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Alterations of gyrA, gyrB, and parC and Activity of Efflux Pump in Fluoroquinolone-resistant Acinetobacter baumannii
Sunok Park, Kyeong Min Lee, Yong Sun Yoo, Jung Sik Yoo, Jae Il Yoo, Hwa Su Kim, Yeong Seon Lee, Gyung Tae Chung
Osong Public Health Res Perspect. 2011;2(3):164-170.   Published online December 31, 2011
DOI: https://doi.org/10.1016/j.phrp.2011.11.040
  • 1,984 View
  • 15 Download
  • 29 Citations
AbstractAbstract PDF
Objectives
This study investigated the fluoroquinolone-resistant mechanism of 56 clinical cases of A baumannii infection from 23 non-tertiary hospitals, collected between 2004 and 2006.
Methods
Susceptibility testing was performed by broth microdilution and Epsilometer test. Analyses of quinolone resistance-determining region (QRDR) were done by sequencing. The activity of the efflux pump was measured using inhibitors.
Results
The sequences from selected 56 isolates were divided into seven groups (I-VII) on the basis of mutations in gyrA (S83L), parC (S80L, S80W and S84K) and gyrB (containing the novel mutations E679D, D644Y and A677V). The 27 isolates with triple mutations in gyrA, gyrB and parC (groups IV-VII) showed higher levels of resistance to ciprofloxacin (minimal inhibitory concentration [MIC] of 16-256 μg/mL) than the 26 isolates with double mutations in gyrA and parC (groups II and III, MIC of 8-64 μ g/mL; p < 0.05). Alterations in the efflux pump were observed in four isolates with the parC S80L mutation (group II) or E84K mutation (group VII), but no effect was observed in an isolate with the parC S80 W mutation (group III).
Conclusion
These results suggest that triple mutations in clinical isolates of A baumannii contribute to the development of high levels of resistance to fluoroquinolones and that mutations in parC S80L or E84K (groups II and VII) may contribute to alterations in efflux pump activity in A baumannii.

Citations

Citations to this article as recorded by  
  • Development of Nanoparticle Adaptation Phenomena in Acinetobacter baumannii: Physiological Change and Defense Response
    Oliver McNeilly, Riti Mann, Max Laurence Cummins, Steven P. Djordjevic, Mehrad Hamidian, Cindy Gunawan, Victor Gonzalez
    Microbiology Spectrum.2023;[Epub]     CrossRef
  • Colistin potentiation in multidrug-resistant Acinetobacter baumannii by a non-cytotoxic guanidine derivative of silver
    Deepak Kumar, Chaitali Singhal, Manisha Yadav, Pooja Joshi, Priyanka Patra, Subhash Tanwar, Amitava Das, Sumit Kumar Pramanik, Susmita Chaudhuri
    Frontiers in Microbiology.2023;[Epub]     CrossRef
  • A comprehensive review on potential therapeutic inhibitors of nosocomial Acinetobacter baumannii superbugs
    Danaboina Srikanth, Swanand Vinayak Joshi, Mahammad Ghouse Shaik, Gaurav Pawar, Sushmitha Bujji, Vinaykumar Kanchupalli, Sidharth Chopra, Srinivas Nanduri
    Bioorganic Chemistry.2022; 124: 105849.     CrossRef
  • Differential Binding of Carbapenems with the AdeABC Efflux Pump and Modulation of the Expression of AdeB Linked to Novel Mutations within Two-Component System AdeRS in Carbapenem-Resistant Acinetobacter baumannii
    Subhasree Roy, Vivek Junghare, Shanta Dutta, Saugata Hazra, Sulagna Basu, Christopher W. Marshall
    mSystems.2022;[Epub]     CrossRef
  • Antimicrobial Resistance, Integron Carriage, and Fluoroquinolone Resistance Genes in Acinetobacte baumannii Isolates
    Parastoo Ashouri, Jafar Mohammadshahi, Vajihe Sadat Nikbin, Hadi Peeridogaheh, Behnam Mohammadi-Ghalehbin, Soheila Refahi, Amir Teimourpour, Majid Esmaelizad, Hafez Mirzaneghad, Roghayeh Teimourpour
