Osong Public Health and Research Perspectives

Original Article

Cloning and Expression of Recombinant Tick-Borne Encephalitis Virus-like Particles in Pichia pastoris: Seok-Min Yun, Young Eui Jeong, Eunbyeol Wang, Ye-Ji Lee, Myung Guk Han, Chan Park, Won-Ja Lee, WooYoung Choi; Osong Public Health Res Perspect. 2014;5(5):274-278. Published online October 31, 2014; DOI: https://doi.org/10.1016/j.phrp.2014.08.005

3,183 View
16 Download
9 Crossref

Objectives
The purpose of this study was to verify the feasibility of using the glyceraldehyde-3-phosphate dehydrogenase (GAP) promotor based Pichia pastoris expression system to produce tick-borne encephalitis virus (TBEV) virus-like particles (VLPs).
Methods
The complementary DNA encoding the TBEV prM signal peptide, prM, and E proteins of TBEV Korean strain (KrM 93) was cloned into the plasmid vector pGAPZɑA, then integrated into the genome of P. pastoris, under the control of the GAP promoter. Expression of TBEV VLPs was determined by Western blotting using monoclonal antibody against TBEV envelope (E) protein.
Results
Recombinant TBEV VLPs consisting of prM and E protein were successfully expressed using the GAP promoter-based P. pastoris expression system. The results of Western blotting showed that the recombinant proteins were secreted into the culture supernatant from the P. pastoris and glycosylated.
Conclusion
This study suggests that recombinant TBEV VLPs from P. pastoris offer a promising approach to the production of VLPs for use as vaccines and diagnostic antigens.

Citations

Citations to this article as recorded by

Yeast-Based Virus-like Particles as an Emerging Platform for Vaccine Development and Delivery
Vartika Srivastava, Kripa N. Nand, Aijaz Ahmad, Ravinder Kumar
Vaccines.2023; 11(2): 479. CrossRef
Yeast and Virus-like Particles: A Perfect or Imperfect Couple?
Sara Brachelente, Alvaro Galli, Tiziana Cervelli
Applied Microbiology.2023; 3(3): 805. CrossRef
De novo transcriptome sequencing and comparative profiling of the ovary in partially engorged and fully engorged Haemaphysalis flava ticks
Yu Zhao, Zhe-Hui Qu, Feng-Chao Jiao
Parasitology International.2021; 83: 102344. CrossRef
Flavivirus vaccines: Virus-like particles and single-round infectious particles as promising alternatives
Esmeralda Cuevas-Juárez, Victoria Pando-Robles, Laura A. Palomares
Vaccine.2021; 39(48): 6990. CrossRef
NS1 Recombinant Proteins Are Efficiently Produced in Pichia pastoris and Have Great Potential for Use in Diagnostic Kits for Dengue Virus Infections
Mariana Fonseca Xisto, John Willians Oliveira Prates, Ingrid Marques Dias, Roberto Sousa Dias, Cynthia Canedo da Silva, Sérgio Oliveira de Paula
Diagnostics.2020; 10(6): 379. CrossRef
Tick-Borne Encephalitis Virus: A Quest for Better Vaccines against a Virus on the Rise
Mareike Kubinski, Jana Beicht, Thomas Gerlach, Asisa Volz, Gerd Sutter, Guus F. Rimmelzwaan
Vaccines.2020; 8(3): 451. CrossRef
Virus-Like Particle Systems for Vaccine Development Against Viruses in the Flaviviridae Family
Wong, Jassey, Wang, Wang, Liu, Lin
Vaccines.2019; 7(4): 123. CrossRef
ON MODERN APPROACHES TO CREATION OF A SINGLE-CYCLE VACCINE AGAINST TICK-BORNE ENCEPHALITIS
V. A. Lashkevich, G. G. Karganova
Problems of Virology.2018; 63(3): 101. CrossRef
Production of an enzymatically active and immunogenic form of ectodomain of Porcine rubulavirus hemagglutinin-neuraminidase in the yeast Pichia pastoris
José Luis Cerriteño-Sánchez, Gerardo Santos-López, Nora Hilda Rosas-Murrieta, Julio Reyes-Leyva, Sandra Cuevas-Romero, Irma Herrera-Camacho
Journal of Biotechnology.2016; 223: 52. CrossRef

