Skip Navigation
Skip to contents

PHRP : Osong Public Health and Research Perspectives



Page Path
HOME > Search
2 "Hae-Sun Chung"
Article category
Publication year
Corrigendum to “Investigation of Biofilm Formation and its Association with the Molecular and Clinical Characteristics of Methicillin-resistant Staphylococcus aureus” [Volume 4, Issue 5, October 2013, Pages 225–232]
Jeong-Ok Cha, Jae Il Yoo, Jung Sik Yoo, Hae-Sun Chung, Sun-Hee Park, Hwa Su Kim, Yeong Seon Lee, Gyung Tae Chung
Osong Public Health Res Perspect. 2014;5(2):116-116.   Published online April 30, 2014
  • 2,150 View
  • 19 Download
Original Article
Investigation of Biofilm Formation and its Association with the Molecular and Clinical Characteristics of Methicillin-resistant Staphylococcus aureus
Jeong-Ok Cha, Jae Il Yoo, Jung Sik Yoo, Hae-Sun Chung, Sun-Hee Park, Hwa Su Kim, Yeong Seon Lee, Gyung Tae Chung
Osong Public Health Res Perspect. 2013;4(5):225-232.   Published online October 31, 2013
  • 2,772 View
  • 13 Download
  • 50 Citations
AbstractAbstract PDF
To investigate the biofilm-forming related factors against MRSA bloodstream isolates and evaluates their clinical features and treatment outcomes by biofilm production.
We collected 126 consecutive methicillin-resistant Staphylococcus aureus (MRSA) causing blood stream infections (BSIs) at 10 tertiary hospitals from 2007 to 2009. We investigated biofilm-forming ability using a microtiter plate assay, and molecular characteristics including multilocus sequence typing, staphylococcal cassette chromosome mec and accessory gene regulator types. We compared the clinical characteristics and outcomes of patients infected with biofilm-forming and non-biofilm-forming MRSA isolates.
Of the 126 samples, 86 (68.3%), including 5 strong level (OD570 ≥ 1.0) and 81 weak level (0.2 ≤ OD570 < 1.0), had biofilm-forming capacity. Detection of fibronectinbinding protein in biofilm-forming strains was significantly higher than biofilm non-forming ones (p = 0.001) and three enterotoxin genes (sec-seg-sei) islands had a high frequency regardless of biofilm production. However, biofilm-forming strains were more likely to be multidrug resistant (three or more non-β-lactam antibiotics) than biofilm non-forming ones [79.2% vs. 59.2%, p = 0.015, odds ratio (OR) 2.629, 95% confidence interval (CI) 1.92–5.81]. Clinical features of patients with BSIs caused by biofilm-forming MRSA strains were more likely to be hospital onset [77.9% vs. 60.0%, p = 0.024, OR 2.434, 95% CI 1.11–5.33) and more frequently occurred in patients with use of invasive devices [85.7% vs. 61.2%, p = 0.002, OR 3.879, 95% CI 1.61–8.97]. The other clinical features were compared with the clinical outcomes of the two groups and were not significant (p > 0.05).
Biofilm-forming MRSA strains showed higher frequency of fnbB gene than biofilm non-forming ones and more incidence rates on particular genotypes. And, their patient's features were not significantly different between two groups in this study, except for several clinical factors.


