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Profiling Virulence and Antimicrobial Resistance Markers of Enterovirulent Escherichia Coli from Fecal Isolates of Adult Patients with Enteric Infections in West Cameroon
Wiliane J. T. Marbou, Priyanka Jain, Sriparna Samajpati, Gourab Halder, Asish K. Mukhopadhyay, Shanta Dutta, Victor Kuete
Osong Public Health Res Perspect. 2020;11(4):216-230.   Published online August 31, 2020
DOI: https://doi.org/10.24171/j.phrp.2020.11.4.11
  • 5,102 View
  • 99 Download
  • 4 Citations
AbstractAbstract PDF
Objectives

This study aimed to identify virulent and antimicrobial resistant genes in fecal E. coli in Mbouda, Cameroon.

Methods

A total of 599 fecal samples were collected from patients with enteric infections who were ≥ 20 years old. E. coli was isolated on the MacConkey agar and virulent genes were detected by multiplex/simplex PCR. Isolates in which ≥ 1 virulent gene was detected were subjected to antibiotic susceptibility testing. The resulting resistant isolates were subjected to PCR, followed by sequencing for resistant genes detection.

Results

There were 119 enterovirulent E. coli identified, amongst which 47.05% were atypical enteropathogenic E. coli (EPEC), 36.97% enterotoxigenic E. coli, 10.08% Shiga toxin producing E. coli (STEC) and 5.88% were enteroinvasive E. coli (EIEC). The occurrence of the eae gene (47.06%) was higher compared with CVD432 (33.61%), aaic (13.45%), stx2 (10.08%) and stx1 (0.84%). High resistance rates were noted for ampicillin (94.64% EPEC, 91.67% STEC, 59.09% EAEC, and 57.14% EIEC) and sulfamethoxazole-trimethoprim (100% EPEC and 83.33% STEC, 81.82% EAEC and 71.43% EIEC). sul2 (71.43%), tetB (64.71%), tetA (59.94%) and blaTEM (52.10%) were detected. A double mutation (S83L; D87N) was seen in gyrA and a single mutation (S80I) was observed in parC.

Conclusion

These findings suggested that measures should be taken to reduce the harm of E. coli to public health.

Citations

Citations to this article as recorded by  
  • Methanol extract from the seeds of Persea americana displays antibacterial and wound healing activities in rat model
    Steve E. Ekom, Jean-De-Dieu Tamokou, Victor Kuete
    Journal of Ethnopharmacology.2022; 282: 114573.     CrossRef
  • Characterization of diarrhoeagenic Escherichia coli with special reference to antimicrobial resistance isolated from hospitalized diarrhoeal patients in Kolkata (2012–2019), India
    Debjani Ghosh, Goutam Chowdhury, Prosenjit Samanta, Sreeja Shaw, Alok K. Deb, Mainak Bardhan, Asis Manna, Shin-ichi Miyoshi, Thandavarayan Ramamurthy, Shanta Dutta, Asish K. Mukhopadhyay
    Journal of Applied Microbiology.2022; 132(6): 4544.     CrossRef
  • Antibiotic resistomes and their chemical residues in aquatic environments in Africa
    Aemere Ogunlaja, Olumuyiwa O. Ogunlaja, Olumide D. Olukanni, Gloria O. Taylor, Chidinma G. Olorunnisola, Victorien T. Dougnon, Wassiyath Mousse, Despo Fatta-Kassinos, Titus A.M. Msagati, Emmanuel I. Unuabonah
    Environmental Pollution.2022; 312: 119783.     CrossRef
  • Antibacterial and Therapeutic Potentials of the Capsicum annuum Extract against Infected Wound in a Rat Model with Its Mechanisms of Antibacterial Action
    Steve Endeguele Ekom, Jean-De-Dieu Tamokou, Victor Kuete, Dorota Formanowicz
    BioMed Research International.2021; 2021: 1.     CrossRef
Association between Beta-lactam Antibiotic Resistance and Virulence Factors in AmpC Producing Clinical Strains of P. aeruginosa
Sanaz Dehbashi, Hamed Tahmasebi, Mohammad Reza Arabestani
Osong Public Health Res Perspect. 2018;9(6):325-333.   Published online December 31, 2018
DOI: https://doi.org/10.24171/j.phrp.2018.9.6.06
  • 21,549 View
  • 130 Download
  • 12 Citations
AbstractAbstract PDF
Objectives

The purpose of this study was to determine the presence of IMP and OXA genes in clinical strains of Pseudomonas aeruginosa (P. aeruginosa) that are carriers of the ampC gene.