Article

Prevalence of Tick-Borne Encephalitis Virus in Ixodid Ticks Collected from the Republic of Korea During 2011–2012: Seok-Min Yun, Bong Gu Song, WooYoung Choi, Won Il Park, Sung Yun Kim, Jong Yul Roh, Jungsang Ryou, Young Ran Ju, Chan Park, E-Hyun Shin; Osong Public Health Res Perspect. 2012;3(4):213-221. Published online December 31, 2012; DOI: https://doi.org/10.1016/j.phrp.2012.10.004

3,453 View
26 Download
28 Crossref

Abstract PDF

Objectives
In this study, we demonstrated that TBEV-infected ticks have been distributed in the ROK, combined with our previous results. These results suggest that TBEV may exist in the ROK, and H. longicornis, H. flava, and I. nipponensis may be potential vectors of TBEV. In addition, these results emphasize the need for further epidemiological research of TBEV.
Methods
We examined for the presence of RNA of TBEV by reverse transcriptase-nested polymerase chain reaction (RT-nested PCR) using ixodid ticks captured in 25 localities of 10 provinces. Ticks were collected by the flagging and dragging method or using sentinel BG traps at forests, grass thickets, and grassland. A total of 13,053 ticks belonging to two genera and four species were collected and pooled (1292 pools), according to collection site, species of tick, and developmental stage.
Results
Among 1292 pools, the envelope (E) protein gene of TBEV was detected using RT-nested PCR in 10 pools (3 pools of the 1,331 adult ticks and 7 pools of the 11,169 nymph ticks) collected from Gangwon-do province, Jeonrabuk-do province, and Jeju Island. The minimum infection rates for TBEV of Haemaphysalis longicornis, Haemaphysalis flava, and Ixodes nipponensis were 0.06%, 0.17%, and 2.38%, respectively. Phylogenetic analysis based on the partial E protein gene was performed to identify relationships between the TBEV strains. This showed that 10 Korean strains clustered with the Western subtype.
Conclusion
In this study, we investigated the prevalence of tick-borne encephalitis virus (TBEV) in ixodid ticks from various regions of the Republic of Korea (ROK) during 2011–2012 to identify whether TBEV is circulating and to determine the endemic regions of TBEV.

