<sec>
<b>Objectives</b>
<p>The purpose of this study was to determine the presence of <italic>IMP</italic> and <italic>OXA</italic> genes in clinical strains of <italic>Pseudomonas aeruginosa</italic> (<italic>P. aeruginosa</italic>) that are carriers of the <italic>ampC</italic> gene.</p></sec>
<sec>
<b>Methods</b>
<p>In this study, 105 clinical isolates of <italic>P. aeruginosa</italic> were collected. Antibiotic resistance patterns were determined using the disk diffusion method. The strains carrying AmpC enzymes were characterized by a combination disk method. Multiplex-PCR was used to identify resistance and virulence genes, chi-square test was used to determine the relationship between variables.</p></sec>
<sec>
<b>Results</b>
<p>Among 105 isolates of <italic>P. aeruginosa</italic>, the highest antibiotic resistance was to cefotaxime and aztreonam, and the least resistance was to colictin and ceftazidime. There were 49 isolates (46.66%) that showed an AmpC phenotype. In addition, the frequencies of the resistance genes were; <italic>OXA48</italic> gene 85.2%, <italic>OXA199, 139</italic> 3.8%, <italic>OXA23</italic> 3.8%, <italic>OXA2</italic> 66.6%, <italic>OXA10</italic> 3.8%, <italic>OXA51</italic> 85.2% and <italic>OXA58</italic> 3.8%. The <italic>IMP27</italic> gene was detected in 9 isolates (8.57%) and the <italic>IMP3.34</italic> was detected in 11 isolates (10.47%). Other genes detected included; <italic>lasR</italic> (17.1%), <italic>lasB</italic> (18%) and <italic>lasA</italic> (26.6%). There was a significant relationship between virulence factors and the <italic>OX</italic> and <italic>IMP</italic> genes (<italic>p</italic> ≤ 0.05).</p></sec>
<sec>
<b>Conclusion</b>
<p>The relationship between antibiotic resistance and virulence factors observed in this study could play an important role in outbreaks associated with <italic>P. aeruginosa</italic> infections.</p></sec>
Citations
Citations to this article as recorded by
Prevalence -lactam resistant Pseudomonas aeruginosa strains isolated from chronic supportive otitis media infections: A single center survey in Eastern India Shakti Rath, Debidatta Singhsamanta, Debasmita Dubey, Saumya Ranjan Das IP International Journal of Medical Microbiology a.2024; 10(3): 258. CrossRef
Prevalence of colistin resistance in clinical isolates of Pseudomonas aeruginosa: a systematic review and meta-analysis Negar Narimisa, Abbasali Keshtkar, Leila Dadgar-Zankbar, Narjess Bostanghadiri, Yasaman Rouein Far, Soheila Shahroodian, Abed Zahedi Bialvaei, Shabnam Razavi Frontiers in Microbiology.2024;[Epub] CrossRef
Comparative bacteriome and antibiotic resistome analysis of water and sediment of the Ganga River of India Ankita Srivastava, Digvijay Verma World Journal of Microbiology and Biotechnology.2023;[Epub] CrossRef
Prevalence of different virulence factors and their association with antimicrobial resistance among Pseudomonas aeruginosa clinical isolates from Egypt Eva A. Edward, Marwa R. El Shehawy, Alaa Abouelfetouh, Elsayed Aboulmagd BMC Microbiology.2023;[Epub] CrossRef
Molecular epidemiology and collaboration of siderophore-based iron acquisition with surface adhesion in hypervirulent Pseudomonas aeruginosa isolates from wound infections Hamed Tahmasebi, Sanaz Dehbashi, Mona Nasaj, Mohammad Reza Arabestani Scientific Reports.2022;[Epub] CrossRef
Decoding Genetic Features and Antimicrobial Susceptibility of Pseudomonas aeruginosa Strains Isolated from Bloodstream Infections Tomasz Bogiel, Dagmara Depka, Mateusz Rzepka, Agnieszka Mikucka International Journal of Molecular Sciences.2022; 23(16): 9208. CrossRef
