Skip Navigation
Skip to contents

PHRP : Osong Public Health and Research Perspectives

OPEN ACCESS
SEARCH
Search

Search

Page Path
HOME > Search
4 "virulence factors"
Filter
Filter
Article category
Keywords
Publication year
Authors
Original Articles
Association between Beta-lactam Antibiotic Resistance and Virulence Factors in AmpC Producing Clinical Strains of P. aeruginosa
Sanaz Dehbashi, Hamed Tahmasebi, Mohammad Reza Arabestani
Osong Public Health Res Perspect. 2018;9(6):325-333.   Published online December 31, 2018
DOI: https://doi.org/10.24171/j.phrp.2018.9.6.06
  • 21,150 View
  • 129 Download
  • 12 Citations
AbstractAbstract PDF
Objectives

The purpose of this study was to determine the presence of IMP and OXA genes in clinical strains of Pseudomonas aeruginosa (P. aeruginosa) that are carriers of the ampC gene.

Methods

In this study, 105 clinical isolates of P. aeruginosa were collected. Antibiotic resistance patterns were determined using the disk diffusion method. The strains carrying AmpC enzymes were characterized by a combination disk method. Multiplex-PCR was used to identify resistance and virulence genes, chi-square test was used to determine the relationship between variables.

Results

Among 105 isolates of P. aeruginosa, the highest antibiotic resistance was to cefotaxime and aztreonam, and the least resistance was to colictin and ceftazidime. There were 49 isolates (46.66%) that showed an AmpC phenotype. In addition, the frequencies of the resistance genes were; OXA48 gene 85.2%, OXA199, 139 3.8%, OXA23 3.8%, OXA2 66.6%, OXA10 3.8%, OXA51 85.2% and OXA58 3.8%. The IMP27 gene was detected in 9 isolates (8.57%) and the IMP3.34 was detected in 11 isolates (10.47%). Other genes detected included; lasR (17.1%), lasB (18%) and lasA (26.6%). There was a significant relationship between virulence factors and the OX and IMP genes (p ≤ 0.05).

Conclusion

The relationship between antibiotic resistance and virulence factors observed in this study could play an important role in outbreaks associated with P. aeruginosa infections.