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    Subhasree Roy, Somdatta Chatterjee, Amrita Bhattacharjee, Pinaki Chattopadhyay, Bijan Saha, Shanta Dutta, Sulagna Basu
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  • Shared and Unique Evolutionary Trajectories to Ciprofloxacin Resistance in Gram-Negative Bacterial Pathogens
    Jaime E. Zlamal, Semen A. Leyn, Mallika Iyer, Marinela L. Elane, Nicholas A. Wong, James W. Wamsley, Maarten Vercruysse, Fernando Garcia-Alcalde, Andrei L. Osterman, Igor B. Zhulin
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  • Efflux Pump Activity and Mutations Driving Multidrug Resistance in Acinetobacter baumannii at a Tertiary Hospital in Pretoria, South Africa
    Noel-David Nogbou, Granny M. Nkawane, Khanyisa Ntshane, Charles K. Wairuri, Dikwata T. Phofa, Kagiso K. Mokgokong, Mbudzeni Ramashia, Maphoshane Nchabeleng, Lawrence C. Obi, Andrew M. Musyoki, Todd R. Callaway
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  • The effect of the efflux pump inhibitor Carbonyl Cyanide m-Chlorophenylhydrazone (CCCP) on the susceptibility to imipenem and cefepime in clinical strains of Acinetobacter baumannii
    Alejandra Sanchez-Carbonel, Belén Mondragón, Nicolás López-Chegne, Isaac Peña-Tuesta, Gladys Huayan-Dávila, Dora Blitchtein, Hugo Carrillo-Ng, Wilmer Silva-Caso, Miguel Angel Aguilar-Luis, Juana del Valle-Mendoza, Iddya Karunasagar
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  • Antibiotic Resistance Profiles, Molecular Mechanisms and Innovative Treatment Strategies of Acinetobacter baumannii
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  • Bacterial Genome Wide Association Studies (bGWAS) and Transcriptomics Identifies Cryptic Antimicrobial Resistance Mechanisms in Acinetobacter baumannii
    Chandler Roe, Charles H. D. Williamson, Adam J. Vazquez, Kristen Kyger, Michael Valentine, Jolene R. Bowers, Paul D. Phillips, Veronica Harrison, Elizabeth Driebe, David M. Engelthaler, Jason W. Sahl
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  • Molecular Analyses of Biofilm-Producing Clinical Acinetobacter baumannii Isolates from a South Indian Tertiary Care Hospital
    Balaram Khamari, Manmath Lama, Chanakya Pachi Pulusu, Amarendra Pratap  Biswal, Sai Manoz Lingamallu, Bhargava Sai Mukkirla, Amit Kumar Sahoo, Harioum Sambhu Narayan Dash, Rewa Sharda, Prakash Kumar, Eswarappa Pradeep Bulagonda
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    Roshan D'Souza, Naina A. Pinto, Nguyen Le Phuong, Paul G. Higgins, Thao Nguyen Vu, Jung-Hyun Byun, Young Lag Cho, Jong Rak Choi, Dongeun Yong
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  • Molecular Study of Quinolone Resistance Determining Regions of gyrA Gene and parC Genes in Clinical Isolates of Acintobacter baumannii Resistant to Fluoroquinolone
    Maysaa El Sayed Zaki, Nermen Abou ElKheir, Mohamed Mofreh
    The Open Microbiology Journal.2018; 12(1): 116.     CrossRef
  • Species distribution, virulence factors and antimicrobial resistance ofAcinetobacterspp. isolates from dogs and cats: a preliminary study
    Yui Kimura, Kazuki Harada, Takae Shimizu, Tomomi Sato, Akari Kajino, Masaru Usui, Yutaka Tamura, Yuzo Tsuyuki, Tadashi Miyamoto, Asami Ohki, Masahisa Watarai
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  • Multiple mechanisms contributing to ciprofloxacin resistance among Gram negative bacteria causing infections to cancer patients
    Samira M. Hamed, Walid F. Elkhatib, Hadir A. El-Mahallawy, Mai M. Helmy, Mohamed S. Ashour, Khaled M. A. Aboshanab