Prevalence of the Genes Associated with Biofilm and Toxins Synthesis amongst the Pseudomonas aeruginosa Clinical Strains Tomasz Bogiel, Dagmara Depka, Mateusz Rzepka, Joanna Kwiecińska-Piróg, Eugenia Gospodarek-Komkowska Antibiotics.2021; 10(3): 241. CrossRef
A Comprehensive Study of the Relationship between the Production of β-Lactamase Enzymes and Iron/Siderophore Uptake Regulatory Genes in Clinical Isolates of Acinetobacter baumannii Mahyar Porbaran, Hamed Tahmasebi, MohammadReza Arabestani, Joseph Falkinham International Journal of Microbiology.2021; 2021: 1. CrossRef
Regulation of virulence and β-lactamase gene expression in Staphylococcus aureus isolates: cooperation of two-component systems in bloodstream superbugs Sanaz Dehbashi, Hamed Tahmasebi, Behrouz Zeyni, Mohammad Reza Arabestani BMC Microbiology.2021;[Epub] CrossRef
New approach to identify colistin‐resistant
Pseudomonas aeruginosa
by high‐resolution melting curve analysis assay
H. Tahmasebi, S. Dehbashi, M.R. Arabestani Letters in Applied Microbiology.2020; 70(4): 290. CrossRef
Resistant Pseudomonas aeruginosa carrying virulence genes in hospitalized patients with urinary tract infection from Sanandaj, west of Iran Safoura Derakhshan, Aslan Hosseinzadeh Gene Reports.2020; 20: 100675. CrossRef
Prevalence and molecular typing of Metallo-β-lactamase-producing Pseudomonas aeruginosa with adhesion factors: A descriptive analysis of burn wounds isolates from Iran Hamed Tahmasebi, Sanaz Dehbashi, Mohammad Yousef Alikhani, Mahyar Porbaran, Mohammad Reza Arabestani Gene Reports.2020; 21: 100853. CrossRef
Co-harboring of mcr-1 and β-lactamase genes in Pseudomonas aeruginosa by high-resolution melting curve analysis (HRMA): Molecular typing of superbug strains in bloodstream infections (BSI) Hamed Tahmasebi, Sanaz Dehbashi, Mohammad Reza Arabestani Infection, Genetics and Evolution.2020; 85: 104518. CrossRef
Relationship between Biofilm Regulating Operons and Various Β-Lactamase Enzymes: Analysis of the Clinical Features of Infections caused by Non-Fermentative Gram-Negative Bacilli (Nfgnb) from Iran Mahyar Porbaran, Reza Habibipour Journal of Pure and Applied Microbiology.2020; 14(3): 1723. CrossRef
Carbapenem-Resistant Pseudomonas aeruginosa Strains-Distribution of the Essential Enzymatic Virulence Factors Genes Tomasz Bogiel, Małgorzata Prażyńska, Joanna Kwiecińska-Piróg, Agnieszka Mikucka, Eugenia Gospodarek-Komkowska Antibiotics.2020; 10(1): 8. CrossRef
Biofilm Formation and β-lactamase Enzymes: A Synergism Activity in Acinetobacter baumannii Isolated from Wound Infection Mahyar Porbaran, Reza Habibipour Journal of Advances in Medical and Biomedical Rese.2019; 27(125): 34. CrossRef
<sec>
<b>Objectives</b>
<p>Uropathogenic <italic>Escherichia coli</italic> (UPEC) are the major cause of urinary tract infections (UTIs). Here, we determined whether sensitivity to antibiotics was related to the prevalence of iron scavenging genes, or to biofilm and hemolysis formation.</p></sec>
<sec>
<b>Methods</b>
<p>A total of 110 UPEC and 30 <italic>E coli</italic> isolates were collected from the urine of UTI patients and feces of healthy individuals without UTI, respectively. The presence of iron receptor genes and phenotypic properties were evaluated by polymerase chain reaction and phenotypic methods, respectively. Susceptibility to routine antibiotics was evaluated using the disc diffusion method.</p></sec>
