarovaya, S. A. Shalnova, O. M. Drapkina
Cardiovascular Therapy and Prevention.2020; 19(6): 2736. CrossRef - Development and application of a porcine specific ELISA for the quantification of soluble CD163
J. Alex Pasternak, Daniel J. MacPhee, John C.S. Harding
Veterinary Immunology and Immunopathology.2019; 210: 60. CrossRef - Dynamics of circulating TNF during adalimumab treatment using a drug-tolerant TNF assay
Lea C. Berkhout, Merel J. l’Ami, Jill Ruwaard, Margreet H. Hart, Pleuni Ooijevaar-de Heer, Karien Bloem, Michael T. Nurmohamed, Ronald F. van Vollenhoven, Maarten Boers, Daniel F. Alvarez, Catherine H. Smith, Gerrit J. Wolbink, Theo Rispens
Science Translational Medicine.2019;[Epub] CrossRef - Standardized approaches for clinical sampling and endpoint ascertainment in tuberculous meningitis studies
Ursula K Rohlwink, Felicia C Chow, Sean Wasserman, Sofiati Dian, Rachel PJ Lai, Lidya Chaidir, Raph L Hamers, Robert J Wilkinson, David R Boulware, Fiona V Cresswell, Arjan van Laarhoven
Wellcome Open Research.2019; 4: 204. CrossRef - The Effect of Anticoagulants, Temperature, and Time on the Human Plasma Metabolome and Lipidome from Healthy Donors as Determined by Liquid Chromatography-Mass Spectrometry
Manoj Khadka, Andrei Todor, Kristal M. Maner-Smith, Jennifer K. Colucci, ViLinh Tran, David A. Gaul, Evan J. Anderson, Muktha S. Natrajan, Nadine Rouphael, Mark J. Mulligan, Circe E. McDonald, Mehul Suthar, Shuzhao Li, Eric A. Ortlund
Biomolecules.2019; 9(5): 200. CrossRef - A caspase-6-cleaved fragment of Glial Fibrillary Acidic Protein as a potential serological biomarker of CNS injury after cardiac arrest
Ditte S. Jonesco, Christian Hassager, Martin Frydland, Jesper Kjærgaard, Morten Karsdal, Kim Henriksen, Stephen D. Ginsberg
PLOS ONE.2019; 14(11): e0224633. CrossRef - Angiogenic Factor and Cytokine Analysis among Patients Treated with Adjuvant VEGFR TKIs in Resected Renal Cell Carcinoma
Wenxin Xu, Maneka Puligandla, Judith Manola, Andrea J. Bullock, Daniel Tamasauskas, David F. McDermott, Michael B. Atkins, Naomi B. Haas, Keith Flaherty, Robert G. Uzzo, Janice P. Dutcher, Robert S. DiPaola, Rupal S. Bhatt
Clinical Cancer Research.2019; 25(20): 6098. CrossRef - Stability of important antibodies for kidney disease: pre-analytic methodological considerations
Qiuxia Han, Songyan Li, Bo Fu, Dongwei Liu, Maoqing Wu, Xiaoli Yang, Guangyan Cai, Zhangsuo Liu, Xiangmei Chen, Hanyu Zhu
PeerJ.2018; 6: e5178. CrossRef - The association of angiogenic factors and chronic kidney disease
Christopher E. Anderson, L. Lee Hamm, Gem Batuman, Damodar R. Kumbala, Chung-Shiuan Chen, Swapna G. Kallu, Ravi Siriki, Shilpa Gadde, Myra A. Kleinpeter, N. Kevin Krane, Eric E. Simon, Jiang He, Jing Chen
BMC Nephrology.2018;[Epub] CrossRef - Strong impact on plasma protein profiles by precentrifugation delay but not by repeated freeze-thaw cycles, as analyzed using multiplex proximity extension assays
Qiujin Shen, Johan Björkesten, Joakim Galli, Daniel Ekman, John Broberg, Niklas Nordberg, Annika Tillander, Masood Kamali-Moghaddam, Gunnel Tybring, Ulf Landegren
Clinical Chemistry and Laboratory Medicine (CCLM).2018; 56(4): 582. CrossRef - Plasma-Derived Inflammatory Proteins Predict Oral Squamous Cell Carcinoma
Kelly Yi Ping Liu, Xian Jun David Lu, Yuqi Sarah Zhu, Nhu Le, Hugh Kim, Catherine F. Poh
Frontiers in Oncology.2018;[Epub] CrossRef - Is vascular endothelial growth factor a useful biomarker in giant cell arteritis?
Nicola Goodfellow, Julien Morlet, Surjeet Singh, Afsie Sabokbar, Andrew Hutchings, Vanshika Sharma, Jana Vaskova, Shauna Masters, Allahdad Zarei, Raashid Luqmani
RMD Open.2017; 3(1): e000353. CrossRef - Indices of immune function used by ecologists are mostly unaffected by repeated freeze-thaw cycles and methodological deviations
Arne Hegemann, Sara Pardal, Kevin D. Matson
Frontiers in Zoology.2017;[Epub] CrossRef - The Enhanced Liver Fibrosis (ELF) Panel: Analyte Stability Under Common Sample Storage Conditions Used in Clinical Practice
Oliver J Kennedy, Julie Parkes, Sudeep Tanwar, Paul M Trembling, William M Rosenberg
The Journal of Applied Laboratory Medicine.2017; 1(6): 720. CrossRef - Instability of Plasma and Serum Progastrin-Releasing Peptide During Repeated Freezing and Thawing
Jae-Eun Lee, Jin-Hyun Lee, Maria Hong, Seul-Ki Park, Ji-In Yu, So-Youn Shin, Shine Young Kim
Osong Public Health and Research Perspectives.2016; 7(6): 351. CrossRef - Effects of Long-Term Storage Time and Original Sampling Month on Biobank Plasma Protein Concentrations
Stefan Enroth, Göran Hallmans, Kjell Grankvist, Ulf Gyllensten
EBioMedicine.2016; 12: 309. CrossRef
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