Citations

Citations to this article as recorded by  
  • Molecular epidemiology and collaboration of siderophore-based iron acquisition with surface adhesion in hypervirulent Pseudomonas aeruginosa isolates from wound infections
    Hamed Tahmasebi, Sanaz Dehbashi, Mona Nasaj, Mohammad Reza Arabestani
    Scientific Reports.2022;[Epub]     CrossRef
  • Decoding Genetic Features and Antimicrobial Susceptibility of Pseudomonas aeruginosa Strains Isolated from Bloodstream Infections
    Tomasz Bogiel, Dagmara Depka, Mateusz Rzepka, Agnieszka Mikucka
    International Journal of Molecular Sciences.2022; 23(16): 9208.     CrossRef
  • Prevalence of the Genes Associated with Biofilm and Toxins Synthesis amongst the Pseudomonas aeruginosa Clinical Strains
    Tomasz Bogiel, Dagmara Depka, Mateusz Rzepka, Joanna Kwiecińska-Piróg, Eugenia Gospodarek-Komkowska
    Antibiotics.2021; 10(3): 241.     CrossRef
  • A Comprehensive Study of the Relationship between the Production of β-Lactamase Enzymes and Iron/Siderophore Uptake Regulatory Genes in Clinical Isolates of Acinetobacter baumannii
    Mahyar Porbaran, Hamed Tahmasebi, MohammadReza Arabestani, Joseph Falkinham
    International Journal of Microbiology.2021; 2021: 1.     CrossRef
  • Regulation of virulence and β-lactamase gene expression in Staphylococcus aureus isolates: cooperation of two-component systems in bloodstream superbugs
    Sanaz Dehbashi, Hamed Tahmasebi, Behrouz Zeyni, Mohammad Reza Arabestani
    BMC Microbiology.2021;[Epub]     CrossRef
  • New approach to identify colistin‐resistant Pseudomonas aeruginosa by high‐resolution melting curve analysis assay
    H. Tahmasebi, S. Dehbashi, M.R. Arabestani
    Letters in Applied Microbiology.2020; 70(4): 290.     CrossRef
  • Resistant Pseudomonas aeruginosa carrying virulence genes in hospitalized patients with urinary tract infection from Sanandaj, west of Iran
    Safoura Derakhshan, Aslan Hosseinzadeh
    Gene Reports.2020; 20: 100675.     CrossRef
  • Prevalence and molecular typing of Metallo-β-lactamase-producing Pseudomonas aeruginosa with adhesion factors: A descriptive analysis of burn wounds isolates from Iran
    Hamed Tahmasebi, Sanaz Dehbashi, Mohammad Yousef Alikhani, Mahyar Porbaran, Mohammad Reza Arabestani
    Gene Reports.2020; 21: 100853.     CrossRef
  • Co-harboring of mcr-1 and β-lactamase genes in Pseudomonas aeruginosa by high-resolution melting curve analysis (HRMA): Molecular typing of superbug strains in bloodstream infections (BSI)
    Hamed Tahmasebi, Sanaz Dehbashi, Mohammad Reza Arabestani
    Infection, Genetics and Evolution.2020; 85: 104518.     CrossRef
  • Relationship between Biofilm Regulating Operons and Various Β-Lactamase Enzymes: Analysis of the Clinical Features of Infections caused by Non-Fermentative Gram-Negative Bacilli (Nfgnb) from Iran
    Mahyar Porbaran, Reza Habibipour
    Journal of Pure and Applied Microbiology.2020; 14(3): 1723.     CrossRef
  • Carbapenem-Resistant Pseudomonas aeruginosa Strains-Distribution of the Essential Enzymatic Virulence Factors Genes
    Tomasz Bogiel, Małgorzata Prażyńska, Joanna Kwiecińska-Piróg, Agnieszka Mikucka, Eugenia Gospodarek-Komkowska
    Antibiotics.2020; 10(1): 8.     CrossRef
  • Biofilm Formation and β-lactamase Enzymes: A Synergism Activity in Acinetobacter baumannii Isolated from Wound Infection
    Mahyar Porbaran, Reza Habibipour
    Journal of Advances in Medical and Biomedical Rese.2019; 27(125): 34.     CrossRef
Relationships between Virulence Factors and Antimicrobial Resistance among Escherichia coli Isolated from Urinary Tract Infections and Commensal Isolates in Tehran, Iran
Mohammad Reza Asadi Karam, Mehri Habibi, Saeid Bouzari
Osong Public Health Res Perspect. 2018;9(5):217-224.   Published online October 31, 2018
DOI: https://doi.org/10.24171/j.phrp.2018.9.5.02
  • 4,266 View
  • 104 Download
  • 18 Citations
AbstractAbstract PDF
Objectives

Uropathogenic Escherichia coli (UPEC) are the major cause of urinary tract infections (UTIs). Here, we determined whether sensitivity to antibiotics was related to the prevalence of iron scavenging genes, or to biofilm and hemolysis formation.

Methods

A total of 110 UPEC and 30 E coli isolates were collected from the urine of UTI patients and feces of healthy individuals without UTI, respectively. The presence of iron receptor genes and phenotypic properties were evaluated by polymerase chain reaction and phenotypic methods, respectively. Susceptibility to routine antibiotics was evaluated using the disc diffusion method.

Results

The prevalence of iron scavenging genes ranged from 21.8% (ireA) to 84.5% (chuA) in the UPEC. Resistance to ceftazidime and cefotaxime was significantly correlated with the presence of fyuA and iutA iron genes. Biofilm production was significantly associated with the prevalence of fyuA and hma iron genes. A higher degree of antibiotic resistance was exhibited by isolates that produced biofilms than by their non-biofilm producing counterparts.

Conclusion

Our study clearly indicates that biofilm production is associated with antibiotic resistance, and that iron receptors and hemolysin production also contribute to reduced antibiotic sensitivity. These results further our understanding of the role that these virulence factors play during UPEC pathogenesis, which in turn may be valuable for the development of novel treatment strategies against UTIs.