<sec>
<b>Results</b>
<p>The prevalence of iron scavenging genes ranged from 21.8% (<italic>ireA</italic>) to 84.5% (<italic>chuA</italic>) in the UPEC. Resistance to ceftazidime and cefotaxime was significantly correlated with the presence of <italic>fyuA</italic> and <italic>iutA</italic> iron genes. Biofilm production was significantly associated with the prevalence of <italic>fyuA</italic> and <italic>hma</italic> iron genes. A higher degree of antibiotic resistance was exhibited by isolates that produced biofilms than by their non-biofilm producing counterparts.</p></sec>
<sec>
<b>Conclusion</b>
<p>Our study clearly indicates that biofilm production is associated with antibiotic resistance, and that iron receptors and hemolysin production also contribute to reduced antibiotic sensitivity. These results further our understanding of the role that these virulence factors play during UPEC pathogenesis, which in turn may be valuable for the development of novel treatment strategies against UTIs.</p></sec>
Citations
Citations to this article as recorded by
Escherichia coli: An arduous voyage from commensal to Antibiotic-resistance Shiela Chetri Microbial Pathogenesis.2025; 198: 107173. CrossRef
Third‐Generation Cephalosporin‐Resistant Uropathogenic Escherichia coli From Community‐ and Hospital‐Acquired Infections Show High Level of Antibiotic Resistance and Specific Virulence Traits Amina Bougouizi, Astri Dwyanti Tagueha, Daniela Scribano, Zohra Chekroud, Zahrat el Imen Lamraoui, Lucia Nencioni, Cecilia Ambrosi, Hamza Rahab, Divakar Sharma Canadian Journal of Infectious Diseases and Medica.2025;[Epub] CrossRef
Correlation between antimicrobial resistance, biofilm formation, and virulence determinants in uropathogenic Escherichia coli from Egyptian hospital Sara A. Alshaikh, Tarek El-banna, Fatma Sonbol, Mahmoud H. Farghali Annals of Clinical Microbiology and Antimicrobials.2024;[Epub] CrossRef
Multidrug resistance in pathogenic Escherichia coli isolates from urinary tract infections in dogs, Spain Ana Abad-Fau, Eloisa Sevilla, Ainara Oro, Inmaculada Martín-Burriel, Bernardino Moreno, Mariano Morales, Rosa Bolea Frontiers in Veterinary Science.2024;[Epub] CrossRef
Resistant Escherichia coli isolated from wild mammals from two rescue and rehabilitation centers in Costa Rica: characterization and public health relevance Rita Fernandes, Raquel Abreu, Isa Serrano, Roger Such, Encarnación Garcia-Vila, Sandy Quirós, Eva Cunha, Luís Tavares, Manuela Oliveira Scientific Reports.2024;[Epub] CrossRef
Progress toward a vaccine for extraintestinal pathogenic
E. coli
(ExPEC) II: efficacy of a toxin-autotransporter dual antigen approach
Yikun Xing, Justin R. Clark, James D. Chang, Jacob J. Zulk, Dylan M. Chirman, Felipe-Andres Piedra, Ellen E. Vaughan, Haroldo J. Hernandez Santos, Kathryn A. Patras, Anthony W. Maresso, Kimberly A. Kline Infection and Immunity.2024;[Epub] CrossRef
Escherichia coli isolated from pyometra and cystitis in the same animal exhibit a wide phenotypic similarity Camila Azevedo Moni, Maria Eduarda Dias, Cassiane Elisabete Lopes, Franciele Maboni Siqueira Journal of Applied Microbiology.2024;[Epub] CrossRef
Molecular Evaluation of Aminoglycoside Resistance and Biofilm Formation Potential in Escherichia coli Isolates Collected from Hospitalized Patients Sousan Akrami, Saeed Khoshnood, Maryam Koupaei, Effat Abbasi Montazeri, Hossein Meghdadi, Morteza Saki, Moloudsadat Motahar, Sara Masihzadeh, Sara Daneshfar, Marjan Abdi, Zahra Farshadzadeh Jundishapur Journal of Microbiology.2024;[Epub] CrossRef