Citations to this article as recorded by  
  • Liposomes-Based Drug Delivery Systems of Anti-Biofilm Agents to Combat Bacterial Biofilm Formation
    Zinb Makhlouf, Amaal Abdulraqeb Ali, Mohammad Hussein Al-Sayah
    Antibiotics.2023; 12(5): 875.     CrossRef
  • Staphylococcus aureus İzolatlarının Biyofilm Oluşturma Özelliklerinin Karşılaştırılması
    Demet GÜR VURAL, İlknur BIYIK, Elif Gülsüm TORUN, Yeliz TANRIVERDİ ÇAYCI, Kemal BİLGİN, Asuman BIRINCI
    Sağlık Bilimlerinde Değer.2023; 13(2): 245.     CrossRef
  • Tannin extracted from Penthorum chinense Pursh, a potential drug with antimicrobial and antibiofilm effects against methicillin-sensitive Staphylococcus aureus and methicillin-resistant Staphylococcus aureus
    Junyuan Qin, Lei Yu, Fu Peng, Xin Ye, Gangmin Li, Chen Sun, Fang Cheng, Cheng Peng, Xiaofang Xie
    Frontiers in Microbiology.2023;[Epub]     CrossRef
  • Antibacterial Activity of Boron Compounds Against Biofilm-Forming Pathogens
    Ozgur Celebi, Demet Celebi, Sumeyye Baser, Elif Aydın, Erva Rakıcı, Serpil Uğraş, Pınar Ağyar Yoldaş, Nurcan Kılıç Baygutalp, A. M. Abd El-Aty
    Biological Trace Element Research.2023;[Epub]     CrossRef
  • Biofilm Formation of Staphylococcus aureus from Pets, Livestock, and Wild Animals: Relationship with Clonal Lineages and Antimicrobial Resistance
    Vanessa Silva, Elisete Correia, José Eduardo Pereira, Camino González-Machado, Rosa Capita, Carlos Alonso-Calleja, Gilberto Igrejas, Patrícia Poeta
    Antibiotics.2022; 11(6): 772.     CrossRef
  • Sequence Type 5 (ST5) as a Possible Predictor of Bacterial Persistence in Adult Patients with Methicillin-Resistant Staphylococcus aureus Pneumonia Treated with Vancomycin
    Ya-Xin Fan, Meng-Ting Chen, Nan-Yang Li, Xiao-Fen Liu, Min-Jie Yang, Yuan-Cheng Chen, Xiao-Yu Liang, Ju-Fang Wu, Bei-Ning Guo, Si-Chao Song, Yong-Qiang Zhu, Feng-Ying Zhang, Jing-Qing Hang, Sheng-Bin Wu, Bo Shen, Hua-Yin Li, Qin Wang, Xu-Ming Luo, Qing-Ge
    Microbiology Spectrum.2022;[Epub]     CrossRef
  • Ten-step asymmetric total syntheses of potent antibiotics anthracimycin and anthracimycin B
    Peilin Tian, Wenkang Ye, Xiayan Zhang, Yi Tong, Pei-Yuan Qian, Rongbiao Tong
    Chemical Science.2022; 13(43): 12776.     CrossRef
  • Biofilm Formation and Prevalence of Biofilm-Related Genes Among Clinical Strains of Multidrug-Resistant Staphylococcus aureus
    Natalia Kaźmierczak, Bartłomiej Grygorcewicz, Lidia Piechowicz
    Microbial Drug Resistance.2021; 27(7): 956.     CrossRef
  • Correlation Between Biofilm-Formation and the Antibiotic Resistant Phenotype in Staphylococcus aureus Isolates: A Laboratory-Based Study in Hungary and a Review of the Literature
    Seyyed Askhan Senobar Tahaei, Anette Stájer, Ibrahim Barrak, Eszter Ostorházi, Dóra Szabó, Márió Gajdács
    Infection and Drug Resistance.2021; Volume 14: 1155.     CrossRef
  • Investigation of biofilm formation in methicillin-resistant Staphylococcus aureus associated with bacteraemia in a tertiary hospital
    Wen Kiong Niek, Cindy Shuan Ju Teh, Nuryana Idris, Kwai Lin Thong, Soo Tein Ngoi, Sasheela Sri La Sri Ponnampalavanar
    Folia Microbiologica.2021; 66(5): 741.     CrossRef
  • Biofilm Formation of Multidrug-Resistant MRSA Strains Isolated from Different Types of Human Infections
    Vanessa Silva, Luciana Almeida, Vânia Gaio, Nuno Cerca, Vera Manageiro, Manuela Caniça, José L. Capelo, Gilberto Igrejas, Patrícia Poeta
    Pathogens.2021; 10(8): 970.     CrossRef
  • Biofilm properties in relation to treatment outcome in patients with first-time periprosthetic hip or knee joint infection
    Karin Svensson Malchau, Jonatan Tillander, Magdalena Zaborowska, Maria Hoffman, Iñigo Lasa, Peter Thomsen, Henrik Malchau, Ola Rolfson, Margarita Trobos
    Journal of Orthopaedic Translation.2021; 30: 31.     CrossRef
  • Evaluation of antimicrobial peptide LL-37 for treatment of Staphylococcus aureus biofilm on titanium plate
    Jiantong Wei, Xuepeng Cao, Jun Qian, Zhixia Liu, Xulong Wang, Qinliuye Su, Yongpin Wang, Ruimin Xie, Xiang Li
