Osong Public Health and Research Perspectives

Original Articles

Association between Beta-lactam Antibiotic Resistance and Virulence Factors in AmpC Producing Clinical Strains of P. aeruginosa: Sanaz Dehbashi, Hamed Tahmasebi, Mohammad Reza Arabestani; Osong Public Health Res Perspect. 2018;9(6):325-333. Published online December 31, 2018; DOI: https://doi.org/10.24171/j.phrp.2018.9.6.06

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Objectives

The purpose of this study was to determine the presence of IMP and OXA genes in clinical strains of Pseudomonas aeruginosa (P. aeruginosa) that are carriers of the ampC gene.

Methods

In this study, 105 clinical isolates of P. aeruginosa were collected. Antibiotic resistance patterns were determined using the disk diffusion method. The strains carrying AmpC enzymes were characterized by a combination disk method. Multiplex-PCR was used to identify resistance and virulence genes, chi-square test was used to determine the relationship between variables.

Results

Among 105 isolates of P. aeruginosa, the highest antibiotic resistance was to cefotaxime and aztreonam, and the least resistance was to colictin and ceftazidime. There were 49 isolates (46.66%) that showed an AmpC phenotype. In addition, the frequencies of the resistance genes were; OXA48 gene 85.2%, OXA199, 139 3.8%, OXA23 3.8%, OXA2 66.6%, OXA10 3.8%, OXA51 85.2% and OXA58 3.8%. The IMP27 gene was detected in 9 isolates (8.57%) and the IMP3.34 was detected in 11 isolates (10.47%). Other genes detected included; lasR (17.1%), lasB (18%) and lasA (26.6%). There was a significant relationship between virulence factors and the OX and IMP genes (p ≤ 0.05).

Conclusion

The relationship between antibiotic resistance and virulence factors observed in this study could play an important role in outbreaks associated with P. aeruginosa infections.

Citations

Citations to this article as recorded by

Comparative bacteriome and antibiotic resistome analysis of water and sediment of the Ganga River of India
Ankita Srivastava, Digvijay Verma
World Journal of Microbiology and Biotechnology.2023;[Epub] CrossRef
Prevalence of different virulence factors and their association with antimicrobial resistance among Pseudomonas aeruginosa clinical isolates from Egypt
Eva A. Edward, Marwa R. El Shehawy, Alaa Abouelfetouh, Elsayed Aboulmagd
BMC Microbiology.2023;[Epub] CrossRef
Molecular epidemiology and collaboration of siderophore-based iron acquisition with surface adhesion in hypervirulent Pseudomonas aeruginosa isolates from wound infections
Hamed Tahmasebi, Sanaz Dehbashi, Mona Nasaj, Mohammad Reza Arabestani
Scientific Reports.2022;[Epub] CrossRef
Decoding Genetic Features and Antimicrobial Susceptibility of Pseudomonas aeruginosa Strains Isolated from Bloodstream Infections
Tomasz Bogiel, Dagmara Depka, Mateusz Rzepka, Agnieszka Mikucka
International Journal of Molecular Sciences.2022; 23(16): 9208. CrossRef
Prevalence of the Genes Associated with Biofilm and Toxins Synthesis amongst the Pseudomonas aeruginosa Clinical Strains
Tomasz Bogiel, Dagmara Depka, Mateusz Rzepka, Joanna Kwiecińska-Piróg, Eugenia Gospodarek-Komkowska
Antibiotics.2021; 10(3): 241. CrossRef
A Comprehensive Study of the Relationship between the Production of β-Lactamase Enzymes and Iron/Siderophore Uptake Regulatory Genes in Clinical Isolates of Acinetobacter baumannii
Mahyar Porbaran, Hamed Tahmasebi, MohammadReza Arabestani, Joseph Falkinham
International Journal of Microbiology.2021; 2021: 1. CrossRef
Regulation of virulence and β-lactamase gene expression in Staphylococcus aureus isolates: cooperation of two-component systems in bloodstream superbugs
Sanaz Dehbashi, Hamed Tahmasebi, Behrouz Zeyni, Mohammad Reza Arabestani
BMC Microbiology.2021;[Epub] CrossRef
New approach to identify colistin‐resistant Pseudomonas aeruginosa by high‐resolution melting curve analysis assay
H. Tahmasebi, S. Dehbashi, M.R. Arabestani
Letters in Applied Microbiology.2020; 70(4): 290. CrossRef
Resistant Pseudomonas aeruginosa carrying virulence genes in hospitalized patients with urinary tract infection from Sanandaj, west of Iran
Safoura Derakhshan, Aslan Hosseinzadeh
Gene Reports.2020; 20: 100675. CrossRef
Prevalence and molecular typing of Metallo-β-lactamase-producing Pseudomonas aeruginosa with adhesion factors: A descriptive analysis of burn wounds isolates from Iran
Hamed Tahmasebi, Sanaz Dehbashi, Mohammad Yousef Alikhani, Mahyar Porbaran, Mohammad Reza Arabestani
Gene Reports.2020; 21: 100853. CrossRef
Co-harboring of mcr-1 and β-lactamase genes in Pseudomonas aeruginosa by high-resolution melting curve analysis (HRMA): Molecular typing of superbug strains in bloodstream infections (BSI)
Hamed Tahmasebi, Sanaz Dehbashi, Mohammad Reza Arabestani
Infection, Genetics and Evolution.2020; 85: 104518. CrossRef
Relationship between Biofilm Regulating Operons and Various Β-Lactamase Enzymes: Analysis of the Clinical Features of Infections caused by Non-Fermentative Gram-Negative Bacilli (Nfgnb) from Iran
Mahyar Porbaran, Reza Habibipour
Journal of Pure and Applied Microbiology.2020; 14(3): 1723. CrossRef
Carbapenem-Resistant Pseudomonas aeruginosa Strains-Distribution of the Essential Enzymatic Virulence Factors Genes
Tomasz Bogiel, Małgorzata Prażyńska, Joanna Kwiecińska-Piróg, Agnieszka Mikucka, Eugenia Gospodarek-Komkowska
Antibiotics.2020; 10(1): 8. CrossRef
Biofilm Formation and β-lactamase Enzymes: A Synergism Activity in Acinetobacter baumannii Isolated from Wound Infection
Mahyar Porbaran, Reza Habibipour
Journal of Advances in Medical and Biomedical Rese.2019; 27(125): 34. CrossRef