Detection and Molecular Characterization of Escherichia coli from Wastewater Environments in Two University Campuses in Nigeria Olubunmi Marvelous Emurotu, Chukwudi Anyanwu, Cornelius Arome Omatola, Ruth Foluke Aminu, Joseph Oyiguh Abraham, Gilbert Karngong Nfor, Sunday Ocholi Samson, S’fiso Thuthukani Gumbi Frontiers in Bioscience-Elite.2024;[Epub] CrossRef
Uropathogenic Escherichia coli (UPEC)-Associated Urinary Tract Infections: The Molecular Basis for Challenges to Effective Treatment Shane Whelan, Brigid Lucey, Karen Finn Microorganisms.2023; 11(9): 2169. CrossRef
Susceptibility and Virulence of Enterobacteriaceae Isolated from Urinary Tract Infections in Benin Funkè F. Assouma, Haziz Sina, Tomabu Adjobimey, Agossou Damien Pacôme Noumavo, Akim Socohou, Bawa Boya, Ange D. Dossou, Lauriane Akpovo, Basile Boni Saka Konmy, Jacques F. Mavoungou, Adolphe Adjanohoun, Lamine Baba-Moussa Microorganisms.2023; 11(1): 213. CrossRef
Association Between Uropathogenic Escherichia coli Virulence Genes and Severity of Infection and Resistance to Antibiotics Sofía Alejandra Fonseca-Martínez, Ruth Aralí Martínez-Vega, Ana Elvira Farfán-García, Clara Isabel González Rugeles, Libeth Yajaira Criado-Guerrero Infection and Drug Resistance.2023; Volume 16: 3707. CrossRef
Incidence of biofilms among the multidrug resistant E. coli, isolated from urinary tract infections in the Nilgiris district, South India A. P. Cardiliya, M. J. N. Chandrasekar, M. J. Nanjan Brazilian Journal of Microbiology.2023; 54(3): 1809. CrossRef
Correlation of biofilm formation, virulence factors, and phylogenetic groups among Escherichia coli strains causing urinary tract infection: A global systematic review and meta-analysis Hossein Karballaei Mirzahosseini, Farhad Najmeddin, Atabak Najafi, Arezoo Ahmadi, Hamidreza Sharifnia, Azad Khaledi, Mojtaba Mojtahedzadeh Journal of Research in Medical Sciences.2023;[Epub] CrossRef
Diabetic Foot Osteomyelitis Caused by Co-Infection with Methicillin-Resistant Staphylococcus aureus and Multidrug-Resistant Extended-Spectrum ß-Lactamase-Producing Escherichia coli: A Case Report Shiori Kitaya, Chieko Miura, Ayano Suzuki, Yoshimichi Imai, Koichi Tokuda, Hajime Kanamori Applied Microbiology.2023; 3(3): 1046. CrossRef
Fluoroquinolone resistance determinants in carbapenem-resistant Escherichia coli isolated from urine clinical samples in Thailand Parichart Boueroy, Peechanika Chopjitt, Rujirat Hatrongjit, Masatomo Morita, Yo Sugawara, Yukihiro Akeda, Tetsuya Iida, Shigeyuki Hamada, Anusak Kerdsin PeerJ.2023; 11: e16401. CrossRef
Characterization of virulence determinants and phylogenetic background of multiple and extensively drug resistant Escherichia coli isolated from different clinical sources in Egypt Rana El-baz, Heba Shehta Said, Eman Salama Abdelmegeed, Rasha Barwa Applied Microbiology and Biotechnology.2022; 106(3): 1279. CrossRef
A global systematic review and meta-analysis on correlation between biofilm producers and non-biofilm producers with antibiotic resistance in Uropathogenic Escherichia coli Mitra Garousi, Sina Monazami Tabar, Hosein Mirazi, Parnia Asgari, Paniz Sabeghi, Astireh Salehi, Azad Khaledi, Mohammad Ghenaat Pisheh Sanani, Hossein Karballaei Mirzahosseini Microbial Pathogenesis.2022; 164: 105412. CrossRef
Virulence factors, antimicrobial resistance and the relationship between these characteristics in uropathogenic Escherichia coli Farzaneh Firoozeh, Mohammad Zibaei, Farzad Badmasti, Azad Khaledi Gene Reports.2022; 27: 101622. CrossRef
