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Original Article
- Gene Expression and Identification Related to Fluconazole Resistance of
Candida glabrata Strains - Jae Il Yoo, Chi Won Choi, Kyeong Min Lee, Yeong Seon Lee
- Osong Public Health Res Perspect. 2010;1(1):36-41. Published online December 31, 2010
- DOI: https://doi.org/10.1016/j.phrp.2010.12.009
- 2,759 View
- 16 Download
- 9 Crossref
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Abstract
PDF - Objectives
Candida glabrata has become one of the most common causes of Candida bloodstream infections worldwide. Some strains of C. glabrata may be intermediately resistant to all azoles. The several possible mechanisms of azole resistance have been reported previously, but the exact resistant mechanism is not clear. In this study, we identified differentially expressed genes (DEGs) of fluconazole-resistant C. glabrata and compared the gene expression of fluconazole-resistant strains with that of fluconazole-susceptible strains to identify gene corresponding to fluconazole resistance.
Methods
Using antifungal susceptibility test, several C. glabrata strains were selected and used for further study. The expression of CgCDR1 and CgCDR2 genes was investigated by slot hybridization against fluconazole-susceptible, -resistant, and resistant-induced strains. In addition, ERG3 and ERG11 genes were sequenced to analyze DNA base substitution. DEGs were identified by reverse transcription-polymerase chain reaction using DEG kit composed of 120 random primers.
Results
In slot hybridization, CgCDR1 gene was expressed more than CgCDR2 gene in resistant strains. Though base substitution of ERG11 and ERG3 genes was observed in several base sequences, just one amino acid change was identified in resistant strain. In the results of reverse transcription-polymerase chain reaction, 44 genes were upregulated and 34 genes were downregulated. Among them, adenosine triphosphate-binding cassette transporter-related genes, fatty acid desaturase, lyase, and hypothetical protein genes were upregulated and aldehyde dehydrogenase, oxidoreductase, and prohibitin-like protein genes were downregulated. Other DEGs were also identified.
Conclusion
This study showed that CgCDR1 gene was more closely related to fluconazole resistance of C. glabrata than CgCDR2 gene. In addition, several other genes related with fluconazole resistance of C. glabrata were identified. -
Citations
Citations to this article as recorded by
- Molecular Mechanisms Associated with Antifungal Resistance in Pathogenic Candida Species
Karolina M. Czajka, Krishnan Venkataraman, Danielle Brabant-Kirwan, Stacey A. Santi, Chris Verschoor, Vasu D. Appanna, Ravi Singh, Deborah P. Saunders, Sujeenthar Tharmalingam
Cells.2023; 12(22): 2655. CrossRef - Candida glabrata: Pathogenicity and Resistance Mechanisms for Adaptation and Survival
Yahaya Hassan, Shu Yih Chew, Leslie Thian Lung Than
Journal of Fungi.2021; 7(8): 667. CrossRef - Candidiasis and Mechanisms of Antifungal Resistance
Somanon Bhattacharya, Sutthichai Sae-Tia, Bettina C. Fries
Antibiotics.2020; 9(6): 312. CrossRef -
A Transcriptomics Approach To Unveiling the Mechanisms of
In Vitro
Evolution towards Fluconazole Resistance of a
Candida glabrata
Clinical Isolate
Mafalda Cavalheiro, Catarina Costa, Ana Silva-Dias, Isabel M. Miranda, Can Wang, Pedro Pais, Sandra N. Pinto, Dalila Mil-Homens, Michiyo Sato-Okamoto, Azusa Takahashi-Nakaguchi, Raquel M. Silva, Nuno P. Mira, Arsénio M. Fialho, Hiroji Chibana, Acácio G. R
Antimicrobial Agents and Chemotherapy.2019;[Epub] CrossRef - Clonal Spread of Candida glabrata Bloodstream Isolates and Fluconazole Resistance Affected by Prolonged Exposure: a 12-Year Single-Center Study in Belgium
Berdieke Goemaere, Katrien Lagrou, Isabel Spriet, Marijke Hendrickx, Pierre Becker
Antimicrobial Agents and Chemotherapy.2018;[Epub] CrossRef - Candida antifungal drug resistance in sub-Saharan African populations: A systematic review
Charlene Wilma Joyce Africa, Pedro Miguel dos Santos Abrantes
F1000Research.2017; 5: 2832. CrossRef - Expression Patterns of ABC Transporter Genes in Fluconazole-Resistant Candida glabrata
Atefeh Abdollahi Gohar, Hamid Badali, Tahereh Shokohi, Mojtaba Nabili, Nasrin Amirrajab, Maryam Moazeni
Mycopathologia.2017; 182(3-4): 273. CrossRef - Glabridin induces overexpression of two major apoptotic genes, MCA1 and NUC1 , in Candida albicans
Mojtaba Nabili, Maryam Moazeni, Mohammad Taghi Hedayati, Parisa Aryamlo, Atefeh Abdollahi Gohar, Seyed Mehdi Madani, Hamed Fathi
Journal of Global Antimicrobial Resistance.2017; 11: 52. CrossRef - Candida antifungal drug resistance in sub-Saharan African populations: A systematic review
Charlene Wilma Joyce Africa, Pedro Miguel dos Santos Abrantes
F1000Research.2016; 5: 2832. CrossRef
- Molecular Mechanisms Associated with Antifungal Resistance in Pathogenic Candida Species
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