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Won-Ja Lee 10 Articles
One-Step Reverse Transcription-Polymerase Chain Reaction for Ebola and Marburg Viruses
Sun-Whan Park, Ye-Ji Lee, Won-Ja Lee, Youngmee Jee, WooYoung Choi
Osong Public Health Res Perspect. 2016;7(3):205-209.   Published online June 30, 2016
DOI: https://doi.org/10.1016/j.phrp.2016.04.004
  • 3,283 View
  • 26 Download
  • 5 Crossref
AbstractAbstract PDF
Objectives
Ebola and Marburg viruses (EBOVs and MARVs, respectively) are causative agents of severe hemorrhagic fever with high mortality rates in humans and nonhuman primates. In 2014, there was a major Ebola outbreak in various countries in West Africa, including Guinea, Liberia, Republic of Sierra Leone, and Nigeria. EBOV and MARV are clinically difficult to diagnose and distinguish from other African epidemic diseases. Therefore, in this study, we aimed to develop a method for rapid identification of the virus to prevent the spread of infection.
Methods
We established a conventional one-step reverse transcription-polymerase chain reaction (RT-PCR) assay for these pathogens based on the Superscript Reverse Transcriptase-Platinum Taq polymerase enzyme mixture. All assays were thoroughly optimized using in vitro-transcribed RNA.
Results
We designed seven primer sets of nucleocapsid protein (NP) genes based on sequences from seven filoviruses, including five EBOVs and two MARVs. To evaluate the sensitivity of the RT-PCR assay for each filovirus, 10-fold serial dilutions of synthetic viral RNA transcripts of EBOV or MARV NP genes were used to assess detection limits of viral RNA copies. The potential for these primers to cross react with other filoviruses was also examined. The results showed that the primers were specific for individual genotype detection in the examined filoviruses.
Conclusion
The assay established in this study may facilitate rapid, reliable laboratory diagnosis in suspected cases of Ebola and Marburg hemorrhagic fevers.

Citations

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  • Marburg Virus Disease – A Mini-Review
    Sandip Chakraborty, Deepak Chandran, Ranjan K. Mohapatra, Mahmoud Alagawany, Mohd Iqbal Yatoo, Md. Aminul Islam, Anil K. Sharma, Kuldeep Dhama
    Journal of Experimental Biology and Agricultural S.2022; 10(4): 689.     CrossRef
  • Marburgviruses: An Update
    Caterina M Miraglia
    Laboratory Medicine.2019; 50(1): 16.     CrossRef
  • Ebola virus: A global public health menace: A narrative review
    Shamimul Hasan, SyedAnsar Ahmad, Rahnuma Masood, Shazina Saeed
    Journal of Family Medicine and Primary Care.2019; 8(7): 2189.     CrossRef
  • Fast and Parallel Detection of Four Ebola Virus Species on a Microfluidic-Chip-Based Portable Reverse Transcription Loop-Mediated Isothermal Amplification System
    Xue Lin, Xiangyu Jin, Bin Xu, Ruliang Wang, Rongxin Fu, Ya Su, Kai Jiang, Han Yang, Ying Lu, Yong Guo, Guoliang Huang
    Micromachines.2019; 10(11): 777.     CrossRef
  • The current landscape of nucleic acid tests for filovirus detection
    David J. Clark, John Tyson, Andrew D. Sails, Sanjeev Krishna, Henry M. Staines
    Journal of Clinical Virology.2018; 103: 27.     CrossRef
Comparison of the Epidemiological Aspects of Imported Dengue Cases between Korea and Japan, 2006–2010
Young Eui Jeong, Won-Chang Lee, Jung Eun Cho, Myung-Guk Han, Won-Ja Lee
Osong Public Health Res Perspect. 2016;7(1):71-74.   Published online February 28, 2016
DOI: https://doi.org/10.1016/j.phrp.2015.12.001
  • 3,400 View
  • 21 Download
  • 12 Crossref
AbstractAbstract PDF
To compare the epidemiological characteristics of dengue cases imported by travelers or immigration in both Korea and Japan, we determined dengue incidence and related risk factors. During 2006–2010, 367 and 589 imported dengue cases were reported in Korea and Japan, respectively. In Korea, the presumptive origins for the dengue infections were Southeast Asia (82.6%), Southern Asia (13.9%), Eastern Asia (1.1%), South America (0.3%), Central America (0.3%), Africa (0.3%), and other countries (1.6%). In Japan, the origins of the infections were Southeast Asia (69.8%), Southern Asia (20.0%), Eastern Asia (1.7%), South America (2.5%), Central America (1.2%), Africa (1.2%), Oceania (2.4%), and other countries (1.2%). In both countries, more dengue cases were reported for men than for women (p < 0.01), and those aged 20–30 years accounted for > 60% of the total cases. The frequency of imported cases in summer and autumn (∼70% of total cases) was similar in both countries. This study demonstrates that there is a similar pattern of imported dengue cases in Korea and Japan. Therefore, there is a risk of an autochthonous dengue outbreak in Korea, as indicated by the recent outbreak in Japan in 2014.