Relationships between Virulence Factors and Antimicrobial Resistance among Escherichia coli Isolated from Urinary Tract Infections and Commensal Isolates in Tehran, Iran: Mohammad Reza Asadi Karam, Mehri Habibi, Saeid Bouzari; Osong Public Health Res Perspect. 2018;9(5):217-224. Published online October 31, 2018; DOI: https://doi.org/10.24171/j.phrp.2018.9.5.02

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118 Download
26 Citations

Abstract PDF

Objectives

Uropathogenic Escherichia coli (UPEC) are the major cause of urinary tract infections (UTIs). Here, we determined whether sensitivity to antibiotics was related to the prevalence of iron scavenging genes, or to biofilm and hemolysis formation.

Methods

A total of 110 UPEC and 30 E coli isolates were collected from the urine of UTI patients and feces of healthy individuals without UTI, respectively. The presence of iron receptor genes and phenotypic properties were evaluated by polymerase chain reaction and phenotypic methods, respectively. Susceptibility to routine antibiotics was evaluated using the disc diffusion method.

Results

The prevalence of iron scavenging genes ranged from 21.8% (ireA) to 84.5% (chuA) in the UPEC. Resistance to ceftazidime and cefotaxime was significantly correlated with the presence of fyuA and iutA iron genes. Biofilm production was significantly associated with the prevalence of fyuA and hma iron genes. A higher degree of antibiotic resistance was exhibited by isolates that produced biofilms than by their non-biofilm producing counterparts.

Conclusion

Our study clearly indicates that biofilm production is associated with antibiotic resistance, and that iron receptors and hemolysin production also contribute to reduced antibiotic sensitivity. These results further our understanding of the role that these virulence factors play during UPEC pathogenesis, which in turn may be valuable for the development of novel treatment strategies against UTIs.