Methods

In this study, 105 clinical isolates of P. aeruginosa were collected. Antibiotic resistance patterns were determined using the disk diffusion method. The strains carrying AmpC enzymes were characterized by a combination disk method. Multiplex-PCR was used to identify resistance and virulence genes, chi-square test was used to determine the relationship between variables.

Results

Among 105 isolates of P. aeruginosa, the highest antibiotic resistance was to cefotaxime and aztreonam, and the least resistance was to colictin and ceftazidime. There were 49 isolates (46.66%) that showed an AmpC phenotype. In addition, the frequencies of the resistance genes were; OXA48 gene 85.2%, OXA199, 139 3.8%, OXA23 3.8%, OXA2 66.6%, OXA10 3.8%, OXA51 85.2% and OXA58 3.8%. The IMP27 gene was detected in 9 isolates (8.57%) and the IMP3.34 was detected in 11 isolates (10.47%). Other genes detected included; lasR (17.1%), lasB (18%) and lasA (26.6%). There was a significant relationship between virulence factors and the OX and IMP genes (p ≤ 0.05).

Conclusion

The relationship between antibiotic resistance and virulence factors observed in this study could play an important role in outbreaks associated with P. aeruginosa infections.

Citations

Citations to this article as recorded by  
  • Molecular epidemiology and collaboration of siderophore-based iron acquisition with surface adhesion in hypervirulent Pseudomonas aeruginosa isolates from wound infections
    Hamed Tahmasebi, Sanaz Dehbashi, Mona Nasaj, Mohammad Reza Arabestani
    Scientific Reports.2022;[Epub]     CrossRef
  • Decoding Genetic Features and Antimicrobial Susceptibility of Pseudomonas aeruginosa Strains Isolated from Bloodstream Infections
    Tomasz Bogiel, Dagmara Depka, Mateusz Rzepka, Agnieszka Mikucka
    International Journal of Molecular Sciences.2022; 23(16): 9208.     CrossRef
  • Prevalence of the Genes Associated with Biofilm and Toxins Synthesis amongst the Pseudomonas aeruginosa Clinical Strains
    Tomasz Bogiel, Dagmara Depka, Mateusz Rzepka, Joanna Kwiecińska-Piróg, Eugenia Gospodarek-Komkowska
    Antibiotics.2021; 10(3): 241.     CrossRef
  • A Comprehensive Study of the Relationship between the Production of β-Lactamase Enzymes and Iron/Siderophore Uptake Regulatory Genes in Clinical Isolates of Acinetobacter baumannii
    Mahyar Porbaran, Hamed Tahmasebi, MohammadReza Arabestani, Joseph Falkinham
    International Journal of Microbiology.2021; 2021: 1.     CrossRef
  • Regulation of virulence and β-lactamase gene expression in Staphylococcus aureus isolates: cooperation of two-component systems in bloodstream superbugs
    Sanaz Dehbashi, Hamed Tahmasebi, Behrouz Zeyni, Mohammad Reza Arabestani
    BMC Microbiology.2021;[Epub]     CrossRef
  • New approach to identify colistin‐resistant Pseudomonas aeruginosa by high‐resolution melting curve analysis assay
    H. Tahmasebi, S. Dehbashi, M.R. Arabestani
    Letters in Applied Microbiology.2020; 70(4): 290.     CrossRef
  • Resistant Pseudomonas aeruginosa carrying virulence genes in hospitalized patients with urinary tract infection from Sanandaj, west of Iran
    Safoura Derakhshan, Aslan Hosseinzadeh
    Gene Reports.2020; 20: 100675.     CrossRef
  • Prevalence and molecular typing of Metallo-β-lactamase-producing Pseudomonas aeruginosa with adhesion factors: A descriptive analysis of burn wounds isolates from Iran
    Hamed Tahmasebi, Sanaz Dehbashi, Mohammad Yousef Alikhani, Mahyar Porbaran, Mohammad Reza Arabestani
    Gene Reports.2020; 21: 100853.     CrossRef
  • Co-harboring of mcr-1 and β-lactamase genes in Pseudomonas aeruginosa by high-resolution melting curve analysis (HRMA): Molecular typing of superbug strains in bloodstream infections (BSI)
    Hamed Tahmasebi, Sanaz Dehbashi, Mohammad Reza Arabestani
    Infection, Genetics and Evolution.2020; 85: 104518.     CrossRef
  • Relationship between Biofilm Regulating Operons and Various Β-Lactamase Enzymes: Analysis of the Clinical Features of Infections caused by Non-Fermentative Gram-Negative Bacilli (Nfgnb) from Iran
    Mahyar Porbaran, Reza Habibipour
    Journal of Pure and Applied Microbiology.2020; 14(3): 1723.     CrossRef
  • Carbapenem-Resistant Pseudomonas aeruginosa Strains-Distribution of the Essential Enzymatic Virulence Factors Genes
    Tomasz Bogiel, Małgorzata Prażyńska, Joanna Kwiecińska-Piróg, Agnieszka Mikucka, Eugenia Gospodarek-Komkowska
    Antibiotics.2020; 10(1): 8.     CrossRef
  • Biofilm Formation and β-lactamase Enzymes: A Synergism Activity in Acinetobacter baumannii Isolated from Wound Infection
    Mahyar Porbaran, Reza Habibipour
    Journal of Advances in Medical and Biomedical Rese.2019; 27(125): 34.     CrossRef
Relationships between Virulence Factors and Antimicrobial Resistance among Escherichia coli Isolated from Urinary Tract Infections and Commensal Isolates in Tehran, Iran
Mohammad Reza Asadi Karam, Mehri Habibi, Saeid Bouzari
Osong Public Health Res Perspect. 2018;9(5):217-224.   Published online October 31, 2018
DOI: https://doi.org/10.24171/j.phrp.2018.9.5.02
  • 4,703 View
  • 110 Download
  • 20 Citations
AbstractAbstract PDF
Objectives