Citations

Citations to this article as recorded by  
  • Characterization of virulence determinants and phylogenetic background of multiple and extensively drug resistant Escherichia coli isolated from different clinical sources in Egypt
    Rana El-baz, Heba Shehta Said, Eman Salama Abdelmegeed, Rasha Barwa
    Applied Microbiology and Biotechnology.2022; 106(3): 1279.     CrossRef
  • A global systematic review and meta-analysis on correlation between biofilm producers and non-biofilm producers with antibiotic resistance in Uropathogenic Escherichia coli
    Mitra Garousi, Sina Monazami Tabar, Hosein Mirazi, Parnia Asgari, Paniz Sabeghi, Astireh Salehi, Azad Khaledi, Mohammad Ghenaat Pisheh Sanani, Hossein Karballaei Mirzahosseini
    Microbial Pathogenesis.2022; 164: 105412.     CrossRef
  • Virulence factors, antimicrobial resistance and the relationship between these characteristics in uropathogenic Escherichia coli
    Farzaneh Firoozeh, Mohammad Zibaei, Farzad Badmasti, Azad Khaledi
    Gene Reports.2022; 27: 101622.     CrossRef
  • Association between Virulence Factors and Antimicrobial Resistance of Klebsiella pneumoniae Clinical Isolates from North Kerala
    Ramya Kumaran, R.V. Geetha, Sabitha Baby
    Journal of Pure and Applied Microbiology.2022; 16(2): 867.     CrossRef
  • Characterization of virulence factors and antibiotic resistance pattern of uropathogenic Escherichia coli strains in a tertiary care center
    Naveen Kumar M, Sevitha Bhat, Archana Bhat K, Vishwas Saralaya, Shalini Shenoy Mulki
    F1000Research.2022; 11: 1163.     CrossRef
  • Antibiotic Resistance, Biofilm Formation and Sub-Inhibitory Hydrogen Peroxide Stimulation in Uropathogenic Escherichia coli
    Prabin Dawadi, Santosh Khanal, Tista Prasai Joshi, Sudeep KC, Reshma Tuladhar, Bijaya Laxmi Maharjan, Anjani Darai, Dev Raj Joshi
    Microbiology Insights.2022; 15: 117863612211352.     CrossRef
  • Insects, Rodents, and Pets as Reservoirs, Vectors, and Sentinels of Antimicrobial Resistance
    Willis Gwenzi, Nhamo Chaukura, Norah Muisa-Zikali, Charles Teta, Tendai Musvuugwa, Piotr Rzymski, Akebe Luther King Abia
    Antibiotics.2021; 10(1): 68.     CrossRef
  • Virulence genes and phylogenetic groups of uropathogenic Escherichia coli isolates from patients with urinary tract infection and uninfected control subjects: a case-control study
    Seyedeh Elham Rezatofighi, Mahsa Mirzarazi, Mansour Salehi
    BMC Infectious Diseases.2021;[Epub]     CrossRef
  • Virulence characterization and clonal analysis of uropathogenic Escherichia coli metallo-beta-lactamase-producing isolates
    Fatemeh Zangane Matin, Seyedeh Elham Rezatofighi, Mohammad Roayaei Ardakani, Mohammad Reza Akhoond, Fahimeh Mahmoodi
    Annals of Clinical Microbiology and Antimicrobials.2021;[Epub]     CrossRef
  • Antimicrobial Susceptibility and Detection of Virulence-Associated Genes in Escherichia coli Strains Isolated from Commercial Broilers
    Tímea Kocúreková, Lívia Karahutová, Dobroslava Bujňáková
    Antibiotics.2021; 10(11): 1303.     CrossRef
  • Evaluation of Biofilm Formation and Virulence Genes and Association with Antibiotic Resistance Patterns of Uropathogenic Escherichia coli Strains in Southwestern Iran
    Mostafa Boroumand, Asghar Sharifi, Mohammad Amin Ghatei, Mohsen Sadrinasab
    Jundishapur Journal of Microbiology.2021;[Epub]     CrossRef
  • Differences of virulence factors, and antimicrobial susceptibility according to phylogenetic group in uropathogenic Escherichia coli strains isolated from Korean patients
    Miri Hyun, Ji Yeon Lee, Hyun ah Kim
    Annals of Clinical Microbiology and Antimicrobials.2021;[Epub]     CrossRef
  • Changes in Bacterial Spectrum and Resistance Patterns Over Time in the Urine of Patients with Neurogenic Lower Urinary Tract Dysfunction Due to Spinal Cord Injury