    Medicine.2021; 100(44): e27426.     CrossRef
  • Biofilm Formation and its Association with Antibiotic Susceptibility Pattern in Methicillin-resistant Staphylococcus aureus isolates
    Bajarangi Lal Chaudhary, Dakshina Bisht, Sameer Singh Faujdar
    Journal of Pure and Applied Microbiology.2021; 15(4): 2041.     CrossRef
  • The Role of Staphylococcus aureus YycFG in Gene Regulation, Biofilm Organization and Drug Resistance
    Shizhou Wu, Junqi Zhang, Qi Peng, Yunjie Liu, Lei Lei, Hui Zhang
    Antibiotics.2021; 10(12): 1555.     CrossRef
  • Development of the Bacterial Spectrum and Antimicrobial Resistance in Surgical Site Infections of Trauma Patients
    Rico Eisner, Norman Lippmann, Christoph Josten, Arne C. Rodloff, Daniel Behrendt
    Surgical Infections.2020; 21(8): 684.     CrossRef
  • Reduction of Pseudomonas aeruginosa biofilm formation through the application of nanoscale vibration
    Shaun N. Robertson, Peter G. Childs, Ayorinde Akinbobola, Fiona L. Henriquez, Gordon Ramage, Stuart Reid, William G. Mackay, Craig Williams
    Journal of Bioscience and Bioengineering.2020; 129(3): 379.     CrossRef
  • Virulence factors and antimicrobial resistance of Staphylococcus aureus isolated from the production process of Minas artisanal cheese from the region of Campo das Vertentes, Brazil
    R.D. Castro, S.H.S.P. Pedroso, S.H.C. Sandes, G.O. Silva, K.C.M. Luiz, R.S. Dias, R.A.T. Filho, H.C.P. Figueiredo, S.G. Santos, A.C. Nunes, M.R. Souza
    Journal of Dairy Science.2020; 103(3): 2098.     CrossRef
  • Investigation of biofilm production and its association with genetic and phenotypic characteristics of OM (osteomyelitis) and non-OM orthopedic Staphylococcus aureus
    Shengpeng Yu, Bei Jiang, Chao Jia, Hongri Wu, Jie Shen, Xiaomei Hu, Zhao Xie
    Annals of Clinical Microbiology and Antimicrobials.2020;[Epub]     CrossRef
  • Busting biofilms: free-living amoebae disrupt preformed methicillin-resistant Staphylococcus aureus (MRSA) and Mycobacterium bovis biofilms
    Kevin H. Martin, Grace I. Borlee, William H. Wheat, Mary Jackson, Bradley R. Borlee
    Microbiology .2020; 166(8): 695.     CrossRef
  • Biofilm formation and molecular characterization of methicillin-resistant Staphylococcus aureus strains isolated from the patients, personnel, air and environment of ICUs
    Fatemeh Tahmasbi, Raheleh Sheikhi, Ali Ashraf, Ali Mojtahedi
    Gene Reports.2020; 20: 100736.     CrossRef
  • Characteristics of Clinically Significant Invasive Staphylococcus aureus Infections in a Tertiary Care Centre
    Gillaine Vail Pinto, Archana Bhat K., Sevitha Bhat
    Journal of Pure and Applied Microbiology.2020; 14(2): 1487.     CrossRef
  • Status of Biofilm-Forming Genes among Jordanian Nasal Carriers of Methicillin-Sensitive and Methicillin-Resistant Staphylococcus aureus
    Ashraf I Khasawneh, Nisreen Himsawi, Jumana Abu-Raideh, Muna A. Salameh, Mohammad Al-Tamimi, Sameer Al Haj Mahmoud, Tareq Saleh
    Iranian Biomedical Journal.2020; 24(6): 381.     CrossRef
  • Detection of Heavy Metal Tolerance among different MLSB Resistance Phenotypes of Methicillin-Resistant S. aureus (MRSA)
    Sara H. Mohamed, Maram M.S. Elshahed, Yasmine M. Saied, Mahmoud S.M. Mohamed, Gamal H. Osman
    Journal of Pure and Applied Microbiology.2020; 14(3): 1905.     CrossRef
  • Membrane Vesicles Are the Dominant Structural Components of Ceftazidime-Induced Biofilm Formation in an Oxacillin-Sensitive MRSA
    Xinlong He, Shuang Li, Yi Yin, Jiahui Xu, Weijuan Gong, Guocai Li, Li Qian, Yinyan Yin, Xiaoqin He, Tingting Guo, Yuzheng Huang, Feng Lu, Jun Cao
    Frontiers in Microbiology.2019;[Epub]     CrossRef
  • Biofilm formation byStaphylococcus aureusclinical isolates correlates with the infection type
    Jakub M. Kwiecinski, Gunnar Jacobsson, Alexander R. Horswill, Elisabet Josefsson, Tao Jin
    Infectious Diseases.2019; 51(6): 446.     CrossRef