Uropathogenic Escherichia coli (UPEC) are the major cause of urinary tract infections (UTIs). Here, we determined whether sensitivity to antibiotics was related to the prevalence of iron scavenging genes, or to biofilm and hemolysis formation.

Methods

A total of 110 UPEC and 30 E coli isolates were collected from the urine of UTI patients and feces of healthy individuals without UTI, respectively. The presence of iron receptor genes and phenotypic properties were evaluated by polymerase chain reaction and phenotypic methods, respectively. Susceptibility to routine antibiotics was evaluated using the disc diffusion method.

Results

The prevalence of iron scavenging genes ranged from 21.8% (ireA) to 84.5% (chuA) in the UPEC. Resistance to ceftazidime and cefotaxime was significantly correlated with the presence of fyuA and iutA iron genes. Biofilm production was significantly associated with the prevalence of fyuA and hma iron genes. A higher degree of antibiotic resistance was exhibited by isolates that produced biofilms than by their non-biofilm producing counterparts.

Conclusion

Our study clearly indicates that biofilm production is associated with antibiotic resistance, and that iron receptors and hemolysin production also contribute to reduced antibiotic sensitivity. These results further our understanding of the role that these virulence factors play during UPEC pathogenesis, which in turn may be valuable for the development of novel treatment strategies against UTIs.