Citations

Citations to this article as recorded by

TBE in South Korea
Joon Young Song
Tick-borne encephalitis - The Book.2023;[Epub] CrossRef
Molecular detection and phylogenetic analysis of tick‐borne encephalitis virus in ticks in northeastern China
Xiaohui Li, Hongwei Ji, Di Wang, Lihe Che, Li Zhang, Liang Li, Qing Yin, Quan Liu, Feng Wei, Zedong Wang
Journal of Medical Virology.2022; 94(2): 507. CrossRef
TBE in South Korea
Song Joon Young
Tick-borne encephalitis - The Book.2022;[Epub] CrossRef
Genomic Determinants Potentially Associated with Clinical Manifestations of Human-Pathogenic Tick-Borne Flaviviruses
Artem N. Bondaryuk, Nina V. Kulakova, Ulyana V. Potapova, Olga I. Belykh, Anzhelika V. Yudinceva, Yurij S. Bukin
International Journal of Molecular Sciences.2022; 23(21): 13404. CrossRef
TBE in South Korea
Song Joon Young
Tick-borne encephalitis - The Book.2021;[Epub] CrossRef
Hard Ticks as Vectors Tested Negative for Severe Fever with Thrombocytopenia Syndrome in Ganghwa-do, Korea during 2019-2020
Kyoung Jin, Yeon-Ja Koh, Seong Kyu Ahn, Joonghee Cho, Junghwan Lim, Jaeyong Song, Jinyoung Lee, Young Woo Gong, Mun Ju Kwon, Hyung Wook Kwon, Young Yil Bahk, Tong-Soo Kim
The Korean Journal of Parasitology.2021; 59(3): 281. CrossRef
Nationwide Temporal and Geographical Distribution of Tick Populations and Phylogenetic Analysis of Severe Fever with Thrombocytopenia Syndrome Virus in Ticks in Korea, 2020
Min-Goo Seo, Byung-Eon Noh, Hak Seon Lee, Tae-Kyu Kim, Bong-Goo Song, Hee Il Lee
Microorganisms.2021; 9(8): 1630. CrossRef
Seroepidemiologic survey of emerging vector-borne infections in South Korean forest/field workers
Ji Yun Noh, Joon Young Song, Joon Yong Bae, Man-Seong Park, Jin Gu Yoon, Hee Jin Cheong, Woo Joo Kim, Nam-Hyuk Cho
PLOS Neglected Tropical Diseases.2021; 15(8): e0009687. CrossRef
European subtype of tick-borne encephalitis virus. Literature review
Yu. S. Savinova
Acta Biomedica Scientifica.2021; 6(4): 100. CrossRef
Tick-Borne Encephalitis Virus: An Emerging Ancient Zoonosis?
Andrei A. Deviatkin, Ivan S. Kholodilov, Yulia A. Vakulenko, Galina G. Karganova, Alexander N. Lukashev
Viruses.2020; 12(2): 247. CrossRef
Characterization of tick-borne encephalitis virus isolated from a tick in central Hokkaido in 2017
Yuji Takahashi, Shintaro Kobayashi, Mariko Ishizuka, Minato Hirano, Memi Muto, Shoko Nishiyama, Hiroaki Kariwa, Kentaro Yoshii
Journal of General Virology.2020; 101(5): 497. CrossRef
A history of the introduction, establishment, dispersal and management ofHaemaphysalis longicornisNeumann, 1901 (Ixodida: Ixodidae) in New Zealand
Allen C. G. Heath
New Zealand Journal of Zoology.2020; 47(4): 241. CrossRef
Four Year Surveillance of the Vector Hard Ticks for SFTS, Ganghwa-do, Republic of Korea
Myung-Deok Kim-Jeon, Seung Jegal, Hojong Jun, Haneul Jung, Seo Hye Park, Seong Kyu Ahn, Jinyoung Lee, Young Woo Gong, Kwangsig Joo, Mun Ju Kwon, Jong Yul Roh, Wook-Gyo Lee, Young Yil Bahk, Tong-Soo Kim
The Korean Journal of Parasitology.2019; 57(6): 691. CrossRef
TBE in South Korea
Joon Young Song
Tick-borne encephalitis - The Book.2019;[Epub] CrossRef
Ixodid ticks and tick-borne encephalitis virus prevalence in the South Asian part of Russia (Republic of Tuva)
Ivan Kholodilov, Oxana Belova, Ludmila Burenkova, Yuri Korotkov, Lidiya Romanova, Lola Morozova, Vitalii Kudriavtsev, Larissa Gmyl, Ilmira Belyaletdinova, Alexander Chumakov, Natalia Chumakova, Oyumaa Dargyn, Nina Galatsevich, Anatoly Gmyl, Mikhail Mikhai
Ticks and Tick-borne Diseases.2019; 10(5): 959. CrossRef