    Jürgen Pannek, Carmen Kurmann, Jörg Krebs, Valentin Habermacher, Jens Wöllner
    Urologia Internationalis.2021; 105(5-6): 483.     CrossRef
  • A systematic review and meta-analysis of antibiotic resistance patterns, and the correlation between biofilm formation with virulence factors in uropathogenic E. coli isolated from urinary tract infections
    Fei Zhao, Huanxin Yang, Dezhong Bi, Azad Khaledi, Mingqi Qiao
    Microbial Pathogenesis.2020; 144: 104196.     CrossRef
  • A survey for phylogenetic relationship; presence of virulence genes and antibiotic resistance patterns of avian pathogenic and uropathogenic Escherichia coli isolated from poultry and humans in Yazd, Iran
    Mansoureh Bakhshi, Hengameh Zandi, Mehdi Fatahi Bafghi, Akram Astani, Vahid Reza Ranjbar, Mahmood Vakili
    Gene Reports.2020; 20: 100725.     CrossRef
  • Biofilm formation, antimicrobial susceptibility and virulence genes of Uropathogenic Escherichia coli isolated from clinical isolates in Uganda
    Paul Katongole, Fatuma Nalubega, Najjuka Christine Florence, Benon Asiimwe, Irene Andia
    BMC Infectious Diseases.2020;[Epub]     CrossRef
  • Relationship between Virulence and Resistance among Gram-Negative Bacteria
    Virginio Cepas, Sara M. Soto
    Antibiotics.2020; 9(10): 719.     CrossRef
  • Virulence factors of uropathogenic Escherichia coli (UPEC) and correlation with antimicrobial resistance
    Chhaya Shah, Ratna Baral, Bijay Bartaula, Lok Bahadur Shrestha
    BMC Microbiology.2019;[Epub]     CrossRef
Multilocus Sequence Analysis of Housekeeping Genes and Antigenic Determinant Genes in Bordetella pertussis Strains Isolated in Korea
Sang-Oun Jung, Yu Mi Moon, So-Hyeon Kim, Hwa Young Sung, Seung-Jik Kwon, Yeon Ho Kang, Jae Yon Yu
Osong Public Health Res Perspect. 2011;2(2):115-126.   Published online June 30, 2011
DOI: https://doi.org/10.1016/j.phrp.2011.08.003
  • 2,007 View
  • 13 Download
  • 3 Citations
AbstractAbstract PDF
Objectives
To confirm genotype diversities of clinical isolates of Bordetella pertussis and to evaluate the risk of pertussis outbreak in Korea.
Methods
Seven housekeeping genes and 10 antigenic determinant genes from clinical B. pertussis isolates were analyzed by Multilocus sequence typing (MLST).
Results
More variant pattern was observed in antigenic determinant genes. Especially, PtxS1 gene was the most variant gene; five genotypes were observed from eight global genotypes. In the bacterial type, the number of observed sequence types in the isolates was seven and the most frequent form was type 1 (79.6%). This major sequence type also showed a time-dependent transition pattern. Older isolates (1968 and 1975) showed type 1 and 6 in housekeeping genes and antigenic determinant genes, respectively. However, these were changed to type 2 and 1 in isolates 1999–2008. This transition was mainly attributed to genotype change of PtxS1 and Fim3 gene; the tendency of genotype change was to avoid vaccine-derived genotype. In addition, there was second transition in 2009. In this period, only the sequence type of antigenic determinant genes was changed to type 2. Based Upon Related Sequence Types (BURST) analysis confirmed that there were two clonal complexes (ACCI and ACCII) in the Korean isolates. Moreover, the recently increased sequence type was revealed as AST2 derived from AST 3 in ACCI.
Conclusions
Genotype changes in Korean distributing strains are still progressing and there was a specific driving force in antigenic determinant genes. Therefore continuous surveillance of genotype change of the distributing strains should be performed to confirm interrelationship of genotype change with vaccine immunity.