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  • Molecular and Haematological Analysis of Dengue Virus-3 Among Children in Lahore, Pakistan
    Muhammad Kashif, Muhammad Afzal, Basit Zeshan, Hasnain Javed, Salma Batool, Modasrah Mazhar
    Jundishapur Journal of Microbiology.2021;[Epub]     CrossRef
  • Molecular evolution of dengue virus types 1 and 4 in Korean travelers
    Eun-Ha Hwang, Green Kim, Hoyin Chung, Hanseul Oh, Jong-Hwan Park, Gyeung Haeng Hur, JungJoo Hong, Bon-Sang Koo
    Archives of Virology.2021; 166(4): 1103.     CrossRef
  • Aedes albopictus and Aedes flavopictus (Diptera: Culicidae) pre-imaginal abundance patterns are associated with different environmental factors along an altitudinal gradient
    Luis Fernando Chaves, Mariel D. Friberg
    Current Research in Insect Science.2021; 1: 100001.     CrossRef
  • Evolution, heterogeneity and global dispersal of cosmopolitan genotype of Dengue virus type 2
    Surya Pavan Yenamandra, Carmen Koo, Suzanna Chiang, Han Shi Jeri Lim, Zhen Yuan Yeo, Lee Ching Ng, Hapuarachchige Chanditha Hapuarachchi
    Scientific Reports.2021;[Epub]     CrossRef
  • Temporal Trend ofAedes albopictusin Local Urban Parks of the Republic of Korea
    Myung-Jae Hwang, Jong-Hun Kim, Heung-Chul Kim, Myung Soon Kim, Terry A Klein, Juhwa Choi, Kisung Sim, Yeonseung Chung, Yadav Prasad Joshi, Hae-Kwan Cheong, Kristen Healy
    Journal of Medical Entomology.2020; 57(4): 1082.     CrossRef
  • A Two-Patch Mathematical Model for Temperature-Dependent Dengue Transmission Dynamics
    Jung Kim, Yongin Choi, James Kim, Sunmi Lee, Chang Lee
    Processes.2020; 8(7): 781.     CrossRef
  • Potential effects of climate change on dengue transmission dynamics in Korea
    Hyojung Lee, Jung Eun Kim, Sunmi Lee, Chang Hyeong Lee, Shamala Devi Sekaran
    PLOS ONE.2018; 13(6): e0199205.     CrossRef
  • Analysis of Dengue Virus Burden and Serotypes Pattern in Faisalabad, 2016–2017
    Muhammad Yousaf, Kashaf Junaid, Muhammad Sarfaraz Iqbal, Imran Aslam, Sheraz Ahmad, Muhammad Aqeel, Usman Ali Ashfaq, Saba Khaliq, Muhammad Usman Ghani, Nayyar Waqar
    Future Virology.2018; 13(4): 245.     CrossRef
  • Seroprevalence of Toxoplasmosis with ELISA and Rapid Diagnostic Test among Residents in Gyodong-do, Inchon city, Korea: A Four-Year Follow-up
    Yeong Hoon Kim, Ji hoo Lee, Seong kyu Ahn, Tong-Soo Kim, Sung-Jong Hong, Chom-Kyu Chong, Hye-Jin Ahn, Ho-Woo Nam
    The Korean Journal of Parasitology.2017; 55(3): 247.     CrossRef
  • A Disease Around the Corner
    Hae-Wol Cho, Chaeshin Chu
    Osong Public Health and Research Perspectives.2016; 7(1): 1.     CrossRef
  • WITHDRAWN: A disease around the corner
    Hae-Wol Cho, Chaeshin Chu
    Osong Public Health and Research Perspectives.2016;[Epub]     CrossRef
  • Prospects for dengue vaccines for travelers
    Sl-Ki Lim, Yong Seok Lee, Suk Namkung, Jacqueline K Lim, In-Kyu Yoon
    Clinical and Experimental Vaccine Research.2016; 5(2): 89.     CrossRef
Ebola Hemorrhagic Fever and the Current State of Vaccine Development
Joo Eun Hong, Kee-Jong Hong, Woo Young Choi, Won-Ja Lee, Yeon Hwa Choi, Chung-Hyeon Jeong, Kwang-il Cho
Osong Public Health Res Perspect. 2014;5(6):378-382.   Published online December 31, 2014
DOI: https://doi.org/10.1016/j.phrp.2014.09.006
  • 3,858 View
  • 19 Download
  • 5 Crossref
AbstractAbstract PDF
Current Ebola virus outbreak in West Africa already reached the total number of 1,323 including 729 deaths by July 31st. the fatality is around 55% in the southeastern area of Guinea, Sierra Leone, Liberia, and Nigeria. The number of patients with Ebola Hemorrhagic Fever (EHF) was continuously increasing even though the any effective therapeutics or vaccines has not been developed yet. The Ebola virus in Guinea showed 98% homology with Zaire Ebola Virus.Study of the pathogenesis of Ebola virus infection and assess of the various candidates of vaccine have been tried for a long time, especially in United States and some European countries. Even though the attenuated live vaccine and DNA vaccine containing Ebola viral genes were tested and showed efficacy in chimpanzees, those candidates still need clinical tests requiring much longer time than the preclinical development to be approved for the practical treatment.It can be expected to eradicate Ebola virus by a safe and efficient vaccine development similar to the case of smallpox virus which was extinguished from the world by the variola vaccine.

Citations

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  • Recent developments and strategies of Ebola virus vaccines
    Ashish Ranjan Sharma, Yeon-Hee Lee, Sudarshini Nath, Sang-Soo Lee
    Current Opinion in Pharmacology.2021; 60: 46.     CrossRef
  • Predictive Effects of Novelty Measured by Temporal Embeddings on the Growth of Scientific Literature
    Jiangen He, Chaomei Chen
    Frontiers in Research Metrics and Analytics.2018;[Epub]     CrossRef
  • Social network analysis and modeling of cellphone-based syndromic surveillance data for Ebola in Sierra Leone
    Jia B. Kangbai
    Asian Pacific Journal of Tropical Medicine.2016; 9(9): 851.     CrossRef
  • El desarrollo de nuevas vacunas
    Fernando González-Romo, Juan J. Picazo
    Enfermedades Infecciosas y Microbiología Clínica.2015; 33(8): 557.     CrossRef
  • Out of Africa, Into Global Health Security Agenda
    Hae-Wol Cho, Chaeshin Chu
    Osong Public Health and Research Perspectives.2014; 5(6): 313.     CrossRef
Cloning and Expression of Recombinant Tick-Borne Encephalitis Virus-like Particles in Pichia pastoris
Seok-Min Yun, Young Eui Jeong, Eunbyeol Wang, Ye-Ji Lee, Myung Guk Han, Chan Park, Won-Ja Lee, WooYoung Choi
Osong Public Health Res Perspect. 2014;5(5):274-278.   Published online October 31, 2014
DOI: https://doi.org/10.1016/j.phrp.2014.08.005
  • 3,704 View
  • 20 Download
  • 10 Crossref
AbstractAbstract PDF
Objectives
The purpose of this study was to verify the feasibility of using the glyceraldehyde-3-phosphate dehydrogenase (GAP) promotor based Pichia pastoris expression system to produce tick-borne encephalitis virus (TBEV) virus-like particles (VLPs).
Methods
The complementary DNA encoding the TBEV prM signal peptide, prM, and E proteins of TBEV Korean strain (KrM 93) was cloned into the plasmid vector pGAPZɑA, then integrated into the genome of P. pastoris, under the control of the GAP promoter. Expression of TBEV VLPs was determined by Western blotting using monoclonal antibody against TBEV envelope (E) protein.
Results
Recombinant TBEV VLPs consisting of prM and E protein were successfully expressed using the GAP promoter-based P. pastoris expression system. The results of Western blotting showed that the recombinant proteins were secreted into the culture supernatant from the P. pastoris and glycosylated.
Conclusion
This study suggests that recombinant TBEV VLPs from P. pastoris offer a promising approach to the production of VLPs for use as vaccines and diagnostic antigens.

