Objectives Monkeypox outbreaks in nonendemic countries have been reported since early May 2022. The first case of monkeypox in the Republic of Korea was confirmed in a patient who traveled to Europe in June 2022, and an attempt was made to isolate and identify the monkeypox virus (MPXV) from the patient’s specimens.
Methods Clinical specimens from the patient were inoculated in Vero E6 cells. The isolated virus was identified as MPXV by the observation of cytopathic effects on Vero E6 cells, transmission electron microscopy, conventional polymerase chain reaction
(PCR), and sequencing of PCR products.
Results Cytopathic effects were observed in Vero E6 cells that were inoculated with skin lesion swab eluates. After multiple passages from the primary culture, orthopoxvirus morphology was observed using transmission electron microscopy. In addition, both MPXV-specific (F3L and ATI) and orthopoxvirus-specific genes (A39R, B2R, and HA) were confirmed by conventional PCR and Sanger sequencing.
Conclusion These results indicate the successful isolation and identification of MPXV from the first patient in the Republic of Korea. The isolated virus was named MPXV-ROK-P1-2022.
Citations
Citations to this article as recorded by
MPXV: Update on Morphological and Morphogenesis Aspects Through Transmission and Scanning Electron Microscopies and 3D Reconstruction Debora Ferreira Barreto‐Vieira, Milene Dias Miranda, Marcos Alexandre Nunes da Silva, Andressa Santos de Almeida, Ana Luisa Teixeira de Almeida, Derick Mendes Bandeira, Vivian Neuza S. Ferreira, Alice Santos Rosa, Wendell Girard‐Dias, Bráulio Soares Archa Journal of Medical Virology.2025;[Epub] CrossRef
Ultrasensitive one-pot detection of monkeypox virus with RPA and CRISPR in a sucrose-aided multiphase aqueous system Yue Wang, Yixin Tang, Yukang Chen, Guangxi Yu, Xue Zhang, Lihong Yang, Chenjie Zhao, Pei Wang, Song Gao, Frederick S. B. Kibenge, Ruijie Deng, Wei Chen, Shuang Yang Microbiology Spectrum.2024;[Epub] CrossRef
Molecular Methods for Diagnosis of Monkeypox: A Mini-review Rodrigo Michelini de Oliveira Thomasi, Thais da Silva Correa, Dalise Silva do Carmo, Déborah Fernandes Rodrigues, Luiz Vinicius da Silva Correa, Sandra Rodrigues Xavier, Líria Souza Silva, Jonatas Oliveira da Silva, Michelli dos Santos, Alessandra da Silv Current Molecular Medicine.2024; 24(10): 1208. CrossRef
Factors Predicting the Intention to Engage in Self-Protective Behavior against Monkeypox Infection among Thai People Awirut Singkun, Wichan Phibarn, Wichaporn Intharachuti, Arunothai Derramon, Thitika Kimise Journal of Health Research.2024;[Epub] CrossRef
Epidemiological, Clinical, and Virological Investigation of the First Four Cases of Monkeypox in Cartagena during the 2022 Outbreak Steev Loyola, Mashiel Fernández-Ruiz, Doris Gómez-Camargo Pathogens.2023; 12(2): 159. CrossRef
원숭이두창바이러스의 분리 배양과 전장유전체 정보 분석 민지 이, 진원 김, 치환 최, 화철 신, 명민 최, 상은 이, 화중 이, 윤석 정 Public Health Weekly Report.2023; 16(15): 464. CrossRef
Overview of Diagnostic Methods, Disease Prevalence and Transmission of Mpox (Formerly Monkeypox) in Humans and Animal Reservoirs Ravendra P. Chauhan, Ronen Fogel, Janice Limson Microorganisms.2023; 11(5): 1186. CrossRef
How to cope with suspected mpox patients in the outpatient clinic Nam Joong Kim, Sun Huh Journal of the Korean Medical Association.2023; 66(5): 325. CrossRef
An Updated Review on Monkeypox Viral Disease: Emphasis on Genomic Diversity Ali Rabaan, Nada Alasiri, Mohammed Aljeldah, Abeer Alshukairiis, Zainab AlMusa, Wadha Alfouzan, Abdulmonem Abuzaid, Aref Alamri, Hani Al-Afghani, Nadira Al-baghli, Nawal Alqahtani, Nadia Al-baghli, Mashahed Almoutawa, Maha Mahmoud Alawi, Mohammed Alabdull Biomedicines.2023; 11(7): 1832. CrossRef
