Skip Navigation
Skip to contents

PHRP : Osong Public Health and Research Perspectives



Page Path
HOME > Search
1 "Alireza H. Tasbiti"
Article category
Publication year
Original Article
Comparison of Three Different Methods for Detection of IL28 rs12979860 Polymorphisms as a Predictor of Treatment Outcome in Patients with Hepatitis C Virus
Abolfazl Fateh, Mohammadreza Aghasadeghi, Seyed D. Siadat, Farzam Vaziri, Farzin Sadeghi, Roohollah Fateh, Hossein Keyvani, Alireza H. Tasbiti, Shamsi Yari, Angila Ataei-Pirkooh, Seyed H. Monavari
Osong Public Health Res Perspect. 2016;7(2):83-89.   Published online April 30, 2016
  • 2,692 View
  • 18 Download
  • 21 Citations
AbstractAbstract PDF
This study aimed to evaluate the specificity, sensitivity, cost, and turn-around time of three methods of gene polymorphism analysis and to study the relationship between IL28B rs12979860 and SVR rate to pegIFN-α/RVB therapy among patients with chronic hepatitis C.
A total of 100 samples from chronic hepatitis C patients were analyzed in parallel using the three methods: direct sequencing, real-time polymerase chain reaction (PCR), amplification refractory mutation system (ARMS)-PCR.
The different profiles for IL28B rs12979860 alleles (CC, CT, and TT) obtained with PCR-RFLP, ARMS-PCR, and direct sequencing were consistent among the three methods. Prevalence of rs12979860 genotypes CC, CT and TT in HCV genotype 1a was 10(19.6%), 35(68.6%), and six (11.8%), respectively, and in HCV genotype 31, it was 13(26.5%), 31(63.3%), and five (10.2%), respectively. No significant difference was seen between rs12979860 genotype and HCV genotype (p = 0.710).
Screening by ARMS – PCR SNOP detection represents the most efficient and reliable method to determine HCV polymorphisms in routine clinical practice.


