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Original Article
- Cloning and Expression of Recombinant Tick-Borne Encephalitis Virus-like Particles in
Pichia pastoris - Seok-Min Yun, Young Eui Jeong, Eunbyeol Wang, Ye-Ji Lee, Myung Guk Han, Chan Park, Won-Ja Lee, WooYoung Choi
- Osong Public Health Res Perspect. 2014;5(5):274-278. Published online October 31, 2014
- DOI: https://doi.org/10.1016/j.phrp.2014.08.005
- 3,690 View
- 20 Download
- 10 Crossref
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Abstract
PDF - Objectives
The purpose of this study was to verify the feasibility of using the glyceraldehyde-3-phosphate dehydrogenase (GAP) promotor based Pichia pastoris expression system to produce tick-borne encephalitis virus (TBEV) virus-like particles (VLPs).
Methods
The complementary DNA encoding the TBEV prM signal peptide, prM, and E proteins of TBEV Korean strain (KrM 93) was cloned into the plasmid vector pGAPZɑA, then integrated into the genome of P. pastoris, under the control of the GAP promoter. Expression of TBEV VLPs was determined by Western blotting using monoclonal antibody against TBEV envelope (E) protein.
Results
Recombinant TBEV VLPs consisting of prM and E protein were successfully expressed using the GAP promoter-based P. pastoris expression system. The results of Western blotting showed that the recombinant proteins were secreted into the culture supernatant from the P. pastoris and glycosylated.
Conclusion
This study suggests that recombinant TBEV VLPs from P. pastoris offer a promising approach to the production of VLPs for use as vaccines and diagnostic antigens. -
Citations
Citations to this article as recorded by
- Influence of adjuvant type and route of administration on the immunogenicity of Leishmania-derived tick-borne encephalitis virus-like particles – A recombinant vaccine candidate
Marta Zimna, Gabriela Brzuska, Jiří Salát, Daniel Růžek, Ewelina Krol
Antiviral Research.2024; 228: 105941. CrossRef - Yeast-Based Virus-like Particles as an Emerging Platform for Vaccine Development and Delivery
Vartika Srivastava, Kripa N. Nand, Aijaz Ahmad, Ravinder Kumar
Vaccines.2023; 11(2): 479. CrossRef - Yeast and Virus-like Particles: A Perfect or Imperfect Couple?
Sara Brachelente, Alvaro Galli, Tiziana Cervelli
Applied Microbiology.2023; 3(3): 805. CrossRef - De novo transcriptome sequencing and comparative profiling of the ovary in partially engorged and fully engorged Haemaphysalis flava ticks
Yu Zhao, Zhe-Hui Qu, Feng-Chao Jiao
Parasitology International.2021; 83: 102344. CrossRef - Flavivirus vaccines: Virus-like particles and single-round infectious particles as promising alternatives
Esmeralda Cuevas-Juárez, Victoria Pando-Robles, Laura A. Palomares
Vaccine.2021; 39(48): 6990. CrossRef - NS1 Recombinant Proteins Are Efficiently Produced in Pichia pastoris and Have Great Potential for Use in Diagnostic Kits for Dengue Virus Infections
Mariana Fonseca Xisto, John Willians Oliveira Prates, Ingrid Marques Dias, Roberto Sousa Dias, Cynthia Canedo da Silva, Sérgio Oliveira de Paula
Diagnostics.2020; 10(6): 379. CrossRef - Tick-Borne Encephalitis Virus: A Quest for Better Vaccines against a Virus on the Rise
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Vaccines.2020; 8(3): 451. CrossRef - Virus-Like Particle Systems for Vaccine Development against Viruses in the Flaviviridae Family
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Vaccines.2019; 7(4): 123. CrossRef - ON MODERN APPROACHES TO CREATION OF A SINGLE-CYCLE VACCINE AGAINST TICK-BORNE ENCEPHALITIS
V. A. Lashkevich, G. G. Karganova
Problems of Virology.2018; 63(3): 101. CrossRef - Production of an enzymatically active and immunogenic form of ectodomain of Porcine rubulavirus hemagglutinin-neuraminidase in the yeast Pichia pastoris
José Luis Cerriteño-Sánchez, Gerardo Santos-López, Nora Hilda Rosas-Murrieta, Julio Reyes-Leyva, Sandra Cuevas-Romero, Irma Herrera-Camacho
Journal of Biotechnology.2016; 223: 52. CrossRef
- Influence of adjuvant type and route of administration on the immunogenicity of Leishmania-derived tick-borne encephalitis virus-like particles – A recombinant vaccine candidate
Article
- Prevalence of Tick-Borne Encephalitis Virus in Ixodid Ticks Collected from the Republic of Korea During 2011–2012
- Seok-Min Yun, Bong Gu Song, WooYoung Choi, Won Il Park, Sung Yun Kim, Jong Yul Roh, Jungsang Ryou, Young Ran Ju, Chan Park, E-Hyun Shin
- Osong Public Health Res Perspect. 2012;3(4):213-221. Published online December 31, 2012
- DOI: https://doi.org/10.1016/j.phrp.2012.10.004
- 4,025 View
- 29 Download
- 30 Crossref
-
Abstract
PDF
- Objectives
In this study, we demonstrated that TBEV-infected ticks have been distributed in the ROK, combined with our previous results. These results suggest that TBEV may exist in the ROK, and H. longicornis, H. flava, and I. nipponensis may be potential vectors of TBEV. In addition, these results emphasize the need for further epidemiological research of TBEV.
Methods
We examined for the presence of RNA of TBEV by reverse transcriptase-nested polymerase chain reaction (RT-nested PCR) using ixodid ticks captured in 25 localities of 10 provinces. Ticks were collected by the flagging and dragging method or using sentinel BG traps at forests, grass thickets, and grassland. A total of 13,053 ticks belonging to two genera and four species were collected and pooled (1292 pools), according to collection site, species of tick, and developmental stage.
Results
Among 1292 pools, the envelope (E) protein gene of TBEV was detected using RT-nested PCR in 10 pools (3 pools of the 1,331 adult ticks and 7 pools of the 11,169 nymph ticks) collected from Gangwon-do province, Jeonrabuk-do province, and Jeju Island. The minimum infection rates for TBEV of Haemaphysalis longicornis, Haemaphysalis flava, and Ixodes nipponensis were 0.06%, 0.17%, and 2.38%, respectively. Phylogenetic analysis based on the partial E protein gene was performed to identify relationships between the TBEV strains. This showed that 10 Korean strains clustered with the Western subtype.
Conclusion
In this study, we investigated the prevalence of tick-borne encephalitis virus (TBEV) in ixodid ticks from various regions of the Republic of Korea (ROK) during 2011–2012 to identify whether TBEV is circulating and to determine the endemic regions of TBEV. -
Citations
Citations to this article as recorded by- Xinyang flavivirus, from Haemaphysalis flava ticks in Henan Province, China, defines a basal, likely tick-only Orthoflavivirus clade
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