Search
- Page Path
- HOME > Search
Brief Report
- Isolation and identification of monkeypox virus MPXV-ROK-P1-2022 from the first case in the Republic of Korea
- Jin-Won Kim, Minji Lee, Hwachul Shin, Chi-Hwan Choi, Myung-Min Choi, Jee Woong Kim, Hwajung Yi, Cheon-Kwon Yoo, Gi-Eun Rhie
- Osong Public Health Res Perspect. 2022;13(4):308-311. Published online August 31, 2022
- DOI: https://doi.org/10.24171/j.phrp.2022.0232
- 2,486 View
- 109 Download
- 4 Citations
-
Graphical Abstract
Abstract
PDF 
- Objectives
Monkeypox outbreaks in nonendemic countries have been reported since early May 2022. The first case of monkeypox in the Republic of Korea was confirmed in a patient who traveled to Europe in June 2022, and an attempt was made to isolate and identify the monkeypox virus (MPXV) from the patient’s specimens.
Methods
Clinical specimens from the patient were inoculated in Vero E6 cells. The isolated virus was identified as MPXV by the observation of cytopathic effects on Vero E6 cells, transmission electron microscopy, conventional polymerase chain reaction (PCR), and sequencing of PCR products.
Results
Cytopathic effects were observed in Vero E6 cells that were inoculated with skin lesion swab eluates. After multiple passages from the primary culture, orthopoxvirus morphology was observed using transmission electron microscopy. In addition, both MPXV-specific (F3L and ATI) and orthopoxvirus-specific genes (A39R, B2R, and HA) were confirmed by conventional PCR and Sanger sequencing.
Conclusion
These results indicate the successful isolation and identification of MPXV from the first patient in the Republic of Korea. The isolated virus was named MPXV-ROK-P1-2022. -
Citations
Citations to this article as recorded by
- Epidemiological, Clinical, and Virological Investigation of the First Four Cases of Monkeypox in Cartagena during the 2022 Outbreak
Steev Loyola, Mashiel Fernández-Ruiz, Doris Gómez-Camargo
Pathogens.2023; 12(2): 159. CrossRef - 원숭이두창바이러스의 분리 배양과 전장유전체 정보 분석
민지 이, 진원 김, 치환 최, 화철 신, 명민 최, 상은 이, 화중 이, 윤석 정
Public Health Weekly Report.2023; 16(15): 464. CrossRef - Overview of Diagnostic Methods, Disease Prevalence and Transmission of Mpox (Formerly Monkeypox) in Humans and Animal Reservoirs
Ravendra P. Chauhan, Ronen Fogel, Janice Limson
Microorganisms.2023; 11(5): 1186. CrossRef - Isolation and Characterization of Monkeypox Virus from the First Case of Monkeypox — Chongqing Municipality, China, 2022
Baoying Huang, Hua Zhao, Jingdong Song, Li Zhao, Yao Deng, Wen Wang, Roujian Lu, Wenling Wang, Jiao Ren, Fei Ye, Houwen Tian, Guizhen Wu, Hua Ling, Wenjie Tan
China CDC Weekly.2022; 4(46): 1019. CrossRef
- Epidemiological, Clinical, and Virological Investigation of the First Four Cases of Monkeypox in Cartagena during the 2022 Outbreak
First
Prev