Citations

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  • Influence of adjuvant type and route of administration on the immunogenicity of Leishmania-derived tick-borne encephalitis virus-like particles – A recombinant vaccine candidate
    Marta Zimna, Gabriela Brzuska, Jiří Salát, Daniel Růžek, Ewelina Krol
    Antiviral Research.2024; 228: 105941.     CrossRef
  • Yeast-Based Virus-like Particles as an Emerging Platform for Vaccine Development and Delivery
    Vartika Srivastava, Kripa N. Nand, Aijaz Ahmad, Ravinder Kumar
    Vaccines.2023; 11(2): 479.     CrossRef
  • Yeast and Virus-like Particles: A Perfect or Imperfect Couple?
    Sara Brachelente, Alvaro Galli, Tiziana Cervelli
    Applied Microbiology.2023; 3(3): 805.     CrossRef
  • De novo transcriptome sequencing and comparative profiling of the ovary in partially engorged and fully engorged Haemaphysalis flava ticks
    Yu Zhao, Zhe-Hui Qu, Feng-Chao Jiao
    Parasitology International.2021; 83: 102344.     CrossRef
  • Flavivirus vaccines: Virus-like particles and single-round infectious particles as promising alternatives
    Esmeralda Cuevas-Juárez, Victoria Pando-Robles, Laura A. Palomares
    Vaccine.2021; 39(48): 6990.     CrossRef
  • NS1 Recombinant Proteins Are Efficiently Produced in Pichia pastoris and Have Great Potential for Use in Diagnostic Kits for Dengue Virus Infections
    Mariana Fonseca Xisto, John Willians Oliveira Prates, Ingrid Marques Dias, Roberto Sousa Dias, Cynthia Canedo da Silva, Sérgio Oliveira de Paula
    Diagnostics.2020; 10(6): 379.     CrossRef
  • Tick-Borne Encephalitis Virus: A Quest for Better Vaccines against a Virus on the Rise
    Mareike Kubinski, Jana Beicht, Thomas Gerlach, Asisa Volz, Gerd Sutter, Guus F. Rimmelzwaan
    Vaccines.2020; 8(3): 451.     CrossRef
  • Virus-Like Particle Systems for Vaccine Development against Viruses in the Flaviviridae Family
    Shu Hui Wong, Alagie Jassey, Jonathan Y. Wang, Wei-Cheng Wang, Ching-Hsuan Liu, Liang-Tzung Lin
    Vaccines.2019; 7(4): 123.     CrossRef
  • ON MODERN APPROACHES TO CREATION OF A SINGLE-CYCLE VACCINE AGAINST TICK-BORNE ENCEPHALITIS
    V. A. Lashkevich, G. G. Karganova
    Problems of Virology.2018; 63(3): 101.     CrossRef
  • Production of an enzymatically active and immunogenic form of ectodomain of Porcine rubulavirus hemagglutinin-neuraminidase in the yeast Pichia pastoris
    José Luis Cerriteño-Sánchez, Gerardo Santos-López, Nora Hilda Rosas-Murrieta, Julio Reyes-Leyva, Sandra Cuevas-Romero, Irma Herrera-Camacho
    Journal of Biotechnology.2016; 223: 52.     CrossRef
Comparison of Four Serological Tests for Detecting Antibodies to Japanese Encephalitis Virus after Vaccination in Children
Go Woon Cha, Jung Eun Cho, Young Ran Ju, Young-Jin Hong, Myung Guk Han, Won-Ja Lee, Eui Yul Choi, Young Eui Jeong
Osong Public Health Res Perspect. 2014;5(5):286-291.   Published online October 31, 2014
DOI: https://doi.org/10.1016/j.phrp.2014.08.003
  • 3,727 View
  • 25 Download
  • 16 Crossref
AbstractAbstract PDF
Objectives
Several different methods are currently used to detect antibodies to Japanese encephalitis virus (JEV) in serum samples or cerebrospinal fluid. These methods include the plaque reduction neutralization test (PRNT), the hemagglutination inhibition (HI) test, indirect immunofluorescence assay (IFA), and enzyme-linked immunosorbent assay (ELISA). The purpose of this study was to compare the performance of each method in detecting vaccine-induced antibodies to JEV.
Methods
The study included 29 children who had completed a primary immunization schedule with an inactivated vaccine against JEV derived from mouse brain (n = 15) or a live attenuated SA14-14-2 vaccine (n = 14). Serum samples were collected between 3 months and 47 months after the last immunization. The serum samples were tested by performing the PRNT, HI test, in-house IFA, and commercial ELISA. The antibody detection rates were compared between tests.
Results
All 29 serum samples were positive with the PRNT, showing antibody titers from 1:20 to 1:2560. The HI test showed positive rates of 86.7% (13/15) and 71.4% (10/14) in the inactivated and live attenuated vaccine groups, respectively. The results of the IFA for immunoglobulin (Ig)G were positive in 53.3% (8/15) of children in the inactivated vaccine group and 35.7% (5/14) in the live attenuated vaccine group. Neither the IFA nor ELISA detected JEV IgM antibodies in any of the 29 children.
Conclusion
These results show that detection rates of vaccine-induced antibodies to JEV have a wide range (0–100%) depending on the testing method as well as the time since immunization and individual differences between children. These findings are helpful in interpreting serological test results for the diagnosis of Japanese encephalitis in situations where vaccines are widely administered.