Association between Virulence Factors and Antimicrobial Resistance of Klebsiella pneumoniae Clinical Isolates from North Kerala Ramya Kumaran, R.V. Geetha, Sabitha Baby Journal of Pure and Applied Microbiology.2022; 16(2): 867. CrossRef
Characterization of virulence factors and antibiotic resistance pattern of uropathogenic Escherichia coli strains in a tertiary care center Naveen Kumar M, Sevitha Bhat, Archana Bhat K, Vishwas Saralaya, Shalini Shenoy Mulki F1000Research.2022; 11: 1163. CrossRef
Characterization of virulence factors and antibiotic resistance pattern of uropathogenic Escherichia coli strains in a tertiary care center Naveen Kumar M, Sevitha Bhat, Archana Bhat K, Vishwas Saralaya, Shalini Shenoy Mulki F1000Research.2022; 11: 1163. CrossRef
Insects, Rodents, and Pets as Reservoirs, Vectors, and Sentinels of Antimicrobial Resistance Willis Gwenzi, Nhamo Chaukura, Norah Muisa-Zikali, Charles Teta, Tendai Musvuugwa, Piotr Rzymski, Akebe Luther King Abia Antibiotics.2021; 10(1): 68. CrossRef
Virulence genes and phylogenetic groups of uropathogenic Escherichia coli isolates from patients with urinary tract infection and uninfected control subjects: a case-control study Seyedeh Elham Rezatofighi, Mahsa Mirzarazi, Mansour Salehi BMC Infectious Diseases.2021;[Epub] CrossRef
Virulence characterization and clonal analysis of uropathogenic Escherichia coli metallo-beta-lactamase-producing isolates Fatemeh Zangane Matin, Seyedeh Elham Rezatofighi, Mohammad Roayaei Ardakani, Mohammad Reza Akhoond, Fahimeh Mahmoodi Annals of Clinical Microbiology and Antimicrobials.2021;[Epub] CrossRef
Antimicrobial Susceptibility and Detection of Virulence-Associated Genes in Escherichia coli Strains Isolated from Commercial Broilers Tímea Kocúreková, Lívia Karahutová, Dobroslava Bujňáková Antibiotics.2021; 10(11): 1303. CrossRef
Evaluation of Biofilm Formation and Virulence Genes and Association with Antibiotic Resistance Patterns of Uropathogenic Escherichia coli Strains in Southwestern Iran Mostafa Boroumand, Asghar Sharifi, Mohammad Amin Ghatei, Mohsen Sadrinasab Jundishapur Journal of Microbiology.2021;[Epub] CrossRef
Differences of virulence factors, and antimicrobial susceptibility according to phylogenetic group in uropathogenic Escherichia coli strains isolated from Korean patients Miri Hyun, Ji Yeon Lee, Hyun ah Kim Annals of Clinical Microbiology and Antimicrobials.2021;[Epub] CrossRef
Changes in Bacterial Spectrum and Resistance Patterns Over Time in the Urine of Patients with Neurogenic Lower Urinary Tract Dysfunction Due to Spinal Cord Injury Jürgen Pannek, Carmen Kurmann, Jörg Krebs, Valentin Habermacher, Jens Wöllner Urologia Internationalis.2021; 105(5-6): 483. CrossRef
A systematic review and meta-analysis of antibiotic resistance patterns, and the correlation between biofilm formation with virulence factors in uropathogenic E. coli isolated from urinary tract infections Fei Zhao, Huanxin Yang, Dezhong Bi, Azad Khaledi, Mingqi Qiao Microbial Pathogenesis.2020; 144: 104196. CrossRef
A survey for phylogenetic relationship; presence of virulence genes and antibiotic resistance patterns of avian pathogenic and uropathogenic Escherichia coli isolated from poultry and humans in Yazd, Iran Mansoureh Bakhshi, Hengameh Zandi, Mehdi Fatahi Bafghi, Akram Astani, Vahid Reza Ranjbar, Mahmood Vakili Gene Reports.2020; 20: 100725. CrossRef