Citations

Citations to this article as recorded by

Uropathogenic Escherichia coli (UPEC)-Associated Urinary Tract Infections: The Molecular Basis for Challenges to Effective Treatment
Shane Whelan, Brigid Lucey, Karen Finn
Microorganisms.2023; 11(9): 2169. CrossRef
Susceptibility and Virulence of Enterobacteriaceae Isolated from Urinary Tract Infections in Benin
Funkè F. Assouma, Haziz Sina, Tomabu Adjobimey, Agossou Damien Pacôme Noumavo, Akim Socohou, Bawa Boya, Ange D. Dossou, Lauriane Akpovo, Basile Boni Saka Konmy, Jacques F. Mavoungou, Adolphe Adjanohoun, Lamine Baba-Moussa
Microorganisms.2023; 11(1): 213. CrossRef
Association Between Uropathogenic Escherichia coli Virulence Genes and Severity of Infection and Resistance to Antibiotics
Sofía Alejandra Fonseca-Martínez, Ruth Aralí Martínez-Vega, Ana Elvira Farfán-García, Clara Isabel González Rugeles, Libeth Yajaira Criado-Guerrero
Infection and Drug Resistance.2023; Volume 16: 3707. CrossRef
Incidence of biofilms among the multidrug resistant E. coli, isolated from urinary tract infections in the Nilgiris district, South India
A. P. Cardiliya, M. J. N. Chandrasekar, M. J. Nanjan
Brazilian Journal of Microbiology.2023; 54(3): 1809. CrossRef
Correlation of biofilm formation, virulence factors, and phylogenetic groups among Escherichia coli strains causing urinary tract infection: A global systematic review and meta-analysis
HosseinKarballaei Mirzahosseini, Farhad Najmeddin, Atabak Najafi, Arezoo Ahmadi, Hamidreza Sharifnia, Azad Khaledi, Mojtaba Mojtahedzadeh
Journal of Research in Medical Sciences.2023; 28(1): 66. CrossRef
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Shiori Kitaya, Chieko Miura, Ayano Suzuki, Yoshimichi Imai, Koichi Tokuda, Hajime Kanamori
Applied Microbiology.2023; 3(3): 1046. CrossRef
Fluoroquinolone resistance determinants in carbapenem-resistant Escherichia coli isolated from urine clinical samples in Thailand
Parichart Boueroy, Peechanika Chopjitt, Rujirat Hatrongjit, Masatomo Morita, Yo Sugawara, Yukihiro Akeda, Tetsuya Iida, Shigeyuki Hamada, Anusak Kerdsin
PeerJ.2023; 11: e16401. CrossRef
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Rana El-baz, Heba Shehta Said, Eman Salama Abdelmegeed, Rasha Barwa
Applied Microbiology and Biotechnology.2022; 106(3): 1279. CrossRef
A global systematic review and meta-analysis on correlation between biofilm producers and non-biofilm producers with antibiotic resistance in Uropathogenic Escherichia coli
Mitra Garousi, Sina Monazami Tabar, Hosein Mirazi, Parnia Asgari, Paniz Sabeghi, Astireh Salehi, Azad Khaledi, Mohammad Ghenaat Pisheh Sanani, Hossein Karballaei Mirzahosseini
Microbial Pathogenesis.2022; 164: 105412. CrossRef
Virulence factors, antimicrobial resistance and the relationship between these characteristics in uropathogenic Escherichia coli
Farzaneh Firoozeh, Mohammad Zibaei, Farzad Badmasti, Azad Khaledi
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Ramya Kumaran, R.V. Geetha, Sabitha Baby
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Naveen Kumar M, Sevitha Bhat, Archana Bhat K, Vishwas Saralaya, Shalini Shenoy Mulki
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Prabin Dawadi, Santosh Khanal, Tista Prasai Joshi, Sudeep KC, Reshma Tuladhar, Bijaya Laxmi Maharjan, Anjani Darai, Dev Raj Joshi
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Naveen Kumar M, Sevitha Bhat, Archana Bhat K, Vishwas Saralaya, Shalini Shenoy Mulki
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Virulence genes and phylogenetic groups of uropathogenic Escherichia coli isolates from patients with urinary tract infection and uninfected control subjects: a case-control study
Seyedeh Elham Rezatofighi, Mahsa Mirzarazi, Mansour Salehi
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Tímea Kocúreková, Lívia Karahutová, Dobroslava Bujňáková
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Mostafa Boroumand, Asghar Sharifi, Mohammad Amin Ghatei, Mohsen Sadrinasab
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Differences of virulence factors, and antimicrobial susceptibility according to phylogenetic group in uropathogenic Escherichia coli strains isolated from Korean patients
Miri Hyun, Ji Yeon Lee, Hyun ah Kim
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Fei Zhao, Huanxin Yang, Dezhong Bi, Azad Khaledi, Mingqi Qiao
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A survey for phylogenetic relationship; presence of virulence genes and antibiotic resistance patterns of avian pathogenic and uropathogenic Escherichia coli isolated from poultry and humans in Yazd, Iran
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Biofilm formation, antimicrobial susceptibility and virulence genes of Uropathogenic Escherichia coli isolated from clinical isolates in Uganda
Paul Katongole, Fatuma Nalubega, Najjuka Christine Florence, Benon Asiimwe, Irene Andia
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Relationship between Virulence and Resistance among Gram-Negative Bacteria
Virginio Cepas, Sara M. Soto
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BMC Microbiology.2019;[Epub] CrossRef

Multilocus Sequence Analysis of Housekeeping Genes and Antigenic Determinant Genes in Bordetella pertussis Strains Isolated in Korea: Sang-Oun Jung, Yu Mi Moon, So-Hyeon Kim, Hwa Young Sung, Seung-Jik Kwon, Yeon Ho Kang, Jae Yon Yu; Osong Public Health Res Perspect. 2011;2(2):115-126. Published online June 30, 2011; DOI: https://doi.org/10.1016/j.phrp.2011.08.003