  • Natural Products That Target Virulence Factors in Antibiotic-Resistant Staphylococcus aureus
    Shuai-Cheng Wu, Fei Liu, Kui Zhu, Jian-Zhong Shen
    Journal of Agricultural and Food Chemistry.2019; 67(48): 13195.     CrossRef
  • Antimicrobial resistance and genetic characterization of coagulase-negative staphylococci from bovine mastitis milk samples in Korea
    Su-Jeong Kim, Dong Chan Moon, Seung-Chun Park, Hee Young Kang, Seok Hyeon Na, Suk-Kyung Lim
    Journal of Dairy Science.2019; 102(12): 11439.     CrossRef
  • Options and Limitations in Clinical Investigation of Bacterial Biofilms
    Maria Magana, Christina Sereti, Anastasios Ioannidis, Courtney A. Mitchell, Anthony R. Ball, Emmanouil Magiorkinis, Stylianos Chatzipanagiotou, Michael R. Hamblin, Maria Hadjifrangiskou, George P. Tegos
    Clinical Microbiology Reviews.2018;[Epub]     CrossRef
  • Fighting biofilms with lantibiotics and other groups of bacteriocins
    Harsh Mathur, Des Field, Mary C. Rea, Paul D. Cotter, Colin Hill, R. Paul Ross
    npj Biofilms and Microbiomes.2018;[Epub]     CrossRef
  • Prevalence of Panton-Valentine Leukocidin Gene among Community AcquiredStaphylococcus aureus: A Real-Time PCR Study
    Amit Karmakar, Debarati Jana, Kunal Dutta, Parimal Dua, Chandradipa Ghosh
    Journal of Pathogens.2018; 2018: 1.     CrossRef
  • Biofilm Forming Ability and Spa Gene Polymorphism in Methicillin Resistant Staphylococcus aureus Clinical Isolates in North of Iran
    L. Asadpour
    Molecular Genetics, Microbiology and Virology.2018; 33(1): 55.     CrossRef
  • Clinical and Genetic Risk Factors for Biofilm-Forming Staphylococcus aureus
    Megan K. Luther, Diane M. Parente, Aisling R. Caffrey, Kathryn E. Daffinee, Vrishali V. Lopes, Emily T. Martin, Kerry L. LaPlante
    Antimicrobial Agents and Chemotherapy.2018;[Epub]     CrossRef
  • Comparative study of virulence factors among methicillin resistant Staphylococcus aureus clinical isolates
    Ons Haddad, Abderrahmen Merghni, Aida Elargoubi, Hajer Rhim, Yosr Kadri, Maha Mastouri
    BMC Infectious Diseases.2018;[Epub]     CrossRef
  • Microbacterium telephonicum sp. nov., isolated from the screen of a cellular phone
    Praveen Rahi, Rashmi Kurli, Aabeejjeet N. Pansare, Mitesh Khairnar, Shubhangi Jagtap, Nisha B. Patel, Syed G. Dastager, Paul A. Lawson, Yogesh S. Shouche
    International Journal of Systematic and Evolutiona.2018; 68(4): 1052.     CrossRef
  • Evaluation of Biofilm Formation and Presence of <italic>Ica</italic> Genes in <italic>Staphylococcus epidermidis</italic> Clinical Isolates
    Maryam Kord, Abdollah Ardebili, Maryam Jamalan, Roghaye Jahanbakhsh, Naser Behnampour, Ezzat Allah Ghaemi
    Osong Public Health and Research Perspectives.2018; 9(4): 160.     CrossRef
  • Utilization of supercritical carbon dioxide in fabrication of cellulose acetate films with anti-biofilm effects against Pseudomonas aeruginosa and Staphylococcus aureus
    Irena Zizovic, Lidija Senerovic, Ivana Moric, Tijana Adamovic, Milena Jovanovic, Melina Kalagasidis Krusic, Dusan Misic, Dusica Stojanovic, Stoja Milovanovic
    The Journal of Supercritical Fluids.2018; 140: 11.     CrossRef
  • Desiccation and ethanol resistances of multidrug resistant Acinetobacter baumannii embedded in biofilm: The favorable antiseptic efficacy of combination chlorhexidine gluconate and ethanol
    Shyh-Ren Chiang, Fang Jung, Hung-Jen Tang, Chung-Hua Chen, Chi-Chung Chen, Hsiu-Yin Chou, Yin-Ching Chuang
    Journal of Microbiology, Immunology and Infection.2018; 51(6): 770.     CrossRef
  • Biofilm Formation and Its Relationship with the Molecular Characteristics of Food‐Related Methicillin‐Resistant Staphylococcus aureus (MRSA)
    Alberto Vergara, Giovanni Normanno, Pierluigi Di Ciccio, Francesca Pedonese, Roberta Nuvoloni, Antonio Parisi, Gianfranco Santagada, Angelo Colagiorgi, Emanuela Zanardi, Sergio Ghidini, Adriana Ianieri
    Journal of Food Science.2017; 82(10): 2364.     CrossRef
  • MRSA decolonization failure—are biofilms the missing link?