Citations to this article as recorded by  
  • Multiplex Snapshot Minisequencing for the Detection of Common PAH Gene Mutations in Iranian Patients with Phenylketonuria
    Pegah Namdar Aligoodarzi, Golale Rostami, Seyed Reza Kazemi Nezhad, Mohammad Hamid
    Iranian Biomedical Journal.2023; 27(1): 46.     CrossRef
  • Expression of TRIM56 gene in SARS-CoV-2 variants and its relationship with progression of COVID-19
    Rezvan Tavakoli, Pooneh Rahimi, Mojtaba Hamidi-Fard, Sana Eybpoosh, Delaram Doroud, Iraj Ahmadi, Enayat Anvari, Mohammadreza Aghasadeghi, Abolfazl Fateh
    Future Virology.2023; 18(9): 563.     CrossRef
  • Performance of the tetra-primer PCR technique compared to PCR-RFLP in the search for rs12979860 (C/T) and rs8099917 (T/G) single nucleotide polymorphisms (SNPs) in the IFNL4 gene
    Ellen Hochleitner Souza Kindermann, Karoline Rodrigues Campos, Adele Caterino-de-Araujo
    Revista do Instituto Adolfo Lutz.2023; 82: 1.     CrossRef
  • Glioblastoma as a Novel Drug Repositioning Target: Updated State
    Hamed Hosseinalizadeh, Ammar Ebrahimi, Ahmad Tavakoli, Seyed Hamidreza Monavari
    Anti-Cancer Agents in Medicinal Chemistry.2023; 23(11): 1253.     CrossRef
  • MicroRNAs Profiling in HIV, HCV, and HIV/HCV Co-Infected Patients
    Mohsen Moghoofei, Sohrab Najafipour, Shayan Mostafaei , Ahmad Tavakoli , Farah Bokharaei-Salim , Saied Ghorbani, Davod Javanmard, Hadi Ghaffari , Seyed Hamidreza Monavari
    Current HIV Research.2021; 19(1): 27.     CrossRef
  • Occult hepatitis C virus infection in hemophilia patients and its correlation with interferon lambda 3 and 4 polymorphisms
    Amir Hossein Nafari, Ahmad Ayadi, Zahra Noormohamadi, Fatemeh Sakhaee, Farzam Vaziri, Seyed Davar Siadat, Abolfazl Fateh
    Infection, Genetics and Evolution.2020; 79: 104144.     CrossRef
  • Polymerase Chain Reaction Assay Using the Restriction Fragment Length Polymorphism Technique in the Detection of Prosthetic Joint Infections: A Multi-Centered Study
    Ataollah Moshirabadi, Mohammad Razi, Peyman Arasteh, Mohammad Mahdi Sarzaeem, Saman Ghaffari, Saied Aminiafshar, Kami Hosseinian Khosroshahy, Fatemeh Maryam Sheikholeslami
    The Journal of Arthroplasty.2019; 34(2): 359.     CrossRef
  • One-Step ARMS-PCR for the Detection of SNPs—Using the Example of the PADI4 Gene
    Ehnert, Linnemann, Braun, Botsch, Leibiger, Hemmann, Nussler
    Methods and Protocols.2019; 2(3): 63.     CrossRef
  • Epstein–Barr virus and risk of breast cancer: a systematic review and meta-analysis
    Mohammad Farahmand, Seyed Hamidreza Monavari, Zabihollah Shoja, Hadi Ghaffari, Mehdi Tavakoli, Ahmad Tavakoli
    Future Oncology.2019; 15(24): 2873.     CrossRef
  • Modeling suggests that microliter volumes of contaminated blood caused an outbreak of hepatitis C during computerized tomography
    Eyal Shteyer, Louis Shekhtman, Tal Zinger, Sheri Harari, Inna Gafanovich, Dana Wolf, Hefziba Ivgi, Rima Barsuk, Ilana Dery, Daniela Armoni, Mila Rivkin, Rahul Pipalia, Michal Cohen Eliav, Yizhak Skorochod, Gabriel S. Breuer, Ran Tur-kaspa, Yonit Weil Wien
    PLOS ONE.2019; 14(1): e0210173.     CrossRef
  • Correlation of CD81 and SCARB1 polymorphisms on virological responses in Iranian patients with chronic hepatitis C virus genotype 1
    Milad Nafari, Shiva Irani, Farzam Vaziri, Safoora Gharibzadeh, Fatemeh Sakhaee, Mohammad Khazeni, Naser Kalhor, Fatemeh Rahimi Jamnani, Seyed Davar Siadat, Abolfazl Fateh
    Infection, Genetics and Evolution.2018; 62: 296.     CrossRef
  • First detection of human hepegivirus-1 (HHpgV-1) in Iranian patients with hemophilia
    Yazdan Bijvand, Mohammad Reza Aghasadeghi, Fatemeh Sakhaee, Parviz Pakzad, Farzam Vaziri, Alireza Azizi Saraji, Fatemeh Rahimi Jamnani, Seyed Davar Siadat, Abolfazl Fateh
    Scientific Reports.2018;[Epub]     CrossRef
  • Evaluation of TRIM5 and TRIM22 polymorphisms on treatment responses in Iranian patients with chronic hepatitis C virus infection
    Setareh Mobasheri, Nazanin Irani, Abbas Akhavan Sepahi, Fatemeh Sakhaee, Fatemeh Rahimi Jamnani, Farzam Vaziri, Seyed Davar Siadat, Abolfazl Fateh
    Gene.2018; 676: 95.     CrossRef
  • Data Mining and Machine Learning Algorithms Using IL28B Genotype and Biochemical Markers Best Predicted Advanced Liver Fibrosis in Chronic Hepatitis C
    Hend Ibrahim Shousha, Abubakr Hussein Awad, Dalia Abdelhamid Omran, Mayada Mohamed Elnegouly, Mahasen Mabrouk
    Japanese Journal of Infectious Diseases.2018; 71(1): 51.     CrossRef
  • IL28B rs12980275 and HLA rs4273729 genotypes as a powerful predictor factor for rapid, early, and sustained virologic response in patients with chronic hepatitis C
    Parvaneh Sedighimehr, Shiva Irani, Fatemeh Sakhaee, Farzam Vaziri, Mohammadreza Aghasadeghi, Seyed Mehdi Sadat, Fatemeh Rahimi Jamnani, Abolfazl Fateh, Seyed Davar Siadat
    Archives of Virology.2017; 162(1): 181.     CrossRef
  • Comparison of Direct Sequencing, Real-Time PCR-High Resolution Melt (PCR-HRM) and PCR-Restriction Fragment Length Polymorphism (PCR-RFLP) Analysis for Genotyping of Common Thiopurine Intolerant Variant Alleles NUDT15 c.415C>T and TPMT c.719A>G (TPMT*3C)
    Wai-Ying Fong, Chi-Chun Ho, Wing-Tat Poon
    Diagnostics.2017; 7(2): 27.     CrossRef
  • Effect of IL15 rs10833 and SCARB1 rs10846744 on virologic responses in chronic hepatitis C patients treated with pegylated interferon-α and ribavirin
    Sahar Sadeghi, Mehdi Davari, Esmaeil Asli, Safoora Gharibzadeh, Farzam Vaziri, Fatemeh Rahimi Jamnani, Abolfazl Fateh, Seyed Davar Siadat
    Gene.2017; 630: 28.     CrossRef
  • High platelet count and high probability of CALR detection in myeloproliferative neoplasms
    Reza Shirzad, Zari Tahan-nejad, Javad Mohamadi-asl, Mohammad Seghatoleslami, Ahmad Ahmadzadeh, Amal Saki Malehi, Najmaldin Saki
    Comparative Clinical Pathology.2017; 26(1): 25.     CrossRef
  • A comparative study of various methods for detection of IL28B rs12979860 in chronic hepatitis C
    Seyed Hamidreza Monavari, Roohollah Fateh, Farzam Vaziri, Fatemeh Rahimi Jamnani, Enayat Anvari, Farzin Sadeghi, Parviz Afrough, Ava Behrouzi, Fatemeh Sakhaee, Sepideh Meidaninikjeh, Hamidreza Mollaie, Alireza Hadizadeh Tasbiti, Shamsi Yari, Maryam Sadegh
    Scandinavian Journal of Clinical and Laboratory In.2017; 77(4): 247.     CrossRef
  • EGFR rs11506105 and IFNL3 SNPs but not rs8099917 are strongly associated with treatment responses in Iranian patients with chronic hepatitis C
    M Asnavandi, M Zargar, F Vaziri, F R Jamnani, S Gharibzadeh, A Fateh, S D Siadat
    Genes & Immunity.2017; 18(3): 144.     CrossRef
  • Genetic Variation in Interleukin-28B and Response to Peg-IFNα-2a/RBV Combination Therapy in Patients with Hepatitis C Virus Infection
    Farah Bokharaei-Salim, Mostafa Salehi-Vaziri, Farzin Sadeghi, Khadijeh Khanaliha, Maryam Esghaei, Seyed Hamidreza Monavari, Seyed Moayed Alavian, Shahin Fakhim, Hossein Keyvani
    Jundishapur Journal of Microbiology.2016;[Epub]     CrossRef

PHRP : Osong Public Health and Research Perspectives