Citations

Citations to this article as recorded by  
  • Susceptibility and Virulence of Enterobacteriaceae Isolated from Urinary Tract Infections in Benin
    Funkè F. Assouma, Haziz Sina, Tomabu Adjobimey, Agossou Damien Pacôme Noumavo, Akim Socohou, Bawa Boya, Ange D. Dossou, Lauriane Akpovo, Basile Boni Saka Konmy, Jacques F. Mavoungou, Adolphe Adjanohoun, Lamine Baba-Moussa
    Microorganisms.2023; 11(1): 213.     CrossRef
  • Characterization of virulence determinants and phylogenetic background of multiple and extensively drug resistant Escherichia coli isolated from different clinical sources in Egypt
    Rana El-baz, Heba Shehta Said, Eman Salama Abdelmegeed, Rasha Barwa
    Applied Microbiology and Biotechnology.2022; 106(3): 1279.     CrossRef
  • A global systematic review and meta-analysis on correlation between biofilm producers and non-biofilm producers with antibiotic resistance in Uropathogenic Escherichia coli
    Mitra Garousi, Sina Monazami Tabar, Hosein Mirazi, Parnia Asgari, Paniz Sabeghi, Astireh Salehi, Azad Khaledi, Mohammad Ghenaat Pisheh Sanani, Hossein Karballaei Mirzahosseini
    Microbial Pathogenesis.2022; 164: 105412.     CrossRef
  • Virulence factors, antimicrobial resistance and the relationship between these characteristics in uropathogenic Escherichia coli
    Farzaneh Firoozeh, Mohammad Zibaei, Farzad Badmasti, Azad Khaledi
    Gene Reports.2022; 27: 101622.     CrossRef
  • Association between Virulence Factors and Antimicrobial Resistance of Klebsiella pneumoniae Clinical Isolates from North Kerala
    Ramya Kumaran, R.V. Geetha, Sabitha Baby
    Journal of Pure and Applied Microbiology.2022; 16(2): 867.     CrossRef
  • Characterization of virulence factors and antibiotic resistance pattern of uropathogenic Escherichia coli strains in a tertiary care center
    Naveen Kumar M, Sevitha Bhat, Archana Bhat K, Vishwas Saralaya, Shalini Shenoy Mulki
    F1000Research.2022; 11: 1163.     CrossRef
  • Antibiotic Resistance, Biofilm Formation and Sub-Inhibitory Hydrogen Peroxide Stimulation in Uropathogenic Escherichia coli
    Prabin Dawadi, Santosh Khanal, Tista Prasai Joshi, Sudeep KC, Reshma Tuladhar, Bijaya Laxmi Maharjan, Anjani Darai, Dev Raj Joshi
    Microbiology Insights.2022; 15: 117863612211352.     CrossRef
  • Characterization of virulence factors and antibiotic resistance pattern of uropathogenic Escherichia coli strains in a tertiary care center
    Naveen Kumar M, Sevitha Bhat, Archana Bhat K, Vishwas Saralaya, Shalini Shenoy Mulki
    F1000Research.2022; 11: 1163.     CrossRef
  • Insects, Rodents, and Pets as Reservoirs, Vectors, and Sentinels of Antimicrobial Resistance
    Willis Gwenzi, Nhamo Chaukura, Norah Muisa-Zikali, Charles Teta, Tendai Musvuugwa, Piotr Rzymski, Akebe Luther King Abia
    Antibiotics.2021; 10(1): 68.     CrossRef
  • Virulence genes and phylogenetic groups of uropathogenic Escherichia coli isolates from patients with urinary tract infection and uninfected control subjects: a case-control study
    Seyedeh Elham Rezatofighi, Mahsa Mirzarazi, Mansour Salehi
    BMC Infectious Diseases.2021;[Epub]     CrossRef
  • Virulence characterization and clonal analysis of uropathogenic Escherichia coli metallo-beta-lactamase-producing isolates
    Fatemeh Zangane Matin, Seyedeh Elham Rezatofighi, Mohammad Roayaei Ardakani, Mohammad Reza Akhoond, Fahimeh Mahmoodi
    Annals of Clinical Microbiology and Antimicrobials.2021;[Epub]     CrossRef
  • Antimicrobial Susceptibility and Detection of Virulence-Associated Genes in Escherichia coli Strains Isolated from Commercial Broilers
    Tímea Kocúreková, Lívia Karahutová, Dobroslava Bujňáková
    Antibiotics.2021; 10(11): 1303.     CrossRef
  • Evaluation of Biofilm Formation and Virulence Genes and Association with Antibiotic Resistance Patterns of Uropathogenic Escherichia coli Strains in Southwestern Iran
    Mostafa Boroumand, Asghar Sharifi, Mohammad Amin Ghatei, Mohsen Sadrinasab
    Jundishapur Journal of Microbiology.2021;[Epub]     CrossRef
  • Differences of virulence factors, and antimicrobial susceptibility according to phylogenetic group in uropathogenic Escherichia coli strains isolated from Korean patients
    Miri Hyun, Ji Yeon Lee, Hyun ah Kim
    Annals of Clinical Microbiology and Antimicrobials.2021;[Epub]     CrossRef
  • Changes in Bacterial Spectrum and Resistance Patterns Over Time in the Urine of Patients with Neurogenic Lower Urinary Tract Dysfunction Due to Spinal Cord Injury
    Jürgen Pannek, Carmen Kurmann, Jörg Krebs, Valentin Habermacher, Jens Wöllner
    Urologia Internationalis.2021; 105(5-6): 483.     CrossRef
  • A systematic review and meta-analysis of antibiotic resistance patterns, and the correlation between biofilm formation with virulence factors in uropathogenic E. coli isolated from urinary tract infections
    Fei Zhao, Huanxin Yang, Dezhong Bi, Azad Khaledi, Mingqi Qiao
    Microbial Pathogenesis.2020; 144: 104196.     CrossRef
  • A survey for phylogenetic relationship; presence of virulence genes and antibiotic resistance patterns of avian pathogenic and uropathogenic Escherichia coli isolated from poultry and humans in Yazd, Iran
    Mansoureh Bakhshi, Hengameh Zandi, Mehdi Fatahi Bafghi, Akram Astani, Vahid Reza Ranjbar, Mahmood Vakili
    Gene Reports.2020; 20: 100725.     CrossRef
  • Biofilm formation, antimicrobial susceptibility and virulence genes of Uropathogenic Escherichia coli isolated from clinical isolates in Uganda
    Paul Katongole, Fatuma Nalubega, Najjuka Christine Florence, Benon Asiimwe, Irene Andia
    BMC Infectious Diseases.2020;[Epub]     CrossRef
  • Relationship between Virulence and Resistance among Gram-Negative Bacteria
    Virginio Cepas, Sara M. Soto
    Antibiotics.2020; 9(10): 719.     CrossRef
  • Virulence factors of uropathogenic Escherichia coli (UPEC) and correlation with antimicrobial resistance
    Chhaya Shah, Ratna Baral, Bijay Bartaula, Lok Bahadur Shrestha
    BMC Microbiology.2019;[Epub]     CrossRef
Profiling of Virulence-associated Factors in Shigella Species Isolated from Acute Pediatric Diarrheal Samples in Tehran, Iran
Sajad Yaghoubi, Reza Ranjbar, Mohammad Mehdi Soltan Dallal, Somayeh Yasliani Fard, Mohammad Hasan Shirazi, Mahmood Mahmoudi
Osong Public Health Res Perspect. 2017;8(3):220-226.   Published online June 30, 2017
DOI: https://doi.org/10.24171/j.phrp.2017.8.3.09
  • 3,174 View
  • 56 Download
  • 15 Citations
AbstractAbstract PDF
Objectives