Biofilm formation, antimicrobial susceptibility and virulence genes of Uropathogenic Escherichia coli isolated from clinical isolates in Uganda Paul Katongole, Fatuma Nalubega, Najjuka Christine Florence, Benon Asiimwe, Irene Andia BMC Infectious Diseases.2020;[Epub] CrossRef
Relationship between Virulence and Resistance among Gram-Negative Bacteria Virginio Cepas, Sara M. Soto Antibiotics.2020; 9(10): 719. CrossRef
Virulence factors of uropathogenic Escherichia coli (UPEC) and correlation with antimicrobial resistance Chhaya Shah, Ratna Baral, Bijay Bartaula, Lok Bahadur Shrestha BMC Microbiology.2019;[Epub] CrossRef
<b>Objectives</b><br/>
To confirm genotype diversities of clinical isolates of <i>Bordetella pertussis</i> and to evaluate the risk of pertussis outbreak in Korea.<br/><b>Methods</b><br/>
Seven housekeeping genes and 10 antigenic determinant genes from clinical <i>B. pertussis</i> isolates were analyzed by Multilocus sequence typing (MLST).<br/><b>Results</b><br/>
More variant pattern was observed in antigenic determinant genes. Especially, <i>PtxS1</i> gene was the most variant gene; five genotypes were observed from eight global genotypes. In the bacterial type, the number of observed sequence types in the isolates was seven and the most frequent form was type 1 (79.6%). This major sequence type also showed a time-dependent transition pattern. Older isolates (1968 and 1975) showed type 1 and 6 in housekeeping genes and antigenic determinant genes, respectively. However, these were changed to type 2 and 1 in isolates 1999–2008. This transition was mainly attributed to genotype change of <i>PtxS1</i> and <i>Fim3</i> gene; the tendency of genotype change was to avoid vaccine-derived genotype. In addition, there was second transition in 2009. In this period, only the sequence type of antigenic determinant genes was changed to type 2. Based Upon Related Sequence Types (BURST) analysis confirmed that there were two clonal complexes (ACCI and ACCII) in the Korean isolates. Moreover, the recently increased sequence type was revealed as AST2 derived from AST 3 in ACCI.<br/><b>Conclusions</b><br/>
Genotype changes in Korean distributing strains are still progressing and there was a specific driving force in antigenic determinant genes. Therefore continuous surveillance of genotype change of the distributing strains should be performed to confirm interrelationship of genotype change with vaccine immunity.
Citations
Citations to this article as recorded by
Pertussis in the Post-COVID-19 Era: Resurgence, Diagnosis, and Management Hyun Mi Kang, Taek-Jin Lee, Su Eun Park, Soo-Han Choi Infection & Chemotherapy.2025; 57(1): 13. CrossRef
Characterization of Bordetella pertussis Strains Isolated from India Shweta Alai, Manish Gautam, Sonali Palkar, Jitendra Oswal, Sunil Gairola, Dhiraj P. Dhotre Pathogens.2022; 11(7): 794. CrossRef
Variation in Bordetella pertussis Susceptibility to Erythromycin and Virulence-Related Genotype Changes in China (1970-2014) Ying Yang, Kaihu Yao, Xiang Ma, Wei Shi, Lin Yuan, Yonghong Yang, Daniela Flavia Hozbor PLOS ONE.2015; 10(9): e0138941. CrossRef
Recent Trends of Antigenic Variation inBordetella pertussisIsolates in Korea So-Hyun Kim, Jin Lee, Hwa Young Sung, Jae Yon Yu, Seong Han Kim, Mi Sun Park, Sang-Oun Jung Journal of Korean Medical Science.2014; 29(3): 328. CrossRef
<b>Objectives</b><br/>
To characterise the genetic and serological diversity of pathogenic <i>Escherichia coli</i>, we tested 111 <i>E coli</i> strains isolated from diarrhoeal patients in Korea between 2003 and 2006.<br/><b>Methods</b><br/>
