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2 "reverse transcription-polymerase chain reaction"
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Original Article
One-Step Reverse Transcription-Polymerase Chain Reaction for Ebola and Marburg Viruses
Sun-Whan Park, Ye-Ji Lee, Won-Ja Lee, Youngmee Jee, WooYoung Choi
Osong Public Health Res Perspect. 2016;7(3):205-209.   Published online June 30, 2016
DOI: https://doi.org/10.1016/j.phrp.2016.04.004
  • 2,949 View
  • 25 Download
  • 5 Crossref
AbstractAbstract PDF
Objectives
Ebola and Marburg viruses (EBOVs and MARVs, respectively) are causative agents of severe hemorrhagic fever with high mortality rates in humans and nonhuman primates. In 2014, there was a major Ebola outbreak in various countries in West Africa, including Guinea, Liberia, Republic of Sierra Leone, and Nigeria. EBOV and MARV are clinically difficult to diagnose and distinguish from other African epidemic diseases. Therefore, in this study, we aimed to develop a method for rapid identification of the virus to prevent the spread of infection.
Methods
We established a conventional one-step reverse transcription-polymerase chain reaction (RT-PCR) assay for these pathogens based on the Superscript Reverse Transcriptase-Platinum Taq polymerase enzyme mixture. All assays were thoroughly optimized using in vitro-transcribed RNA.
Results
We designed seven primer sets of nucleocapsid protein (NP) genes based on sequences from seven filoviruses, including five EBOVs and two MARVs. To evaluate the sensitivity of the RT-PCR assay for each filovirus, 10-fold serial dilutions of synthetic viral RNA transcripts of EBOV or MARV NP genes were used to assess detection limits of viral RNA copies. The potential for these primers to cross react with other filoviruses was also examined. The results showed that the primers were specific for individual genotype detection in the examined filoviruses.
Conclusion
The assay established in this study may facilitate rapid, reliable laboratory diagnosis in suspected cases of Ebola and Marburg hemorrhagic fevers.

Citations

Citations to this article as recorded by  
  • Marburg Virus Disease – A Mini-Review
    Sandip Chakraborty, Deepak Chandran, Ranjan K. Mohapatra, Mahmoud Alagawany, Mohd Iqbal Yatoo, Md. Aminul Islam, Anil K. Sharma, Kuldeep Dhama
    Journal of Experimental Biology and Agricultural S.2022; 10(4): 689.     CrossRef
  • Marburgviruses: An Update
    Caterina M Miraglia
    Laboratory Medicine.2019; 50(1): 16.     CrossRef
  • Ebola virus: A global public health menace: A narrative review
    Shamimul Hasan, SyedAnsar Ahmad, Rahnuma Masood, Shazina Saeed
    Journal of Family Medicine and Primary Care.2019; 8(7): 2189.     CrossRef
  • Fast and Parallel Detection of Four Ebola Virus Species on a Microfluidic-Chip-Based Portable Reverse Transcription Loop-Mediated Isothermal Amplification System
    Xue Lin, Xiangyu Jin, Bin Xu, Ruliang Wang, Rongxin Fu, Ya Su, Kai Jiang, Han Yang, Ying Lu, Yong Guo, Guoliang Huang
    Micromachines.2019; 10(11): 777.     CrossRef
  • The current landscape of nucleic acid tests for filovirus detection
    David J. Clark, John Tyson, Andrew D. Sails, Sanjeev Krishna, Henry M. Staines
    Journal of Clinical Virology.2018; 103: 27.     CrossRef
Review Article
Travel-Associated Chikungunya Cases in South Korea during 2009–2010
Go Woon Cha, Jung Eun Cho, Eun Ju Lee, Young Ran Ju, Myung Guk Han, Chan Park, Young Eui Jeong
Osong Public Health Res Perspect. 2013;4(3):170-175.   Published online June 30, 2013
DOI: https://doi.org/10.1016/j.phrp.2013.04.008
  • 3,198 View
  • 16 Download
  • 10 Crossref
AbstractAbstract PDF
Objectives
Chikungunya (CHIK) has been classified as a communicable disease group IV in South Korea since late 2010. Based on this, we investigated the extent of imported cases of CHIK in dengue-suspected individuals returning from dengue-endemic regions.
Methods
A total of 486 dengue-suspected serum samples were screened for CHIK by enzyme-linked immunosorbent assay (ELISA) and reverse transcription-polymerase chain reaction (RT-PCR) analysis. Further RT-PCR-positive samples were used for the viral culture, and CHIK was subsequently confirmed by sequence analysis of the culture samples.
Results
Five out of 107 dengue-positive samples were found to be positive for CHIK and 15 out of 379 dengue-negative samples were found to be positive for CHIK by immunoglobulin M ELISA. Further, a CHIK virus was isolated from one of the two RT-PCR-positive sera by cell culture and confirmed by sequence analysis.
Conclusion
The present study documents the first evidence of travel-associated CHIK infection in South Korea. Considering the intense international traffic between countries, our finding emphasizes the urgent need for active patient and vector surveillance for timely response to reduce the introduction of CHIK in Korea.