Citations

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  • Development of Colloidal Gold-Based Immunochromatographic Strips for Rapid Detection and Surveillance of Japanese Encephalitis Virus in Dogs across Shanghai, China
    Dengke Zhong, Abdul Wahaab, Jiayang Zheng, Junjie Zhang, Zhiyong Ma, Jianchao Wei
    Viruses.2024; 16(2): 258.     CrossRef
  • Laboratory evaluation of ELISA and indirect immunofluorescence assay in response to emergence of Japanese encephalitis virus genotype IV in Australia
    Paul Kinsella, Michael Moso, Genevieve Martin, Theo Karapangiotidis, Di Karamalakis, Suellen Nicholson, Mitch Batty, Kathy Jackson, Madeleine Marsland, Tilda Thomson, Lakshmi Manoharan, Helen O'brien, N. Deborah Friedman, Katherine Bond, Deborah A. Willia
    Journal of Clinical Virology.2023; 168: 105580.     CrossRef
  • A review on Japanese Encephalitis virus emergence, pathogenesis and detection: From conventional diagnostics to emerging rapid detection techniques
    Fatima Mohsin, Shariq Suleman, Nigar Anzar, Jagriti Narang, Shikha Wadhwa
    International Journal of Biological Macromolecules.2022; 217: 435.     CrossRef
  • Recent pharmaceutical engineered trends as theranostics for Japanese encephalitis
    Akshada Mhaske, Sanjiv Singh, Mohammed A.S. Abourehab, Akhilesh Kumar, Prashant Kesharwani, Rahul Shukla
    Process Biochemistry.2022; 122: 115.     CrossRef
  • Immunogenicity of a single fractional intradermal dose of Japanese encephalitis live attenuated chimeric vaccine
    Luis Furuya-Kanamori, Narayan Gyawali, Deborah J Mills, Christine Mills, Leon E Hugo, Gregor J Devine, Colleen L Lau
    Journal of Travel Medicine.2022;[Epub]     CrossRef
  • In silico molecular docking in DNA aptamer development
    Tholasi Nadhan Navien, Ramesh Thevendran, Hazrina Yusof Hamdani, Thean-Hock Tang, Marimuthu Citartan
    Biochimie.2021; 180: 54.     CrossRef
  • Pathobiology of Japanese encephalitis virus infection
    Kiran Bala Sharma, Sudhanshu Vrati, Manjula Kalia
    Molecular Aspects of Medicine.2021; 81: 100994.     CrossRef
  • JEV-nanobarcode and colorimetric reverse transcription loop-mediated isothermal amplification (cRT-LAMP)
    Gna Ahn, Se Hee Lee, Min-Suk Song, Beom-Ku Han, Yang-Hoon Kim, Ji-Young Ahn
    Microchimica Acta.2021;[Epub]     CrossRef
  • Review of Emerging Japanese Encephalitis Virus: New Aspects and Concepts about Entry into the Brain and Inter-Cellular Spreading
    Luis Filgueira, Nils Lannes
    Pathogens.2019; 8(3): 111.     CrossRef
  • Seroprevalence of Dengue Virus Antibody in Korea
    Ji Hyen Lee, Han Wool Kim, Kyung-Hyo Kim
    Pediatric Infection & Vaccine.2018; 25(3): 132.     CrossRef
  • Japanese Encephalitis: A Brief Review on Indian Perspectives
    Reshma Kulkarni, Gajanan N. Sapkal, Himanshu Kaushal, Devendra T. Mourya
    The Open Virology Journal.2018; 12(1): 121.     CrossRef
  • Clinical Characteristics of Severe Japanese Encephalitis: A Case Series from South Korea
    Kyung-Il Park, Manho Kim, Jun-Sang Sunwoo, Ki-Young Jung, Kon Chu, Keun-Hwa Jung, Sang Kun Lee, Soon-Tae Lee, Jangsup Moon
    The American Journal of Tropical Medicine and Hygi.2017; 97(2): 369.     CrossRef
  • JAPANESE ENCEPHALITIS, RECENT PERSPECTIVES ON VIRUS GENOME, TRANSMISSION, EPIDEMIOLOGY, DIAGNOSIS AND PROPHYLACTIC INTERVENTIONS
    Arumugam Karthikeyan, Subramaniyan Shanmuganathan, Selvaraj Pavulraj, Govinthasamy Prabakar, Selvaraj Pavithra, Kannan Porteen, Govindaraj Elaiyaraja, Yashpal Singh Malik
    Journal of Experimental Biology and Agricultural S.2017; 5(6): 730.     CrossRef
  • Prevalence of Neutralizing Antibodies to Japanese Encephalitis Virus among High-Risk Age Groups in South Korea, 2010
    Eun Ju Lee, Go-Woon Cha, Young Ran Ju, Myung Guk Han, Won-Ja Lee, Young Eui Jeong, Nagendra R Hegde
    PLOS ONE.2016; 11(1): e0147841.     CrossRef
  • A Novel Immunochromatographic Test Applied to a Serological Survey of Japanese Encephalitis Virus on Pig Farms in Korea
    Go-Woon Cha, Eun Ju Lee, Eun-Joo Lim, Kang Suk Sin, Woo Won Park, Doo Young Jeon, Myung Guk Han, Won-Ja Lee, Woo-Young Choi, Young Eui Jeong, Lark L. Coffey
    PLOS ONE.2015; 10(5): e0127313.     CrossRef
  • Silent Circulation of Ross River Virus in French Polynesia
    Maite Aubry, Jérôme Finke, Anita Teissier, Claudine Roche, Julien Broult, Sylvie Paulous, Philippe Desprès, Van-Mai Cao-Lormeau, Didier Musso
    International Journal of Infectious Diseases.2015; 37: 19.     CrossRef
Development of a Diagnostic Kit to Detect Cryptosporidium parvum and Giardia lamblia
Hyeng-Il Cheun, Byung-Suk Chung, Da-Won Ma, Bo-La Goo, Shin-Hyeong Cho, Mi-jung Ji, Won-Ja Lee
Osong Public Health Res Perspect. 2013;4(3):146-151.   Published online June 30, 2013
DOI: https://doi.org/10.1016/j.phrp.2013.04.006
  • 4,229 View
  • 24 Download
  • 7 Crossref
AbstractAbstract PDF
Objectives
This study aims to develop a high-sensitivity antibody diagnostic kit that will enable a rapid and accurate detection of Cryptospofidium parvum and Giardia lamblia in patients with diarrhea.
Methods
The cultivated C. parvum oocysts and G. lamblia cysts in each calf and dog were injected to mice to obtain antibodies, which were titrated. Spleen cells of the immunized mouse were separated and blended with myelomas to produce hybrid cell lines that form monoclonal antibodies. Using ELISA method, antibodies that specifically respond to C. parvum and G.lamblia were then selected. The cells were injected into the abdominal cavity of a BALB/c mouse to isolate hydrops abdominis containing high level of antibodies. The IgG antibody was purified using protein G gel.
Results
The detection limit of monoclonal antibodies for Cryptosporidium parvum and Giardia lamblia was 125 oocysts/mL and 1250 cysts/mL, respectively. In addition, during testing they did not show cross-reactivity to viruses (n = 15), bacteria (n =17), and parasites (n = 9).
Conclusion
The rapid diagnostic antibody kit developed in this study, which specifically responds to C. parvum and G. lamblia, will be useful in detecting and monitoring diarrheal infections.