The genus Shigella comprises the most infectious and diarrheagenic bacteria causing severe diseases, mostly in children under five years of age. This study aimed to detect nine virulence genes (ipaBCD, VirA, sen, set1A, set1B, ial, ipaH, stx, and sat) in Shigella species (spp.) using multiplex polymerase chain reaction (MPCR) and to determine the relation of Shigella spp. from pediatric diarrheal samples with hospitalization and bloody diarrhea in Tehran, Iran.

Methods

Shigella spp. were isolated and identified using standard microbiological and serological methods. The virulence genes were detected using MPCR.

Results

Seventy-five Shigella spp. (40 S. sonnei, 33 S. flexneri, 1 S. dysenteriae, and 1 S. boydii) were isolated in this study. The prevalence of ial, sen, sat, set1A, and set1B was 74.7%, 45.4%, 28%, 24%, and 24%, respectively. All S. flexneri isolates, while no S. sonnei, S. dysenteriae, or S. boydii isolates, contained sat, set1A, and set1B. All isolates were positive for ipaH, ipaBCD, and virA, while one (1.4%) of the isolates contained stx. The highest prevalence of virulence determinants was found in S. flexneri serotype IIa. Nineteen (57.6%) of 33 S. flexneri isolates were positive for ipaBCD, ipaH, virA, ial, and sat. The sen determinants were found to be statistically significantly associated with hospitalization and bloody diarrhea (p = 0.001).

Conclusion

This study revealed a high prevalence of enterotoxin genes in S. flexneri, especially in serotype 2a, and has presented relations between a few clinical features of shigellosis and numerous virulence determinants of clinical isolates of Shigella spp.