Citations

Citations to this article as recorded by  
  • Global prevalence of dengue and chikungunya coinfection: A systematic review and meta-analysis of 43,341 participants
    Ahmad Adebayo Irekeola, E.A. R Engku Nur Syafirah, Md Asiful Islam, Rafidah Hanim Shueb
    Acta Tropica.2022; 231: 106408.     CrossRef
  • Current Status and a Perspective of Mosquito-Borne Diseases in the Republic of Korea
    Jae Hyoung Im, Tong-Soo Kim, Moon-Hyun Chung, Ji Hyeon Baek, Hea Yoon Kwon, Jin-Soo Lee
    Vector-Borne and Zoonotic Diseases.2021; 21(2): 69.     CrossRef
  • Implications of a travel connectivity-based approach for infectious disease transmission risks in Oceania
    Angela Cadavid Restrepo, Luis Furuya-Kanamori, Helen Mayfield, Eric Nilles, Colleen L Lau
    BMJ Open.2021; 11(8): e046206.     CrossRef
  • Development of a neutralization assay based on the pseudotyped chikungunya virus of a Korean isolate
    Woo-Chang Chung, Kwang Yeon Hwang, Suk-Jo Kang, Jae-Ouk Kim, Moon Jung Song
    Journal of Microbiology.2020; 58(1): 46.     CrossRef
  • Chikungunya virus infection in Indonesia: a systematic review and evolutionary analysis
    Harapan Harapan, Alice Michie, Mudatsir Mudatsir, Roy Nusa, Benediktus Yohan, Abram Luther Wagner, R. Tedjo Sasmono, Allison Imrie
    BMC Infectious Diseases.2019;[Epub]     CrossRef
  • Global prevalence and distribution of coinfection of malaria, dengue and chikungunya: a systematic review
    Nasir Salam, Shoeb Mustafa, Abdul Hafiz, Anis Ahmad Chaudhary, Farah Deeba, Shama Parveen
    BMC Public Health.2018;[Epub]     CrossRef
  • Chikungunya Virus Infection after Traveling to Surinam, South America
    Hoe-Soo Jang, Jong-Hun Chung, Joa Kim, Sun Ae Han, Na-Ra Yun, Dong-Min Kim
    The Korean Journal of Medicine.2016; 90(3): 262.     CrossRef
  • Molecular epidemiology, evolution and phylogeny of Chikungunya virus: An updating review
    Alessandra Lo Presti, Eleonora Cella, Silvia Angeletti, Massimo Ciccozzi
    Infection, Genetics and Evolution.2016; 41: 270.     CrossRef
  • The First Imported Case Infected with Chikungunya Virus in Korea
    Jeong-Hwan Hwang, Chang-Seop Lee
    Infection & Chemotherapy.2015; 47(1): 55.     CrossRef
  • Zika virus in Brazil and the danger of infestation by Aedes (Stegomyia) mosquitoes
    Carlos Brisola Marcondes, Maria de Fátima Freire de Melo Ximenes
    Revista da Sociedade Brasileira de Medicina Tropic.2015; 49(1): 4.     CrossRef

PHRP : Osong Public Health and Research Perspectives