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  • Comprehensive review of conventional and state-of-the-art detection methods of Cryptosporidium
    George Luka, Ehsan Samiei, Nishat Tasnim, Arash Dalili, Homayoun Najjaran, Mina Hoorfar
    Journal of Hazardous Materials.2022; 421: 126714.     CrossRef
  • A review ofCryptosporidiumspp. and their detection in water
    Eman M. Hassan, Banu Örmeci, Maria C. DeRosa, Brent R. Dixon, Syed A. Sattar, Asma Iqbal
    Water Science and Technology.2021; 83(1): 1.     CrossRef
  • Portable on-chip colorimetric biosensing platform integrated with a smartphone for label/PCR-free detection of Cryptosporidium RNA
    George S. Luka, Ephraim Nowak, Quin Robert Toyata, Nishat Tasnim, Homayoun Najjaran, Mina Hoorfar
    Scientific Reports.2021;[Epub]     CrossRef
  • Nanomaterials‐based biosensors for detection of microorganisms and microbial toxins
    Laura Sutarlie, Sian Yang Ow, Xiaodi Su
    Biotechnology Journal.2017;[Epub]     CrossRef
  • ACG Clinical Guideline: Diagnosis, Treatment, and Prevention of Acute Diarrheal Infections in Adults
    Mark S Riddle, Herbert L DuPont, Bradley A Connor
    American Journal of Gastroenterology.2016; 111(5): 602.     CrossRef
  • Multiplex-Touchdown PCR to Simultaneously Detect Cryptosporidium parvum, Giardia lamblia, and Cyclospora cayetanensis, the Major Causes of Traveler’s Diarrhea
    Ji-Hun Shin, Sang-Eun Lee, Tong Soo Kim, Da-Won Ma, Jong-Yil Chai, Eun-Hee Shin
    The Korean Journal of Parasitology.2016; 54(5): 631.     CrossRef
  • Advancing Cryptosporidium Diagnostics from Bench to Bedside
    Raul V. Destura, Rohani B. Cena, Ma. Jowina H. Galarion, Coleen M. Pangilinan, Geraldine M. Arevalo, Ryan Oliver C. Alba, Joy Ann G. Petronio, Gielenny M. Salem, Brian Schwem, Jesus Emmanuel A. D. Sevilleja
    Current Tropical Medicine Reports.2015; 2(3): 150.     CrossRef
The First Outbreak of Giardiasis with Drinking Water in Korea
Hyeng-Il Cheun, Cheon-Hyeon Kim, Shin-Hyeong Cho, Da-Won Ma, Bo-La Goo, Mun-Su Na, Seung-Ki Youn, Won-Ja Lee
Osong Public Health Res Perspect. 2013;4(2):89-92.   Published online April 30, 2013
DOI: https://doi.org/10.1016/j.phrp.2013.03.003
  • 3,300 View
  • 24 Download
  • 25 Crossref
AbstractAbstract PDF
Objectives
To identify the pathogen of the diarrhea outbreak in a village in Jeollabuk province in Korea in April 2010.
Methods
DNA extraction was performed from the 120 L of collected water, which was centrifuged at 10,000 x g for 30 min. PCR reactions were conducted in a total of 25 ul, which included PCR premix (GenDEPOT, Barker, TX, USA), 2 ul (∼100 ng) of extracted DNA, and 10 pmol of each primer.
Results
Nine people out of 25 had a symptom of abdominal pain accompanied by diarrhea after they used stored valley water in a water tank as a provisional water supply source without chlorine sterilization. Among them Giardia lamblia was detected in fecal samples of 7 people using the polymerase chain reaction method. Although G. lamblia was also detected from water provided by the provisional water supply system stored in the water tank and used as drinking water, it was not detected in the water tank itself. This water-borne outbreak is considered to have occurred when the provisional water supply tube was destroyed under a building construction and contaminated by G. lamblia, but its precise cause has not been clarified.
Conclusion
This outbreak resulting from G. lamblia is very meaningful as the first outbreak of an infection by a water-borne parasite in Korea.

