Skip Navigation
Skip to contents

PHRP : Osong Public Health and Research Perspectives

OPEN ACCESS
SEARCH
Search

Search

Page Path
HOME > Search
2 "Identification"
Filter
Filter
Article category
Keywords
Publication year
Authors
Funded articles
Brief Report
Isolation and identification of monkeypox virus MPXV-ROK-P1-2022 from the first case in the Republic of Korea
Jin-Won Kim, Minji Lee, Hwachul Shin, Chi-Hwan Choi, Myung-Min Choi, Jee Woong Kim, Hwajung Yi, Cheon-Kwon Yoo, Gi-Eun Rhie
Osong Public Health Res Perspect. 2022;13(4):308-311.   Published online August 31, 2022
DOI: https://doi.org/10.24171/j.phrp.2022.0232
  • 1,754 View
  • 87 Download
  • 2 Citations
Graphical AbstractGraphical Abstract AbstractAbstract PDF
Objectives
Monkeypox outbreaks in nonendemic countries have been reported since early May 2022. The first case of monkeypox in the Republic of Korea was confirmed in a patient who traveled to Europe in June 2022, and an attempt was made to isolate and identify the monkeypox virus (MPXV) from the patient’s specimens.
Methods
Clinical specimens from the patient were inoculated in Vero E6 cells. The isolated virus was identified as MPXV by the observation of cytopathic effects on Vero E6 cells, transmission electron microscopy, conventional polymerase chain reaction (PCR), and sequencing of PCR products.
Results
Cytopathic effects were observed in Vero E6 cells that were inoculated with skin lesion swab eluates. After multiple passages from the primary culture, orthopoxvirus morphology was observed using transmission electron microscopy. In addition, both MPXV-specific (F3L and ATI) and orthopoxvirus-specific genes (A39R, B2R, and HA) were confirmed by conventional PCR and Sanger sequencing.
Conclusion
These results indicate the successful isolation and identification of MPXV from the first patient in the Republic of Korea. The isolated virus was named MPXV-ROK-P1-2022.

Citations

Citations to this article as recorded by  
  • Epidemiological, Clinical, and Virological Investigation of the First Four Cases of Monkeypox in Cartagena during the 2022 Outbreak
    Steev Loyola, Mashiel Fernández-Ruiz, Doris Gómez-Camargo
    Pathogens.2023; 12(2): 159.     CrossRef
  • Isolation and Characterization of Monkeypox Virus from the First Case of Monkeypox — Chongqing Municipality, China, 2022
    Baoying Huang, Hua Zhao, Jingdong Song, Li Zhao, Yao Deng, Wen Wang, Roujian Lu, Wenling Wang, Jiao Ren, Fei Ye, Houwen Tian, Guizhen Wu, Hua Ling, Wenjie Tan
    China CDC Weekly.2022; 4(46): 1019.     CrossRef
Original Article
Gene Expression and Identification Related to Fluconazole Resistance of Candida glabrata Strains
Jae Il Yoo, Chi Won Choi, Kyeong Min Lee, Yeong Seon Lee
Osong Public Health Res Perspect. 2010;1(1):36-41.   Published online December 31, 2010
DOI: https://doi.org/10.1016/j.phrp.2010.12.009
  • 1,941 View
  • 15 Download
  • 8 Citations
AbstractAbstract PDF
Objectives
Candida glabrata has become one of the most common causes of Candida bloodstream infections worldwide. Some strains of C. glabrata may be intermediately resistant to all azoles. The several possible mechanisms of azole resistance have been reported previously, but the exact resistant mechanism is not clear. In this study, we identified differentially expressed genes (DEGs) of fluconazole-resistant C. glabrata and compared the gene expression of fluconazole-resistant strains with that of fluconazole-susceptible strains to identify gene corresponding to fluconazole resistance.
Methods
Using antifungal susceptibility test, several C. glabrata strains were selected and used for further study. The expression of CgCDR1 and CgCDR2 genes was investigated by slot hybridization against fluconazole-susceptible, -resistant, and resistant-induced strains. In addition, ERG3 and ERG11 genes were sequenced to analyze DNA base substitution. DEGs were identified by reverse transcription-polymerase chain reaction using DEG kit composed of 120 random primers.
Results
In slot hybridization, CgCDR1 gene was expressed more than CgCDR2 gene in resistant strains. Though base substitution of ERG11 and ERG3 genes was observed in several base sequences, just one amino acid change was identified in resistant strain. In the results of reverse transcription-polymerase chain reaction, 44 genes were upregulated and 34 genes were downregulated. Among them, adenosine triphosphate-binding cassette transporter-related genes, fatty acid desaturase, lyase, and hypothetical protein genes were upregulated and aldehyde dehydrogenase, oxidoreductase, and prohibitin-like protein genes were downregulated. Other DEGs were also identified.
Conclusion
This study showed that CgCDR1 gene was more closely related to fluconazole resistance of C. glabrata than CgCDR2 gene. In addition, several other genes related with fluconazole resistance of C. glabrata were identified.