Citations

Citations to this article as recorded by  
  • Plant-derived nanoparticles as alternative therapy against Diarrheal pathogens in the era of antimicrobial resistance: A review
    Tesleem Olatunde Abolarinwa, Daniel Jesuwenu Ajose, Bukola Opeyemi Oluwarinde, Justine Fri, Kotsoana Peter Montso, Omolola Esther Fayemi, Adeyemi Oladapo Aremu, Collins Njie Ateba
    Frontiers in Microbiology.2022;[Epub]     CrossRef
  • Distribution of genes encoding virulence factors of Shigella strains isolated from children with diarrhea in southwest Iran
    Nabi Jomehzadeh, Khadijah Ahmadi, Hazhir Javaherizadeh, Maryam Afzali
    Molecular Biology Reports.2021; 48(2): 1645.     CrossRef
  • Evaluate the distribution of virulence genes and to investigate antibiotic resistance pattern among Shigella species isolated from children with shigellosis in Iran
    Samane Mohebi, Hossein Hosseini Nave, Kasra Javadi, Ali Amanati, Soudeh Kholdi, Mahtab Hadadi, Zahra Hashemizadeh, Mohammad Motamedifar
    Gene Reports.2021; 23: 101189.     CrossRef
  • Burden, Antibiotic Resistance, and Clonality of Shigella spp. Implicated in Community-Acquired Acute Diarrhoea in Lilongwe, Malawi
    Abel F.N.D. Phiri, Akebe Luther King Abia, Daniel Gyamfi Amoako, Rajab Mkakosya, Arnfinn Sundsfjord, Sabiha Y. Essack, Gunnar Skov Simonsen
    Tropical Medicine and Infectious Disease.2021; 6(2): 63.     CrossRef
  • Frequency and Antimicrobial Resistance of Shigella Species in Iran During 2000-2020
    Farhad Moradi, Nahal Hadi, Maryam Akbari, Zahra Hashemizadeh, Reyhaneh Rouhi Jahromi
    Jundishapur Journal of Health Sciences.2021;[Epub]     CrossRef
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    Zhen Zhu, Weiwei Wang, Mingze Cao, Qiqi Zhu, Tenghe Ma, Yongying Zhang, Guanhui Liu, Xuzheng Zhou, Bing Li, Yuxiang Shi, Jiyu Zhang
    BMC Microbiology.2021;[Epub]     CrossRef
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    B. Roy, S.K. Tousif Ahamed, B. Bandyopadhyay, N. Giri
    Letters in Applied Microbiology.2020; 71(1): 86.     CrossRef
  • High Rates of Antimicrobial Resistance and Virulence Gene Distribution Among Shigella spp. Isolated from Pediatric Patients in Tehran, Iran


    Mohammadmahdi Karimi-Yazdi, Zohreh Ghalavand, Mahdi Shabani, Hamidreza Houri, Mehrzad Sadredinamin, Marzieh Taheri, Gita Eslami
    Infection and Drug Resistance.2020; Volume 13: 485.     CrossRef
  • Molecular characterization of Shigella species isolated from diarrheal patients in Tehran, Iran: phylogenetic typing and its association with virulence gene profiles and a novel description of Shigella invasion associated locus
    Sina Arabshahi, Aytak Novinrooz, Reza Ranjbar, Abbas Ali Imani Fooladi
    European Journal of Clinical Microbiology & Infect.2020; 39(9): 1727.     CrossRef
  • Case report on a swift shift in uropathogens from Shigella flexneri to Escherichia coli: a thin line between bacterial persistence and reinfection
    Kukwah Anthony Tufon, Djike Puepi Yolande Fokam, Youmbi Sylvain Kouanou, Henry Dilonga Meriki
    Annals of Clinical Microbiology and Antimicrobials.2020;[Epub]     CrossRef
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    Mariana Bona, Pedro Henrique Medeiros, Ana Karolina Santos, Thiago Freitas, Mara Prata, Herlice Veras, Marília Amaral, Daniel Oliveira, Alexandre Havt, Aldo Ângelo Lima
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    Hamed Memariani, Mojtaba Memariani
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    Dhiviya Prabaa Muthuirulandi Sethuvel, Shalini Anandan, Joy Sarojini Michael, Dhivya Murugan, Ayyanraj Neeravi, Valsan Philip Verghese, Kamini Walia, Balaji Veeraraghavan
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    Mojtaba Moosavian, Sakineh Seyed-Mohammadi, Ahmad Farajzadeh Sheikh, Saeed Khoshnood, Aram Asarehzadegan Dezfuli, Morteza Saki, Gholamreza Ghaderian, Fatemeh Shahi, Mahtab Abdi, Fariba Abbasi
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  • Frequency of Mutations in Quinolone Resistance-Determining Regions and Plasmid-Mediated Quinolone Resistance in Shigella Isolates Recovered from Pediatric Patients in Tehran, Iran: An Overlooked Problem
    Sajad Yaghoubi, Reza Ranjbar, Mohammad Mehdi Soltan Dallal, Mohammad Hasan Shirazi, Mohammad Kazem Sharifi-Yazdi
    Microbial Drug Resistance.2018; 24(6): 699.     CrossRef
Phenotypic Assays to Determine Virulence Factors of Uropathogenic Escherichia coli (UPEC) Isolates and their Correlation with Antibiotic Resistance Pattern
Mohsen Tabasi, Mohammad Reza Asadi Karam, Mehri Habibi, Mir Saeed Yekaninejad, Saeid Bouzari
Osong Public Health Res Perspect. 2015;6(4):261-268.   Published online August 31, 2015
DOI: https://doi.org/10.1016/j.phrp.2015.08.002
  • 2,423 View
  • 18 Download
  • 39 Citations
AbstractAbstract PDF
Objectives
Urinary tract infection caused by uropathogenic Escherichia coli (UPEC) strains is one of the most important infections in the world. UPEC encode widespread virulence factors closely related with pathogenesis of the bacteria. The purpose of this study was to evaluate the presence of different phenotypic virulence markers in UPEC isolates and determine their correlation with antibiotic resistance pattern.
Methods
UPEC isolates from patients with different clinical symptoms of UTI were collected and screened for biofilm and hemolysin production, mannose resistant, and mannose sensitive hemagglutination (MRHA and MSHA, respectively). In addition, antimicrobial resistance pattern and ESBL-producing isolates were recorded.
Results
Of the 156 UPEC isolates, biofilm and hemolysin formation was seen in 133 (85.3%) and 53 (34%) isolates, respectively. Moreover, 98 (62.8%) and 58 (37.2%) isolates showed the presence of Types 1 fimbriae (MSHA) and P fimbriae (MRHA), respectively. Our results also showed a relationship between biofilm formation in UPEC isolated from acute cystitis patients and recurrent UTI cases. Occurrence of UTI was dramatically correlated with the patients' profiles. We observed that the difference in antimicrobial susceptibilities of the biofilm and nonbiofilm former isolates was statistically significant. The UPEC isolates showed the highest resistance to ampicillin, tetracycline, amoxicillin, and cotrimoxazole. Moreover, 26.9% of isolates were ESBL producers.
Conclusion
This study indicated that there is a relationship between the phenotypic virulence traits of the UPEC isolates, patients' profiles, and antibiotic resistance. Detection of the phenotypic virulence factors could help to improve understanding of pathogenesis of UPEC isolates and better medical intervention.

