Objectives Monkeypox outbreaks in nonendemic countries have been reported since early May 2022. The first case of monkeypox in the Republic of Korea was confirmed in a patient who traveled to Europe in June 2022, and an attempt was made to isolate and identify the monkeypox virus (MPXV) from the patient’s specimens.
Methods Clinical specimens from the patient were inoculated in Vero E6 cells. The isolated virus was identified as MPXV by the observation of cytopathic effects on Vero E6 cells, transmission electron microscopy, conventional polymerase chain reaction
(PCR), and sequencing of PCR products.
Results Cytopathic effects were observed in Vero E6 cells that were inoculated with skin lesion swab eluates. After multiple passages from the primary culture, orthopoxvirus morphology was observed using transmission electron microscopy. In addition, both MPXV-specific (F3L and ATI) and orthopoxvirus-specific genes (A39R, B2R, and HA) were confirmed by conventional PCR and Sanger sequencing.
Conclusion These results indicate the successful isolation and identification of MPXV from the first patient in the Republic of Korea. The isolated virus was named MPXV-ROK-P1-2022.
Citations
Citations to this article as recorded by
Epidemiological, Clinical, and Virological Investigation of the First Four Cases of Monkeypox in Cartagena during the 2022 Outbreak Steev Loyola, Mashiel Fernández-Ruiz, Doris Gómez-Camargo Pathogens.2023; 12(2): 159. CrossRef
원숭이두창바이러스의 분리 배양과 전장유전체 정보 분석 민지 이, 진원 김, 치환 최, 화철 신, 명민 최, 상은 이, 화중 이, 윤석 정 Public Health Weekly Report.2023; 16(15): 464. CrossRef
Overview of Diagnostic Methods, Disease Prevalence and Transmission of Mpox (Formerly Monkeypox) in Humans and Animal Reservoirs Ravendra P. Chauhan, Ronen Fogel, Janice Limson Microorganisms.2023; 11(5): 1186. CrossRef
How to cope with suspected mpox patients in the outpatient clinic Nam Joong Kim, Sun Huh Journal of the Korean Medical Association.2023; 66(5): 325. CrossRef
An Updated Review on Monkeypox Viral Disease: Emphasis on Genomic Diversity Ali A. Rabaan, Nada A. Alasiri, Mohammed Aljeldah, Abeer N. Alshukairiis, Zainab AlMusa, Wadha A. Alfouzan, Abdulmonem A. Abuzaid, Aref A. Alamri, Hani M. Al-Afghani, Nadira Al-baghli, Nawal Alqahtani, Nadia Al-baghli, Mashahed Y. Almoutawa, Maha Mahmoud Biomedicines.2023; 11(7): 1832. CrossRef
Monkeypox (Mpox) virus isolation and ultrastructural characterisation from a Brazilian human sample case Milene Dias Miranda, Gabriela Cardoso Caldas, Vivian Neuza Ferreira, Ortrud Monika Barth, Aline de Paula Dias da Silva, Mayara Secco Torres Silva, Beatriz Grinsztejn, Valdiléa Gonçalves Veloso, Thiago Moreno Souza, Edson Elias da Silva, Debora Ferreira Ba Memórias do Instituto Oswaldo Cruz.2023;[Epub] CrossRef
Isolation and Characterization of Monkeypox Virus from the First Case of Monkeypox — Chongqing Municipality, China, 2022 Baoying Huang, Hua Zhao, Jingdong Song, Li Zhao, Yao Deng, Wen Wang, Roujian Lu, Wenling Wang, Jiao Ren, Fei Ye, Houwen Tian, Guizhen Wu, Hua Ling, Wenjie Tan China CDC Weekly.2022; 4(46): 1019. CrossRef