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2 "phylogeny"
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Original Articles
Characterization of Antimicrobial Susceptibility, Extended-Spectrum β-Lactamase Genes and Phylogenetic Groups of Enteropathogenic Escherichia coli Isolated from Patients with Diarrhea
Erfaneh Jafari, Saeid Mostaan, Saeid Bouzari
Osong Public Health Res Perspect. 2020;11(5):327-333.   Published online October 22, 2020
DOI: https://doi.org/10.24171/j.phrp.2020.11.5.09
  • 5,337 View
  • 85 Download
  • 4 Citations
AbstractAbstract PDF
Objectives

Infectious diarrhea is one of the most common causes of pediatric death worldwide and enteropathogenic Escherichia coli (EPEC) is one of the main causes. There are 2 subgroups of EPEC, typical and atypical, based on the presence or absence of bundle forming pili (bfp), of which atypical EPEC is considered less virulent, but not less pathogenic. Antimicrobial resistance towards atypical EPEC among children is growing and is considered a major problem. In this study the pattern of antibiotic resistance in clinical isolates was determined.

Methods

Using 130 isolates, antibiotic resistance patterns and phenotypes were assessed, and genotypic profiles of extended spectrum β-lactamase (ESBL) production using disc diffusion and PCR was carried out. Phylogenetic groups were analyzed using quadruplex PCR.

Results

There were 65 E. coli isolates identified as atypical EPEC by PCR, among which the highest antibiotic resistance was towards ampicillin, followed by trimethoprim-sulfamethoxazole, and tetracycline. Multidrug resistance was detected in 44.6% of atypical EPEC isolates. Around 33% of isolates were determined to be extended spectrum β-lactamase producers, and in 90% of isolates, genes responsible for ESBL production could be detected. Moreover, the majority of atypical EPEC strains belonged to Group E, followed by Groups B1, B2 and C.

Conclusion

High rates of multidrug resistance and ESBL production among atypical EPEC isolates warrant periodical surveillance studies to select effective antibiotic treatment for patients. It is considered a critical step to manage antibiotic resistance by avoiding unnecessary prescriptions for antibiotics.

Citations

Citations to this article as recorded by  
  • Virulence and phylogenetic analysis of enteric pathogenic Escherichia coli isolated from children with diarrhoea in South Africa
    Ntando W. Alfinete, John Y. Bolukaoto, Lee Heine, Natasha Potgieter, Tobias G. Barnard
    International Journal of Infectious Diseases.2022; 114: 226.     CrossRef
  • Prevalence of extended-spectrum β-lactamases, AmpC, and carbapenemases in Proteus mirabilis clinical isolates
    Mona Shaaban, Soha Lotfy Elshaer, Ola A. Abd El-Rahman
    BMC Microbiology.2022;[Epub]     CrossRef
  • Enteropathogenic Escherichia coli—A Summary of the Literature
    Anca Delia Mare, Cristina Nicoleta Ciurea, Adrian Man, Bianca Tudor, Valeriu Moldovan, Luminița Decean, Felicia Toma
    Gastroenterology Insights.2021; 12(1): 28.     CrossRef
  • Prevalence of Antibiotic-Resistant Escherichia coli Isolated from Swine Faeces and Lagoons in Bulgaria
    Lyudmila Dimitrova, Mila Kaleva, Maya M. Zaharieva, Christina Stoykova, Iva Tsvetkova, Maya Angelovska, Yana Ilieva, Vesselin Kussovski, Sevda Naydenska, Hristo Najdenski
    Antibiotics.2021; 10(8): 940.     CrossRef
Epidemiological Characteristics of Field Tick-Borne Pathogens in Gwang-ju Metropolitan Area, South Korea, from 2014 to 2018
Jung Wook Park, Seung Hun Lee, Gi Seong Lee, Jin Jong Seo, Jae Keun Chung
Osong Public Health Res Perspect. 2020;11(4):177-184.   Published online August 31, 2020
DOI: https://doi.org/10.24171/j.phrp.2020.11.4.06
  • 4,154 View
  • 86 Download
  • 4 Citations
AbstractAbstract PDF
Objectives

The importance of tick-borne diseases is increasing because of climate change, with a lack of long-term studies on tick-borne pathogens in South Korea. To understand the epidemiological characteristics of tick-borne diseases, the monthly distribution of field ticks throughout the year was studied in South Korea between May 2014 and April 2018 in a cross sectional study.

Methods

The presence of various tick-borne pathogens (Rickettsia species, Borrelia species, Anaplasma phagocytophilum) was confirmed by using polymerase chain reaction, to provide information for a prevention strategy against tick-borne pathogenic infections, through increased understanding of the relationship between seasonal variation and risk of infection with Rickettsia species. This was performed using logistic regression analysis (SPSS 20, IBM, USA) of the data obtained from the study.

Results

During the study period there were 11,717 ticks collected and 4 species identified. Haemapysalis longicornis was the most common species (n = 10,904, 93.1%), followed by Haemapysalis flava (n = 656, 5.6%), Ixodes nipponensis (n = 151, 1.3%), and Amblyomma testudinarium (n = 6, 0.05%) The results of this cross-sectional study showed that Haemapysalis flava carried a higher risk of transmission of Rickettsia species than other tick species (p < 0.05).

Conclusion

In conclusion, due attention should be paid to preventing tick-borne infections in humans whilst engaged in outdoor activities in Spring and Autumn, particularly in places where there is a high prevalence of ticks.

Citations

Citations to this article as recorded by  
  • Molecular Detection of Anaplasma phagocytophilum and Ehrlichia Species in Ticks Removed from Humans in the Republic of Korea
    Yu-Jung Kim, Ji Ye Seo, Seong Yoon Kim, Hee Il Lee
    Microorganisms.2022; 10(6): 1224.     CrossRef
  • Molecular Detection and Phylogeny of Tick-Borne Pathogens in Ticks Collected from Dogs in the Republic of Korea
    A-Tai Truong, Jinhyeong Noh, Yeojin Park, Hyun-Ji Seo, Keun-Ho Kim, Subin Min, Jiyeon Lim, Mi-Sun Yoo, Heung-Chul Kim, Terry A. Klein, Hyunkyoung Lee, Soon-Seek Yoon, Yun Sang Cho
    Pathogens.2021; 10(5): 613.     CrossRef
  • iSeq 100 for metagenomic pathogen screening in ticks
    Ju Yeong Kim, Myung-hee Yi, Alghurabi Areej Sabri Mahdi, Tai-Soon Yong
    Parasites & Vectors.2021;[Epub]     CrossRef
  • Molecular Identification of Borrelia spp. from Ticks in Pastures Nearby Livestock Farms in Korea
    Haeseung Lee, Seung-Hun Lee, SungShik Shin, Dongmi Kwak
    Insects.2021; 12(11): 1011.     CrossRef

PHRP : Osong Public Health and Research Perspectives