Monkeypox (Mpox) virus isolation and ultrastructural characterisation from a Brazilian human sample case Milene Dias Miranda, Gabriela Cardoso Caldas, Vivian Neuza Ferreira, Ortrud Monika Barth, Aline de Paula Dias da Silva, Mayara Secco Torres Silva, Beatriz Grinsztejn, Valdiléa Gonçalves Veloso, Thiago Moreno Souza, Edson Elias da Silva, Debora Ferreira Ba Memórias do Instituto Oswaldo Cruz.2023;[Epub] CrossRef
Isolation and Characterization of Monkeypox Virus from the First Case of Monkeypox — Chongqing Municipality, China, 2022 Baoying Huang, Hua Zhao, Jingdong Song, Li Zhao, Yao Deng, Wen Wang, Roujian Lu, Wenling Wang, Jiao Ren, Fei Ye, Houwen Tian, Guizhen Wu, Hua Ling, Wenjie Tan China CDC Weekly.2022; 4(46): 1019. CrossRef
Il-Hwan Kim, Jun Hyeong Jang, Su-Kyoung Jo, Jin Sun No, Seung-Hee Seo, Jun-Young Kim, Sang-Oun Jung, Jeong-Min Kim, Sang-Eun Lee, Hye-Kyung Park, Eun-Jin Kim, Jun Ho Jeon, Myung-Min Choi, Boyeong Ryu, Yoon Suk Jang, Hwami Kim, Jin Lee, Seung-Hwan Shin, Hee Kyoung Kim, Eun-Kyoung Kim, Ye Eun Park, Cheon-Kwon Yoo, Sang-Won Lee, Myung-Guk Han, Gi-Eun Rhie, Byung Hak Kang
Osong Public Health Res Perspect 2020;11(5):280-285. Published online October 22, 2020
<sec>
<title>Objectives</title>
<p>The Korea Centers for Disease Control and Prevention has published “A Guideline for Unknown Disease Outbreaks (UDO).” The aim of this report was to introduce tabletop exercises (TTX) to prepare for UDO in the future.</p></sec>
<sec>
<title>Methods</title>
<p>The UDO Laboratory Analyses Task Force in Korea Centers for Disease Control and Prevention in April 2018, assigned unknown diseases into 5 syndromes, designed an algorithm for diagnosis, and made a panel list for diagnosis by exclusion. Using the guidelines and laboratory analyses for UDO, TTX were introduced.</p></sec>
<sec>
<title>Results</title>
<p>Since September 9<sup>th</sup>, 2018, the UDO Laboratory Analyses Task Force has been preparing TTX based on a scenario of an outbreak caused by a novel coronavirus. In December 2019, through TTX, individual missions, epidemiological investigations, sample treatments, diagnosis by exclusions, and next generation sequencing analysis were discussed, and a novel coronavirus was identified as the causal pathogen.</p></sec>
<sec>
<title>Conclusion</title>
<p>Guideline and laboratory analyses for UDO successfully applied in TTX. Conclusions drawn from TTX could be applied effectively in the analyses for the initial response to COVID-19, an ongoing epidemic of 2019 – 2020. Therefore, TTX should continuously be conducted for the response and preparation against UDO.</p></sec>
Citations
Citations to this article as recorded by
Use of a pathogen X tabletop exercise to assess the operational response preparedness of an emerging infectious diseases research network Rachael Lee, Jennifer Hemingway-Foday, Nefer Batsuli, L. Danielle Wagner, Aaron Macoubray, Robert F. Garry, Christine K. Johnson, Kathryn A. Hanley, Nikos Vasilakis, Souleymane Mboup, Hongying Li, Cecilia A. Sánchez, Peter M. Rabinowitz, Robert F. Breiman Frontiers in Public Health.2025;[Epub] CrossRef
Development and evaluation of the Korea Disease Control and Prevention Agency’s scenario-based training program for effective response to emerging infectious disease outbreaks Mi Yu, Eun Kyoung Kim, Kyungnam Kim, Jin Lee, Jin Hwan Jeon, Soyeon Kim, Sangwon Lee, Yunhyung Kwon Osong Public Health and Research Perspectives.2025; 16(4): 373. CrossRef
Development and evaluation of a hybrid capture-based NGS panel for comprehensive detection of respiratory pathogens Jeong-Ah Kim, Jeong-Min Kim, Chaeyoung Lee, Il-Hwan Kim, Daehwan Lee, Heesoo Lee, Jaehwan Jeong, Eun-Jin Kim Scientific Reports.2025;[Epub] CrossRef
Education Needs for Public Health Crisis Using an Importance-Performance Analysis and Borich Needs Assessment Model: Focused on Aircraft Quarantine Officials Jiyun Park, Gye Joung Yeom, Seok Hwan Kim Research in Community and Public Health Nursing.2025; 36: 361. CrossRef
First
Prev