Citations

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  • Waterborne Cryptosporidium and Giardia in resources of MENA: a systematic review and meta-analysis
    Layla Ben Ayed, Shahira Abdelaziz Ali Ahmed, Sonia Boughattas, Panagiotis Karanis
    Journal of Water and Health.2024;[Epub]     CrossRef
  • Molecular detection and assemblage analysis of the intestinal protozoan Giardia duodenalis in wild boars in Korea
    Haeseung Lee, Dongmi Kwak
    Frontiers in Veterinary Science.2023;[Epub]     CrossRef
  • A scoping review of risk factors and transmission routes associated with human giardiasis outbreaks in high-income settings
    Sarah Krumrie, Paul Capewell, Alison Smith-Palmer, Dominic Mellor, Willie Weir, Claire L. Alexander
    Current Research in Parasitology & Vector-Borne Di.2022; 2: 100084.     CrossRef
  • Prevalence and molecular characterization of Giardia duodenalis in small ruminants of Shiraz, southwestern Iran: A zoonotic concern
    Ali Asghari, Farzad Mahdavi, Laya Shamsi, Mohammad Hossein Motazedian, Qasem Asgari, Saeed Shahabi, Behnam Mohammadi-Ghalehbin, Alireza Sadrebazzaz
    Comparative Immunology, Microbiology and Infectiou.2022; 86: 101819.     CrossRef
  • Genotypic and Epidemiologic Profiles of Giardia duodenalis in Four Brazilian Biogeographic Regions
    Deiviane A. Calegar, Beatriz C. Nunes, Kerla J. L. Monteiro, Polyanna A. A. Bacelar, Brenda B. C. Evangelista, Mayron M. Almeida, Jurecir Silva, Jéssica P. Santos, Márcio N. Boia, Lauren H. Jaeger, Filipe A. Carvalho-Costa
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    Water Environment Research.2022;[Epub]     CrossRef
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  • Development of a simple, rapid multiplex PCR tool kit by using the 16S rRNA gene for the identification of faecal and non-faecal coliforms in drinking water
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    Water Supply.2021; 21(7): 3319.     CrossRef
  • Use of the bivalve Dreissena polymorpha as a biomonitoring tool to reflect the protozoan load in freshwater bodies
    Elodie Géba, Dominique Aubert, Loïc Durand, Sandy Escotte, Stéphanie La Carbona, Catherine Cazeaux, Isabelle Bonnard, Fanny Bastien, Mélissa Palos Ladeiro, Jitender P. Dubey, Isabelle Villena, Alain Geffard, Aurélie Bigot-Clivot
    Water Research.2020; 170: 115297.     CrossRef
  • Multilocus genotyping of Giardia duodenalis from pigs in Korea
    Haeseung Lee, ByeongYeal Jung, Jun-Sik Lim, Min-Goo Seo, Seung-Hun Lee, Kwang-Ho Choi, Mi-Hye Hwang, Tae-Hwan Kim, Oh-Deog Kwon, Dongmi Kwak
    Parasitology International.2020; 78: 102154.     CrossRef
  • Multilocus genotyping and risk factor analysis of Giardia duodenalis in dogs in Korea
    Ha-Young Kim, Haeseung Lee, Seung-Hun Lee, Min-Goo Seo, Seonju Yi, Jong Wan Kim, Chung Hyun Kim, Yu-Ran Lee, ByungJae So, Oh-Deog Kwon, Dongmi Kwak
    Acta Tropica.2019; 199: 105113.     CrossRef
  • Molecular Prevalence and Genotypes of Cryptosporidium parvum and Giardia duodenalis in Patients with Acute Diarrhea in Korea, 2013-2016
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    The Korean Journal of Parasitology.2019; 57(5): 531.     CrossRef
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  • Identification of zoonotic Giardia duodenalis in Korean native calves with normal feces
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    Parasitology Research.2018; 117(6): 1969.     CrossRef
  • Waterborne transmission of protozoan parasites: Review of worldwide outbreaks - An update 2011–2016
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    Revista Brasileira de Parasitologia Veterinária.2015; 24(3): 303.     CrossRef
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Longitudinal and Cross-Sectional Genetic Diversity in the Korean Peninsula Based on the P vivax Merozoite Surface Protein Gene [Volume 2, Issue 3, December 2011, Pages 158 - 163]
Jung-Yeon Kim, Eun-Jung Suh, Hyo-Soon Yu, Hyun-Sik Jung, In-Ho Park, Yien-Kyeoug Choi, Kyoung-Mi Choi, Shin-Hyeong Cho, Won-Ja Lee
Osong Public Health Res Perspect. 2012;3(1):63-63.   Published online December 31, 2011
DOI: https://doi.org/10.1016/j.phrp.2011.11.039.
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Longitudinal and Cross-Sectional Genetic Diversity in the Korean Peninsula Based on the P vivax Merozoite Surface Protein Gene
Jung-Yeon Kim, Eun-Jung Suh, Hyo-Soon Yu, Hyun-Sik Jung, In-Ho Park, Yien-Kyeoug Choi, Kyoung-Mi Choi, Shin-Hyeong Cho, Won-Ja Lee
Osong Public Health Res Perspect. 2011;2(3):158-163.   Published online December 31, 2011
DOI: https://doi.org/10.1016/j.phrp.2011.11.039
  • 3,210 View
  • 18 Download
  • 4 Crossref
AbstractAbstract PDF
Objectives
Vivax malaria has reemerged and become endemic in Korea. Our study aimed to analyze by both longitudinal and cross-sectional genetic diversity of this malaria based on the P vivax Merozoite Surface Protein (PvMSP) gene parasites recently found in the Korean peninsula.
Methods
PvMSP-1 gene sequence analysis from P vivax isolates (n = 835) during the 1996-2010 period were longitudinally analyzed and the isolates from the Korean peninsula through South Korea, the demilitarized zone and North Korea collected in 2008-2010 were enrolled in an overall analysis of MSP-1 gene diversity.
Results
New recombinant subtypes and severe multiple-cloneinfection rates were observed in recent vivax parasites. Regional variation was also observed in the study sites.
Conclusion
This study revealed the great complexity of genetic variation and rapid dissemination of genes in P vivax. It also showed interesting patterns of diversity depending, on the region in the Korean Peninsula. Understanding the parasiteninsula. Under genetic variation may help to analyze trends and assess the extent of endemic malaria in Korea.