    Frank Günther, Brigitte Blessing, Evelina Tacconelli, Nico T. Mutters
    Antimicrobial Resistance & Infection Control.2017;[Epub]     CrossRef
  • The role of biofilms in persistent infections and factors involved inica-independent biofilm development and gene regulation inStaphylococcus aureus
    Agnes Marie Sá Figueiredo, Fabienne Antunes Ferreira, Cristiana Ossaille Beltrame, Marina Farrel Côrtes
    Critical Reviews in Microbiology.2017; 43(5): 602.     CrossRef
  • Chlorhexidine whole-body washing of patients reduces methicillin-resistant Staphylococcus aureus and has a direct effect on the distribution of the ST5-MRSA-II (New York/Japan) clone
    Maria Elena Velázquez-Meza, Soraya Mendoza-Olazarán, Gabriela Echániz-Aviles, Adrián Camacho-Ortiz, Michel Fernando Martínez-Reséndez, Vanessa Valero-Moreno, Elvira Garza-González
    Journal of Medical Microbiology.2017; 66(6): 721.     CrossRef
  • Bovine origin Staphylococcus aureus: A new zoonotic agent?
    Relangi Tulasi Rao, Kannan Jayakumar, Pavitra Kumar
    Veterinary World.2017; 10(10): 1275.     CrossRef
  • Affinity interactions drive post-implantation drug filling, even in the presence of bacterial biofilm
    Erika L. Cyphert, Sean T. Zuckerman, Julius N. Korley, Horst A. von Recum
    Acta Biomaterialia.2017; 57: 95.     CrossRef
  • Emerging technologies for long-term antimicrobial device coatings: advantages and limitations
    Erika L Cyphert, Horst A von Recum
    Experimental Biology and Medicine.2017; 242(8): 788.     CrossRef
  • Methicillin-resistant food-related Staphylococcus aureus: a review of current knowledge and biofilm formation for future studies and applications
    Agapi I. Doulgeraki, Pierluigi Di Ciccio, Adriana Ianieri, George-John E. Nychas
    Research in Microbiology.2017; 168(1): 1.     CrossRef
  • Biofilm formation of Brazilian meticillin-resistant Staphylococcus aureus strains: prevalence of biofilm determinants and clonal profiles
    Deivid William da Fonseca Batistão, Paola Amaral de Campos, Nayara Caroline Camilo, Sabrina Royer, Bruna Fuga Araújo, Karinne Spirandelli Carvalho Naves, Margarida Martins, Maria Olívia Pereira, Mariana Henriques, Paulo Pinto Gontijo-Filho, Cláudia Botelh
    Journal of Medical Microbiology.2016; 65(4): 286.     CrossRef
  • Efficacy of Linezolid and Fosfomycin in Catheter-Related Biofilm Infection Caused by Methicillin-ResistantStaphylococcus aureus
    Dong Chai, Xu Liu, Rui Wang, Yan Bai, Yun Cai
    BioMed Research International.2016; 2016: 1.     CrossRef
  • Methicillin-resistant Staphylococcus aureus biofilm formation on dacryocystorhinostomy silicone tubes depends on the genetic lineage
    Ivana Ćirković, Miroslav Knežević, Dragana D. Božić, Dejan Rašić, Anders Rhod Larsen, Slobodanka Đukić
    Graefe's Archive for Clinical and Experimental Oph.2015; 253(1): 77.     CrossRef
  • Prevalence and Antimicrobial-Resistance of Staphylococcus aureus Isolated from Blood Culture in University Hospital, Turkey
    K Murat
    Global Journal of Infectious Diseases and Clinical.2015; : 010.     CrossRef

PHRP : Osong Public Health and Research Perspectives