Citations

Citations to this article as recorded by  
  • Candida glabrata: Pathogenicity and Resistance Mechanisms for Adaptation and Survival
    Yahaya Hassan, Shu Yih Chew, Leslie Thian Lung Than
    Journal of Fungi.2021; 7(8): 667.     CrossRef
  • Candidiasis and Mechanisms of Antifungal Resistance
    Somanon Bhattacharya, Sutthichai Sae-Tia, Bettina C. Fries
    Antibiotics.2020; 9(6): 312.     CrossRef
  • A Transcriptomics Approach To Unveiling the Mechanisms of In Vitro Evolution towards Fluconazole Resistance of a Candida glabrata Clinical Isolate
    Mafalda Cavalheiro, Catarina Costa, Ana Silva-Dias, Isabel M. Miranda, Can Wang, Pedro Pais, Sandra N. Pinto, Dalila Mil-Homens, Michiyo Sato-Okamoto, Azusa Takahashi-Nakaguchi, Raquel M. Silva, Nuno P. Mira, Arsénio M. Fialho, Hiroji Chibana, Acácio G. R
    Antimicrobial Agents and Chemotherapy.2019;[Epub]     CrossRef
  • Clonal Spread of Candida glabrata Bloodstream Isolates and Fluconazole Resistance Affected by Prolonged Exposure: a 12-Year Single-Center Study in Belgium
    Berdieke Goemaere, Katrien Lagrou, Isabel Spriet, Marijke Hendrickx, Pierre Becker
    Antimicrobial Agents and Chemotherapy.2018;[Epub]     CrossRef
  • Candida antifungal drug resistance in sub-Saharan African populations: A systematic review
    Charlene Wilma Joyce Africa, Pedro Miguel dos Santos Abrantes
    F1000Research.2017; 5: 2832.     CrossRef
  • Expression Patterns of ABC Transporter Genes in Fluconazole-Resistant Candida glabrata
    Atefeh Abdollahi Gohar, Hamid Badali, Tahereh Shokohi, Mojtaba Nabili, Nasrin Amirrajab, Maryam Moazeni
    Mycopathologia.2017; 182(3-4): 273.     CrossRef
  • Glabridin induces overexpression of two major apoptotic genes, MCA1 and NUC1 , in Candida albicans
    Mojtaba Nabili, Maryam Moazeni, Mohammad Taghi Hedayati, Parisa Aryamlo, Atefeh Abdollahi Gohar, Seyed Mehdi Madani, Hamed Fathi
    Journal of Global Antimicrobial Resistance.2017; 11: 52.     CrossRef
  • Candida antifungal drug resistance in sub-Saharan African populations: A systematic review
    Charlene Wilma Joyce Africa, Pedro Miguel dos Santos Abrantes
    F1000Research.2016; 5: 2832.     CrossRef

PHRP : Osong Public Health and Research Perspectives