Citations

Citations to this article as recorded by  
  • Characterization of Bordetella pertussis Strains Isolated from India
    Shweta Alai, Manish Gautam, Sonali Palkar, Jitendra Oswal, Sunil Gairola, Dhiraj P. Dhotre
    Pathogens.2022; 11(7): 794.     CrossRef
  • Variation in Bordetella pertussis Susceptibility to Erythromycin and Virulence-Related Genotype Changes in China (1970-2014)
    Ying Yang, Kaihu Yao, Xiang Ma, Wei Shi, Lin Yuan, Yonghong Yang, Daniela Flavia Hozbor
    PLOS ONE.2015; 10(9): e0138941.     CrossRef
  • Recent Trends of Antigenic Variation inBordetella pertussisIsolates in Korea
    So-Hyun Kim, Jin Lee, Hwa Young Sung, Jae Yon Yu, Seong Han Kim, Mi Sun Park, Sang-Oun Jung
    Journal of Korean Medical Science.2014; 29(3): 328.     CrossRef
Distribution of Virulence Genes and Their Association of Serotypes in Pathogenic Escherichia coli Isolates From Diarrheal Patients in Korea
Seung-Hak Cho, Kyung-Hwan Oh, Seong-Han Kim, Hee-Bok Oh, Mi-Sun Park
Osong Public Health Res Perspect. 2010;1(1):29-35.   Published online December 31, 2010
DOI: https://doi.org/10.1016/j.phrp.2010.12.008
  • 2,107 View
  • 15 Download
  • 6 Citations
AbstractAbstract PDF
Objectives
To characterise the genetic and serological diversity of pathogenic Escherichia coli, we tested 111 E coli strains isolated from diarrhoeal patients in Korea between 2003 and 2006.
Methods
The isolates were tested through polymerase chain reaction (PCR) and slide agglutination method for the detection of virulence genes and serotypes, respectively. To compare the expression of Shiga toxin (stx)-1 and stx2 genes, real-time quantitative reverse-transcriptase PCR and rapid exprssion assay, reversed-passive latex agglutination, were performed.
Results
Forty-nine Shiga toxin-producing E coli (STEC) strains and 62 non-STEC strains, including 20 enteropathogenic E coli, 20 enterotoxigenic E coli, 20 enteroaggregative E coli, and 2 enteroinvasive E coli were randomly chosen from the strains isolated from diarrhoeal patients in Korea between 2003 and 2006. PCR analysis indicated that locus of enterocyte effacement pathogenicity island, that is, eaeA, espADB, and tir genes were present in STEC, enteropathogenic E coli, and enteroinvasive E coli. Quorum sensing-related gene luxS was detected in most of pathogenic E coli strains. Major serotypes of the STEC strains were O157 (26%) and O26 (20%), whereas the non-STEC strains possessed various serotypes. Especially, all the strains with serotype O157 carried stx2 and the tested virulence factors. Of the STEC strains, the data of real-time quantitative reverse-transcriptase PCR and reversed-passive latex agglutination tests showed that messenger RNA- and protein expression of stx2 gene were higher than those of stx1 gene.
Conclusion
Our results provide the epidemiological information regarding the trend of STEC and non-STEC infections in the general population and show the fundamental data in association of serotypes with virulence genes in diarrhoeagenic E coli strains from Korea.

Citations

Citations to this article as recorded by  
  • Comparative Analysis of Human and Animal E. coli: Serotyping, Antimicrobial Resistance, and Virulence Gene Profiling
    Mahmoud M. Bendary, Marwa I. Abdel-Hamid, Walaa A. Alshareef, Hanan M. Alshareef, Rasha A. Mosbah, Nasreen N. Omar, Mohammad M. Al-Sanea, Majid Alhomrani, Abdulhakeem S. Alamri, Walaa H. Moustafa
    Antibiotics.2022; 11(5): 552.     CrossRef
  • Antimicrobial peptide human β-defensin-2 improves in vitro cellular viability and reduces pro-inflammatory effects induced by enteroinvasive Escherichia coli in Caco-2 cells by inhibiting invasion and virulence factors’ expression
    Alessandra Fusco, Vittoria Savio, Brunella Perfetto, Roberto Mattina, Giovanna Donnarumma
    Frontiers in Cellular and Infection Microbiology.2022;[Epub]     CrossRef
  • Distribution of Pathogenicity Island Markers and H-Antigen Types of Escherichia coli O25b/ST131 Isolates from Patients with Urinary Tract Infection in Iran
    Masoumeh Rasoulinasab, Fereshteh Shahcheraghi, Mohammad Mehdi Feizabadi, Bahram Nikmanesh, Azade Hajihasani, Shahram Sabeti, Mohammad Mehdi Aslani
    Microbial Drug Resistance.2021; 27(3): 369.     CrossRef
  • Development and validation of a predictive model for pathogenic Escherichia coli in fresh‐cut produce
    You Jin Kim, Ju Yeon Park, Soo Hwan Suh, Mi‐Gyeong Kim, Hyo‐Sun Kwak, Soon Han Kim, Eun Jeong Heo
    Food Science & Nutrition.2021; 9(12): 6866.     CrossRef
  • In vitro antibacterial activity of poly (amidoamine)-G7 dendrimer
    Mitra Gholami, Rashin Mohammadi, Mohsen Arzanlou, Fakhraddin Akbari Dourbash, Ebrahim Kouhsari, Gharib Majidi, Seyed Mohsen Mohseni, Shahram Nazari
    BMC Infectious Diseases.2017;[Epub]     CrossRef
  • Occurrence of pathogenic Escherichia coli in commercially available fresh vegetable products in Korea
    Hyun Jung Kim, Minseon Koo, A-Ram Jeong, Seung-Youb Baek, Joon-Il Cho, Soon-Ho Lee, In-Gyun Hwang
    Journal of the Korean Society for Applied Biologic.2014; 57(3): 367.     CrossRef

PHRP : Osong Public Health and Research Perspectives