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3 Citations

Abstract PDF

Objectives
To confirm genotype diversities of clinical isolates of Bordetella pertussis and to evaluate the risk of pertussis outbreak in Korea.
Methods
Seven housekeeping genes and 10 antigenic determinant genes from clinical B. pertussis isolates were analyzed by Multilocus sequence typing (MLST).
Results
More variant pattern was observed in antigenic determinant genes. Especially, PtxS1 gene was the most variant gene; five genotypes were observed from eight global genotypes. In the bacterial type, the number of observed sequence types in the isolates was seven and the most frequent form was type 1 (79.6%). This major sequence type also showed a time-dependent transition pattern. Older isolates (1968 and 1975) showed type 1 and 6 in housekeeping genes and antigenic determinant genes, respectively. However, these were changed to type 2 and 1 in isolates 1999–2008. This transition was mainly attributed to genotype change of PtxS1 and Fim3 gene; the tendency of genotype change was to avoid vaccine-derived genotype. In addition, there was second transition in 2009. In this period, only the sequence type of antigenic determinant genes was changed to type 2. Based Upon Related Sequence Types (BURST) analysis confirmed that there were two clonal complexes (ACCI and ACCII) in the Korean isolates. Moreover, the recently increased sequence type was revealed as AST2 derived from AST 3 in ACCI.
Conclusions
Genotype changes in Korean distributing strains are still progressing and there was a specific driving force in antigenic determinant genes. Therefore continuous surveillance of genotype change of the distributing strains should be performed to confirm interrelationship of genotype change with vaccine immunity.

Citations

Citations to this article as recorded by

Characterization of Bordetella pertussis Strains Isolated from India
Shweta Alai, Manish Gautam, Sonali Palkar, Jitendra Oswal, Sunil Gairola, Dhiraj P. Dhotre
Pathogens.2022; 11(7): 794. CrossRef
Variation in Bordetella pertussis Susceptibility to Erythromycin and Virulence-Related Genotype Changes in China (1970-2014)
Ying Yang, Kaihu Yao, Xiang Ma, Wei Shi, Lin Yuan, Yonghong Yang, Daniela Flavia Hozbor
PLOS ONE.2015; 10(9): e0138941. CrossRef
Recent Trends of Antigenic Variation inBordetella pertussisIsolates in Korea
So-Hyun Kim, Jin Lee, Hwa Young Sung, Jae Yon Yu, Seong Han Kim, Mi Sun Park, Sang-Oun Jung
Journal of Korean Medical Science.2014; 29(3): 328. CrossRef

Distribution of Virulence Genes and Their Association of Serotypes in Pathogenic Escherichia coli Isolates From Diarrheal Patients in Korea: Seung-Hak Cho, Kyung-Hwan Oh, Seong-Han Kim, Hee-Bok Oh, Mi-Sun Park; Osong Public Health Res Perspect. 2010;1(1):29-35. Published online December 31, 2010; DOI: https://doi.org/10.1016/j.phrp.2010.12.008

3,028 View
16 Download
6 Citations

Abstract PDF

Objectives
To characterise the genetic and serological diversity of pathogenic Escherichia coli, we tested 111 E coli strains isolated from diarrhoeal patients in Korea between 2003 and 2006.
Methods
The isolates were tested through polymerase chain reaction (PCR) and slide agglutination method for the detection of virulence genes and serotypes, respectively. To compare the expression of Shiga toxin (stx)-1 and stx2 genes, real-time quantitative reverse-transcriptase PCR and rapid exprssion assay, reversed-passive latex agglutination, were performed.
Results
Forty-nine Shiga toxin-producing E coli (STEC) strains and 62 non-STEC strains, including 20 enteropathogenic E coli, 20 enterotoxigenic E coli, 20 enteroaggregative E coli, and 2 enteroinvasive E coli were randomly chosen from the strains isolated from diarrhoeal patients in Korea between 2003 and 2006. PCR analysis indicated that locus of enterocyte effacement pathogenicity island, that is, eaeA, espADB, and tir genes were present in STEC, enteropathogenic E coli, and enteroinvasive E coli. Quorum sensing-related gene luxS was detected in most of pathogenic E coli strains. Major serotypes of the STEC strains were O157 (26%) and O26 (20%), whereas the non-STEC strains possessed various serotypes. Especially, all the strains with serotype O157 carried stx2 and the tested virulence factors. Of the STEC strains, the data of real-time quantitative reverse-transcriptase PCR and reversed-passive latex agglutination tests showed that messenger RNA- and protein expression of stx2 gene were higher than those of stx1 gene.
Conclusion
Our results provide the epidemiological information regarding the trend of STEC and non-STEC infections in the general population and show the fundamental data in association of serotypes with virulence genes in diarrhoeagenic E coli strains from Korea.

Citations

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