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  • Molecular surveillance over 14 years confirms reduction of Plasmodium vivax and falciparum transmission after implementation of Artemisinin-based combination therapy in Papua, Indonesia
    Zuleima Pava, Agatha M. Puspitasari, Angela Rumaseb, Irene Handayuni, Leily Trianty, Retno A. S. Utami, Yusrifar K. Tirta, Faustina Burdam, Enny Kenangalem, Grennady Wirjanata, Steven Kho, Hidayat Trimarsanto, Nicholas M. Anstey, Jeanne Rini Poespoprodjo,
    PLOS Neglected Tropical Diseases.2020; 14(5): e0008295.     CrossRef
  • Distribution of Antibodies Specific to the 19-kDa and 33-kDa Fragments of Plasmodium vivax Merozoite Surface Protein 1 in Two Pathogenic Strains Infecting Korean Vivax Malaria Patients
    Sylvatrie-Danne Dinzouna-Boutamba, Sanghyun Lee, Ui-Han Son, Su-Min Song, Hye Soo Yun, So-Young Joo, Dongmi Kwak, Man Hee Rhee, Dong-Il Chung, Yeonchul Hong, Youn-Kyoung Goo
    Osong Public Health and Research Perspectives.2016; 7(4): 213.     CrossRef
  • The unique distribution of the Plasmodium vivax merozoite surface protein 1 in parasite isolates with short and long latent periods from the Republic of Korea
    Youn-Kyoung Goo, Jun-Hye Moon, So-Young Ji, Dong-Il Chung, Yeonchul Hong, Shin-Hyung Cho, Won-Ja Lee, Jung-Yeon Kim
    Malaria Journal.2015;[Epub]     CrossRef
  • Evaluation of single nucleotide polymorphisms of pvmdr1 and microsatellite genotype in Plasmodium vivax isolates from Republic of Korea military personnel
    Dong-Il Chung, Sookwan Jeong, Sylvatrie-Danne Dinzouna-Boutamba, Hye-Won Yang, Sang-Geon Yeo, Yeonchul Hong, Youn-Kyoung Goo
    Malaria Journal.2015;[Epub]     CrossRef
Prevalence of Clonorchis sinensis Infections Along the Five Major Rivers in Republic of Korea, 2007
Hyun-Kyung Kim, Hyeng-Il Cheun, Byung-Suk Cheun, Ki-Yeon Lee, Tong-Soo Kim, Sang-Eun Lee, Won-ja Lee, Shin-Hyeong Cho
Osong Public Health Res Perspect. 2010;1(1):43-49.   Published online December 31, 2010
DOI: https://doi.org/10.1016/j.phrp.2010.12.010
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  • 21 Crossref
AbstractAbstract PDF
Objectives
The prevalence of Clonorchis sinensis infection was investigated among residents of the five major river basins, that is, Hangang, Nakdonggang, Seomjingang, Yeongsangang, and Geumgang River basins in Korea.
Methods
From January to December 2007, a total of 31,268 stool samples were collected from 29 localities and examined by the formalin-ether sedimentation technique.
Results
Intestinal parasite eggs and/or protozoan cysts were detected from 2957 (9.5%) inhabitants. Number of residents harbouring helminth eggs in the faeces was 2542 (8.1%) for C. sinensis, 255 (0.8%) for Heterophyes spp., 36 (0.1%) for Echinostoma spp., 30 (0.1%) for Trichuris trichiura, 8 (0.03%) for Ascaris lumbricoides, 7 (0.02%) for Gymnophalloide seoi, and 50 (0.02%) for Trichostrongylus orientalis. Number of residents harbouring protozoan cysts in the faeces was 133 (1.3%) for Entamoeba spp. and 50 (0.2%) for Giardia lamblia. The positive rates of C. sinensis in Nakdonggang, Seomjingang, Yeongsangang, Geumgang, and Hangang River basins were 12.2%, 9.5%, 3.3%, 3.0%, and 1.0%, respectively. The egg positive rate of C. sinensis was higher in male (10.6%) than in female (6.1%), and the age group of 50s had the highest positive rate (10.4%).
Conclusion
The result of this study revealed little decrease in positive rate of C. sinensis compared with the result of southern endemic areas of Korea in 2006.