Citations

Citations to this article as recorded by  
  • Detection of Adhesion Encoding Genes, Antibacterial Susceptibility Test and Biofilm Formation of Uropathogenic Escherichia coli Isolated from Urinary Tract Infections in Children
    Rezvan Goodarzi, Rasoul Yousefimashouf, Iraj Sedighi, Abbas Moradi, Fatemeh Nouri, Mohammad Taheri
    Journal of Advances in Medical and Biomedical Rese.2022; 30(1): 1.     CrossRef
  • A global systematic review and meta-analysis on correlation between biofilm producers and non-biofilm producers with antibiotic resistance in Uropathogenic Escherichia coli
    Mitra Garousi, Sina Monazami Tabar, Hosein Mirazi, Parnia Asgari, Paniz Sabeghi, Astireh Salehi, Azad Khaledi, Mohammad Ghenaat Pisheh Sanani, Hossein Karballaei Mirzahosseini
    Microbial Pathogenesis.2022; 164: 105412.     CrossRef
  • Antibiotic resistance, phylogenetic typing, and virulence genes profile analysis of uropathogenic Escherichia coli isolated from patients in southern Iraq
    Mohammed Allami, Masoumeh Bahreini, Mohammad Reza Sharifmoghadam
    Journal of Applied Genetics.2022; 63(2): 401.     CrossRef
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    Mehrdad Halaji, Amirhossein Fayyazi, Mehdi Rajabnia, Donya Zare, Abazar Pournajaf, Reza Ranjbar
    Frontiers in Cellular and Infection Microbiology.2022;[Epub]     CrossRef
  • Genotypic assay to determine some virulence factors of Uropathogenic E. coli (UPEC) isolates
    Tsahel H. Al-Dulaimi, Ilham A Bunyan, Thikra A. Banimuslem
    International journal of health sciences.2022; : 1593.     CrossRef
  • Demonstrating the utility of Escherichia coli asymptomatic bacteriuria isolates’ virulence profile towards diagnosis and management—A preliminary analysis
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Multilocus Sequence Analysis of Housekeeping Genes and Antigenic Determinant Genes in Bordetella pertussis Strains Isolated in Korea
Sang-Oun Jung, Yu Mi Moon, So-Hyeon Kim, Hwa Young Sung, Seung-Jik Kwon, Yeon Ho Kang, Jae Yon Yu
Osong Public Health Res Perspect. 2011;2(2):115-126.   Published online June 30, 2011
DOI: https://doi.org/10.1016/j.phrp.2011.08.003
  • 2,164 View
  • 13 Download
  • 3 Citations
AbstractAbstract PDF
Objectives
To confirm genotype diversities of clinical isolates of Bordetella pertussis and to evaluate the risk of pertussis outbreak in Korea.
Methods
Seven housekeeping genes and 10 antigenic determinant genes from clinical B. pertussis isolates were analyzed by Multilocus sequence typing (MLST).
Results
More variant pattern was observed in antigenic determinant genes. Especially, PtxS1 gene was the most variant gene; five genotypes were observed from eight global genotypes. In the bacterial type, the number of observed sequence types in the isolates was seven and the most frequent form was type 1 (79.6%). This major sequence type also showed a time-dependent transition pattern. Older isolates (1968 and 1975) showed type 1 and 6 in housekeeping genes and antigenic determinant genes, respectively. However, these were changed to type 2 and 1 in isolates 1999–2008. This transition was mainly attributed to genotype change of PtxS1 and Fim3 gene; the tendency of genotype change was to avoid vaccine-derived genotype. In addition, there was second transition in 2009. In this period, only the sequence type of antigenic determinant genes was changed to type 2. Based Upon Related Sequence Types (BURST) analysis confirmed that there were two clonal complexes (ACCI and ACCII) in the Korean isolates. Moreover, the recently increased sequence type was revealed as AST2 derived from AST 3 in ACCI.
Conclusions
Genotype changes in Korean distributing strains are still progressing and there was a specific driving force in antigenic determinant genes. Therefore continuous surveillance of genotype change of the distributing strains should be performed to confirm interrelationship of genotype change with vaccine immunity.