Current Status of Tick-Borne Diseases in South Korea
Jae Hyoung Im, JiHyeon Baek, Areum Durey, Hea Yoon Kwon, Moon-Hyun Chung, Jin-Soo Lee
Vector-Borne and Zoonotic Diseases.2019; 19(4): 225. CrossRef
Molecular characterization of Haemaphysalis longicornis-borne rickettsiae, Republic of Korea and China
Ju Jiang, Huijuan An, John S. Lee, Monica L. O’Guinn, Heung-Chul Kim, Sung-Tae Chong, Yanmin Zhang, Dan Song, Roxanne G. Burrus, Yuzhou Bao, Terry A. Klein, Allen L. Richards
Ticks and Tick-borne Diseases.2018; 9(6): 1606. CrossRef
Molecular detection of Rickettsia species in ticks collected from the southwestern provinces of the Republic of Korea
Yoontae Noh, Yeong Seon Lee, Heung-Chul Kim, Sung-Tae Chong, Terry A. Klein, Ju Jiang, Allen L. Richards, Hae Kyeong Lee, Su Yeon Kim
Parasites & Vectors.2017;[Epub] CrossRef
Necessity of a Surveillance System for Tick-borne Encephalitis
Seok-Ju Yoo, Ji-Hyuk Park
Osong Public Health and Research Perspectives.2017; 8(2): 155. CrossRef
Tick-borne encephalitis in Japan, Republic of Korea and China
Kentaro Yoshii, Joon Young Song, Seong-Beom Park, Junfeng Yang, Heinz-Josef Schmitt
Emerging Microbes & Infections.2017; 6(1): 1. CrossRef
Molecular detection of severe fever with thrombocytopenia syndrome and tick-borne encephalitis viruses in ixodid ticks collected from vegetation, Republic of Korea, 2014
Seok-Min Yun, Ye-Ji Lee, WooYoung Choi, Heung-Chul Kim, Sung-Tae Chong, Kyu-Sik Chang, Jordan M. Coburn, Terry A. Klein, Won-Ja Lee
Ticks and Tick-borne Diseases.2016; 7(5): 970. CrossRef
The characterization of TBEV of European subtype circulating in Siberia, Russia
I. V. Kozlova, T. V. Demina, S. E. Tkachev, Yu. S. Savinova, E. K. Doroshchenko, O. V. Lisak, Yu. P. Dzhioev, O. V. Suntsova, M. M. Verkhozina, A. I. Paramonov, N. V. Tikunova, V. I. Zlobin, D. . Ruzek
Epidemiology and Vaccine Prevention.2016; 15(6): 30. CrossRef
Detection of SFTS Virus inIxodes nipponensisandAmblyomma testudinarium(Ixodida: Ixodidae) Collected From Reptiles in the Republic of Korea
Jae-Hwa Suh, Heung-Chul Kim, Seok-Min Yun, Jae-Won Lim, Jin-Han Kim, Sung-Tae Chong, Dae-Ho Kim, Hyun-Tae Kim, Hyun Kim, Terry A. Klein, Jaree L. Johnson, Won-Ja Lee
Journal of Medical Entomology.2016; 53(3): 584. CrossRef
Epidemiological Features and Clinical Manifestations of Lyme Borreliosis in Korea during the Period 2005^|^ndash;2012
Shinje Moon, Yeongseon Hong, Kyu-Jam Hwang, Suyeon Kim, Jihye Eom, Donghyok Kwon, Ji-Hyuk Park, Seung-Ki Youn, Aeree Sohn
Japanese Journal of Infectious Diseases.2015; 68(1): 1. CrossRef
Characteristics and Factors Associated with Death among Patients Hospitalized for Severe Fever with Thrombocytopenia Syndrome, South Korea, 2013
Jaeseung Shin, Donghyok Kwon, Seung-Ki Youn, Ji-Hyuk Park
Emerging Infectious Diseases.2015; 21(10): 1704. CrossRef
Review: Sentinels of tick-borne encephalitis risk
Maren Imhoff, Peter Hagedorn, Yesica Schulze, Wiebke Hellenbrand, Martin Pfeffer, Matthias Niedrig
Ticks and Tick-borne Diseases.2015; 6(5): 592. CrossRef
Louping ill virus (LIV) in the Far East
Galina N. Leonova, Ilya G. Kondratov, Olga S. Maystrovskaya, Ikuo Takashima, Sergei I. Belikov
Archives of Virology.2015; 160(3): 663. CrossRef
Prevalence of severe fever with thrombocytopenia syndrome virus in Haemaphysalis longicornis ticks in South Korea
Sun-Whan Park, Bong Gu Song, E-Hyun Shin, Seok-Min Yun, Myung-Guk Han, Mi Yeoun Park, Chan Park, Jungsang Ryou
Ticks and Tick-borne Diseases.2014; 5(6): 975. CrossRef

First
Prev
Page of 1
Next
Last

Search