    Scientific Reports.2018;[Epub]     CrossRef
  • Synthesis and investigation of binding interactions of 1,4-benzoxazine derivatives on topoisomerase IV in Acinetobacter baumannii
    S. Yilmaz, I. Yalcin, S. Okten, F. K. Onurdag, E. Aki-Yalcin
    SAR and QSAR in Environmental Research.2017; 28(11): 941.     CrossRef
  • Antibacterial Resistance in Ureaplasma Species and Mycoplasma hominis Isolates from Urine Cultures in College-Aged Females
    Marissa A. Valentine-King, Mary B. Brown
    Antimicrobial Agents and Chemotherapy.2017;[Epub]     CrossRef
  • MOLECULAR BACKGROUND OF FLUOROQUINOLONE RESISTANCE IN PATHOGENIC HUMAN MYCOPLASMAS
    A. N. Vaganova
    Russian Journal of Infection and Immunity.2017; 7(3): 231.     CrossRef
  • Alarmingly High Segregation Frequencies of Quinolone Resistance Alleles within Human and Animal Microbiomes Are Not Explained by Direct Clinical Antibiotic Exposure
    Wesley Field, Ruth Hershberg
    Genome Biology and Evolution.2015; 7(6): 1743.     CrossRef
  • Multidrug-resistant Acinetobactor baumannii isolated from a traveler returned from Brunei
    Masayoshi Tojo, Momoko Mawatari, Kayoko Hayakawa, Maki Nagamatsu, Kayo Shimada, Kazuhisa Mezaki, Yuko Sugiki, Emi Kuroda, Nozomi Takeshita, Satoshi Kutsuna, Yoshihiro Fujiya, Tohru Miyoshi-Akiyama, Teruo Kirikae, Norio Ohmagari
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  • Correlation of Ciprofloxacin Resistance with the AdeABC Efflux System in Acinetobacter baumannii Clinical Isolates
    Abdollah Ardebili, Abdolaziz Rastegar Lari, Malihe Talebi
    Annals of Laboratory Medicine.2014; 34(6): 433.     CrossRef
  • Effect of Efflux Pump Inhibitor Carbonyl Cyanide 3-Chlorophenylhydrazone on the Minimum Inhibitory Concentration of Ciprofloxacin in Acinetobacter baumannii Clinical Isolates
    Abdollah Ardebili, Malihe Talebi, Leila Azimi, Abdolaziz Rastegar Lari
    Jundishapur Journal of Microbiology.2014;[Epub]     CrossRef
  • Characterization of Acinetobacter baumannii Clinical Isolates Carrying blaOXA-23 Carbapenemase and 16S rRNA Methylase armA genes in Yemen
    Sofiane Bakour, Samer Ahmed Alsharapy, Abdelaziz Touati, Jean-Marc Rolain
    Microbial Drug Resistance.2014; 20(6): 604.     CrossRef
  • First report of 16S rRNA methylase ArmA-producing Acinetobacter baumannii and rapid spread of metallo-β-lactamase NDM-1 in Algerian hospitals
    Sofiane Bakour, Abdelaziz Touati, Taous Bachiri, Farida Sahli, Djamel Tiouit, Malek Naim, Mounia Azouaou, Jean-Marc Rolain
    Journal of Infection and Chemotherapy.2014; 20(11): 696.     CrossRef
Original Article
Gene Expression and Identification Related to Fluconazole Resistance of Candida glabrata Strains
Jae Il Yoo, Chi Won Choi, Kyeong Min Lee, Yeong Seon Lee
Osong Public Health Res Perspect. 2010;1(1):36-41.   Published online December 31, 2010
DOI: https://doi.org/10.1016/j.phrp.2010.12.009
  • 1,941 View
  • 15 Download
  • 8 Citations
AbstractAbstract PDF
Objectives
Candida glabrata has become one of the most common causes of Candida bloodstream infections worldwide. Some strains of C. glabrata may be intermediately resistant to all azoles. The several possible mechanisms of azole resistance have been reported previously, but the exact resistant mechanism is not clear. In this study, we identified differentially expressed genes (DEGs) of fluconazole-resistant C. glabrata and compared the gene expression of fluconazole-resistant strains with that of fluconazole-susceptible strains to identify gene corresponding to fluconazole resistance.