The isolates were tested through polymerase chain reaction (PCR) and slide agglutination method for the detection of virulence genes and serotypes, respectively. To compare the expression of Shiga toxin (<i>stx</i>)-1 and <i>stx2</i> genes, real-time quantitative reverse-transcriptase PCR and rapid exprssion assay, reversed-passive latex agglutination, were performed.<br/><b>Results</b><br/>
Forty-nine Shiga toxin-producing <i>E coli</i> (STEC) strains and 62 non-STEC strains, including 20 enteropathogenic <i>E coli</i>, 20 enterotoxigenic <i>E coli</i>, 20 enteroaggregative <i>E coli</i>, and 2 enteroinvasive <i>E coli</i> were randomly chosen from the strains isolated from diarrhoeal patients in Korea between 2003 and 2006. PCR analysis indicated that locus of enterocyte effacement pathogenicity island, that is, <i>eae</i>A, <i>esp</i>ADB, and <i>tir</i> genes were present in STEC, enteropathogenic <i>E coli</i>, and enteroinvasive <i>E coli</i>. Quorum sensing-related gene <i>lux</i>S was detected in most of pathogenic <i>E coli</i> strains. Major serotypes of the STEC strains were O157 (26%) and O26 (20%), whereas the non-STEC strains possessed various serotypes. Especially, all the strains with serotype O157 carried <i>stx</i>2 and the tested virulence factors. Of the STEC strains, the data of real-time quantitative reverse-transcriptase PCR and reversed-passive latex agglutination tests showed that messenger RNA- and protein expression of <i>stx</i>2 gene were higher than those of <i>stx</i>1 gene.<br/><b>Conclusion</b><br/>
Our results provide the epidemiological information regarding the trend of STEC and non-STEC infections in the general population and show the fundamental data in association of serotypes with virulence genes in diarrhoeagenic <i>E coli</i> strains from Korea.
Citations
Citations to this article as recorded by
Comparative Analysis of Human and Animal E. coli: Serotyping, Antimicrobial Resistance, and Virulence Gene Profiling Mahmoud M. Bendary, Marwa I. Abdel-Hamid, Walaa A. Alshareef, Hanan M. Alshareef, Rasha A. Mosbah, Nasreen N. Omar, Mohammad M. Al-Sanea, Majid Alhomrani, Abdulhakeem S. Alamri, Walaa H. Moustafa Antibiotics.2022; 11(5): 552. CrossRef
Antimicrobial peptide human β-defensin-2 improves in vitro cellular viability and reduces pro-inflammatory effects induced by enteroinvasive Escherichia coli in Caco-2 cells by inhibiting invasion and virulence factors’ expression Alessandra Fusco, Vittoria Savio, Brunella Perfetto, Roberto Mattina, Giovanna Donnarumma Frontiers in Cellular and Infection Microbiology.2022;[Epub] CrossRef
Distribution of Pathogenicity Island Markers and H-Antigen Types of Escherichia coli O25b/ST131 Isolates from Patients with Urinary Tract Infection in Iran Masoumeh Rasoulinasab, Fereshteh Shahcheraghi, Mohammad Mehdi Feizabadi, Bahram Nikmanesh, Azade Hajihasani, Shahram Sabeti, Mohammad Mehdi Aslani Microbial Drug Resistance.2021; 27(3): 369. CrossRef
Development and validation of a predictive model for pathogenic Escherichia coli in fresh‐cut produce You Jin Kim, Ju Yeon Park, Soo Hwan Suh, Mi‐Gyeong Kim, Hyo‐Sun Kwak, Soon Han Kim, Eun Jeong Heo Food Science & Nutrition.2021; 9(12): 6866. CrossRef
Occurrence of pathogenic Escherichia coli in commercially available fresh vegetable products in Korea Hyun Jung Kim, Minseon Koo, A-Ram Jeong, Seung-Youb Baek, Joon-Il Cho, Soon-Ho Lee, In-Gyun Hwang Journal of the Korean Society for Applied Biologic.2014; 57(3): 367. CrossRef