Citations

Citations to this article as recorded by  
  • Characterization of Bordetella pertussis Strains Isolated from India
    Shweta Alai, Manish Gautam, Sonali Palkar, Jitendra Oswal, Sunil Gairola, Dhiraj P. Dhotre
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Distribution of Virulence Genes and Their Association of Serotypes in Pathogenic Escherichia coli Isolates From Diarrheal Patients in Korea
Seung-Hak Cho, Kyung-Hwan Oh, Seong-Han Kim, Hee-Bok Oh, Mi-Sun Park
Osong Public Health Res Perspect. 2010;1(1):29-35.   Published online December 31, 2010
DOI: https://doi.org/10.1016/j.phrp.2010.12.008
  • 2,252 View
  • 15 Download
  • 6 Citations
AbstractAbstract PDF
Objectives
To characterise the genetic and serological diversity of pathogenic Escherichia coli, we tested 111 E coli strains isolated from diarrhoeal patients in Korea between 2003 and 2006.
Methods
The isolates were tested through polymerase chain reaction (PCR) and slide agglutination method for the detection of virulence genes and serotypes, respectively. To compare the expression of Shiga toxin (stx)-1 and stx2 genes, real-time quantitative reverse-transcriptase PCR and rapid exprssion assay, reversed-passive latex agglutination, were performed.
Results
Forty-nine Shiga toxin-producing E coli (STEC) strains and 62 non-STEC strains, including 20 enteropathogenic E coli, 20 enterotoxigenic E coli, 20 enteroaggregative E coli, and 2 enteroinvasive E coli were randomly chosen from the strains isolated from diarrhoeal patients in Korea between 2003 and 2006. PCR analysis indicated that locus of enterocyte effacement pathogenicity island, that is, eaeA, espADB, and tir genes were present in STEC, enteropathogenic E coli, and enteroinvasive E coli. Quorum sensing-related gene luxS was detected in most of pathogenic E coli strains. Major serotypes of the STEC strains were O157 (26%) and O26 (20%), whereas the non-STEC strains possessed various serotypes. Especially, all the strains with serotype O157 carried stx2 and the tested virulence factors. Of the STEC strains, the data of real-time quantitative reverse-transcriptase PCR and reversed-passive latex agglutination tests showed that messenger RNA- and protein expression of stx2 gene were higher than those of stx1 gene.
Conclusion
Our results provide the epidemiological information regarding the trend of STEC and non-STEC infections in the general population and show the fundamental data in association of serotypes with virulence genes in diarrhoeagenic E coli strains from Korea.

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PHRP : Osong Public Health and Research Perspectives