Methods
Using antifungal susceptibility test, several C. glabrata strains were selected and used for further study. The expression of CgCDR1 and CgCDR2 genes was investigated by slot hybridization against fluconazole-susceptible, -resistant, and resistant-induced strains. In addition, ERG3 and ERG11 genes were sequenced to analyze DNA base substitution. DEGs were identified by reverse transcription-polymerase chain reaction using DEG kit composed of 120 random primers.
Results
In slot hybridization, CgCDR1 gene was expressed more than CgCDR2 gene in resistant strains. Though base substitution of ERG11 and ERG3 genes was observed in several base sequences, just one amino acid change was identified in resistant strain. In the results of reverse transcription-polymerase chain reaction, 44 genes were upregulated and 34 genes were downregulated. Among them, adenosine triphosphate-binding cassette transporter-related genes, fatty acid desaturase, lyase, and hypothetical protein genes were upregulated and aldehyde dehydrogenase, oxidoreductase, and prohibitin-like protein genes were downregulated. Other DEGs were also identified.
Conclusion
This study showed that CgCDR1 gene was more closely related to fluconazole resistance of C. glabrata than CgCDR2 gene. In addition, several other genes related with fluconazole resistance of C. glabrata were identified.

Citations

Citations to this article as recorded by  
  • Candida glabrata: Pathogenicity and Resistance Mechanisms for Adaptation and Survival
    Yahaya Hassan, Shu Yih Chew, Leslie Thian Lung Than
    Journal of Fungi.2021; 7(8): 667.     CrossRef
  • Candidiasis and Mechanisms of Antifungal Resistance
    Somanon Bhattacharya, Sutthichai Sae-Tia, Bettina C. Fries
    Antibiotics.2020; 9(6): 312.     CrossRef
  • A Transcriptomics Approach To Unveiling the Mechanisms of In Vitro Evolution towards Fluconazole Resistance of a Candida glabrata Clinical Isolate
    Mafalda Cavalheiro, Catarina Costa, Ana Silva-Dias, Isabel M. Miranda, Can Wang, Pedro Pais, Sandra N. Pinto, Dalila Mil-Homens, Michiyo Sato-Okamoto, Azusa Takahashi-Nakaguchi, Raquel M. Silva, Nuno P. Mira, Arsénio M. Fialho, Hiroji Chibana, Acácio G. R
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    Berdieke Goemaere, Katrien Lagrou, Isabel Spriet, Marijke Hendrickx, Pierre Becker
    Antimicrobial Agents and Chemotherapy.2018;[Epub]     CrossRef
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    Charlene Wilma Joyce Africa, Pedro Miguel dos Santos Abrantes
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    Atefeh Abdollahi Gohar, Hamid Badali, Tahereh Shokohi, Mojtaba Nabili, Nasrin Amirrajab, Maryam Moazeni
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    Mojtaba Nabili, Maryam Moazeni, Mohammad Taghi Hedayati, Parisa Aryamlo, Atefeh Abdollahi Gohar, Seyed Mehdi Madani, Hamed Fathi
    Journal of Global Antimicrobial Resistance.2017; 11: 52.     CrossRef
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    Charlene Wilma Joyce Africa, Pedro Miguel dos Santos Abrantes
    F1000Research.2016; 5: 2832.     CrossRef

PHRP : Osong Public Health and Research Perspectives