Citations

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  • Global prevalence of 4 neglected foodborne trematodes targeted for control by WHO: A scoping review to highlight the gaps
    Rachel Tidman, Kaushi S. T. Kanankege, Mathieu Bangert, Bernadette Abela-Ridder, Paul R. Torgerson
    PLOS Neglected Tropical Diseases.2023; 17(3): e0011073.     CrossRef
  • Infection Characteristics of Clonorchis sinensis Metacercariae in Fish from Republic of Korea
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    The Korean Journal of Parasitology.2022; 60(2): 79.     CrossRef
  • High Endemicity with Clonorchis sinensis Metacercariae in Fish from Yongjeon-cheon (Stream) in Cheongsong-gun, Gyeongsangbuk-do, Korea
    Woon-Mok Sohn, Byoung-Kuk Na, Shin-Hyeong Cho, Hee Il Lee, Myoung-Ro Lee, Jung-Won Ju, Gou Ok Kim
    The Korean Journal of Parasitology.2021; 59(1): 97.     CrossRef
  • Survey of Zoonotic Trematode Metacercariae in Fish from Water Systems of Geum-gang (River) in Republic of Korea
    Woon-Mok Sohn, Byoung-Kuk Na, Shin-Hyeong Cho, Cheon-Hyeon Kim, Min-Ah Hwang, Kyeong-Woo No, Jai-Dong Kim
    The Korean Journal of Parasitology.2021; 59(1): 23.     CrossRef
  • Risk Factors for Clonorchis sinensis Infection in Residents of Binyang, Guangxi: A Cross-Sectional and Logistic Analysis Study
    Meng Xu, Yanyan Jiang, Jianhai Yin, Shengkui Cao, Yujuan Shen, Jianping Cao
    Frontiers in Public Health.2021;[Epub]     CrossRef
  • Prevalence and Infection Intensity of Zoonotic Trematode Metacercariae in Fish from Soyang-cheon (Stream), in Wanju-gun, Jeollabuk-do, Korea
    Woon-Mok Sohn, Byoung-Kuk Na, Shin-Hyeong Cho, Jung-Won Ju, Cheon-Hyeon Kim, Min-Ah Hwang, Kyeong-Woo No, Jong-Ho Park
    The Korean Journal of Parasitology.2021; 59(3): 265.     CrossRef
  • Survey of Zoonotic Trematode Metacercariae in Fish from Irrigation Canal of Togyo-jeosuji (Reservoir) in Cheorwon-gun, Gangwon-do, Republic of Korea
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    The Korean Journal of Parasitology.2021; 59(4): 427.     CrossRef
  • Endemicity of Zoonotic Trematode Metacercariae in Fish from Deokcheon-gang (River) in Sancheong-gun, Gyeongsangnam-do, Republic of Korea
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    The Korean Journal of Parasitology.2021; 59(5): 523.     CrossRef
  • Prevalence of Clonorchis sinensis infection in fish in South‐East Asia: A systematic review and meta‐analysis
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    Journal of Fish Diseases.2020; 43(11): 1409.     CrossRef
  • Infection Status with Clonorchis sinensis Metacercariae in Fish from Yangcheon (Stream) in Sancheong-gun, Gyeongsangnam-do, Korea
    Woon-Mok Sohn, Byoung-Kuk Na, Shin-Hyeong Cho, Jung-Won Ju
    The Korean Journal of Parasitology.2019; 57(2): 145.     CrossRef
  • Prevalence, Species Distribution, and Related Factors of Fish-Borne Trematode Infection in Ninh Binh Province, Vietnam
    Anh Kieu Thi Tran, Hoa Thuy Doan, Anh Ngoc Do, Van Thi Nguyen, Su Xuan Hoang, Huong Thu Thi Le, Hoa Thi Hoang, Nam Hoang Le, Quyen Bao Thi Le, Tran-Anh Le
    BioMed Research International.2019; 2019: 1.     CrossRef
  • Infection Status with Digenetic Trematode Metacercariae in Fishes from Coastal Lakes in Gangwon-do, Republic of Korea
    Woon-Mok Sohn, Byoung-Kuk Na, Shin-Hyeong Cho, Soon-Won Lee
    The Korean Journal of Parasitology.2019; 57(6): 681.     CrossRef
  • Recombinant adenylate kinase 3 from liver fluke Clonorchis sinensis for histochemical analysis and serodiagnosis of clonorchiasis
    Soon Bin Kwon, Paul Kim, Hae Sun Woo, Tae Yun Kim, Ju Yeong Kim, Hye Min Lee, Yun Soo Jang, Eun-Min Kim, Tai-Soon Yong, Baik Lin Seong
    Parasitology.2018; 145(12): 1531.     CrossRef
  • Prevalence and Intensity of Clonorchis sinensis Metacercariae in Freshwater Fish from Wicheon Stream in Gunwi-gun, Gyeongsangbuk-do, Korea
    Woon-Mok Sohn, Byoung-Kuk Na, Shin-Hyeong Cho, Jung-Won Ju, Dong-Chul Son
    The Korean Journal of Parasitology.2018; 56(1): 41.     CrossRef
  • Epidemiological and Clinical Parameters Features of Patients with Clonorchiasis in the Geum River Basin, Republic of Korea
    Hee-Eun Shin, Myoung-Ro Lee, Jung-Won Ju, Byong-Suk Jeong, Mi-Yeoun Park, Keoung-Sook Lee, Shin-Hyeong Cho
    Interdisciplinary Perspectives on Infectious Disea.2017; 2017: 1.     CrossRef
  • A new method for estimating the prevalence of clonorchiasis in Korea
    Jin-Young Jeong, Jin Yong Lee, Byung-Suk Chung, Younghyun Choi, Allison Baer Alley, Hyun Joo Kim
    Medicine.2017; 96(13): e6536.     CrossRef
  • Prevalence of Clonorchis sinensis Infection among Residents along 5 Major Rivers in the Republic of Korea
    Young-Il Jeong, Hee-Eun Shin, Sang-Eun Lee, Hyeng-Il Cheun, Jung-Won Ju, Jung-Yeon Kim, Mi Yeoun Park, Shin-Hyeong Cho
    The Korean Journal of Parasitology.2016; 54(2): 215.     CrossRef
  • Trematode Metacercariae in Freshwater Fish from Water Systems of Hantangang and Imjingang in Republic of Korea
    Woon-Mok Sohn, Byoung-Kuk Na, Shin-Hyeong Cho, Soon-Won Lee, Seung-Bong Choi, Won-Seok Seok
    The Korean Journal of Parasitology.2015; 53(3): 289.     CrossRef
  • Prevalence and Risk Factors of Clonorchiasis among Residents of Riverside Areas in Muju-gun, Jeollabuk-do, Korea
    Do-Soon Park, Sung-Jin Na, Shin Hyeong Cho, Kyung Ja June, Young-Chae Cho, Young-Ha Lee
    The Korean Journal of Parasitology.2014; 52(4): 391.     CrossRef
  • Prevalence and Risk Factors of Clonorchiasis among the Populations Served by Primary Healthcare Posts along Five Major Rivers in South Korea
    Kyung Ja June, Shin Hyeong Cho, Won Ja Lee, Chunmi Kim, Kyung-Soon Park
    Osong Public Health and Research Perspectives.2013; 4(1): 21.     CrossRef

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