1. Introduction

The prevention and control of communicable diseases depend on the natural history of diseases. Vector-borne diseases can be prevented and controlled mainly by vector control [1]. In particular, chikungunya and dengue can be controlled only by effective vector control because there is neither a specific treatment nor an effective vaccine to reduce or prevent the disease in the community [2]. Among the vector control practices in India, chemical control is given more priority than others. As far as chikungunya and dengue, Aedes aegypti and A albopictus are primary vectors [3]. These are usually bred in wet containers and, hence, source reduction is the best way of vector control rather than chemical control [4]. Chikungunya transmission has been studied less extensively compared to malaria, filariasis, and dengue. Dengue surveillance has vector indices, namely, Breteau (number of infested containers per 100 premises), house (percent houses infested), and container (percent wet containers infested) [5]. Although these larval indices are generally considered to have a poor relationship with adult abundance; therefore, the definition of dengue risk is considered to be questionable [1,6]. Despite that, these indices were employed in the recent episode of chikungunya transmission in Tamil Nadu for sustainable management of Aedes species mosquitoes. A methodology was searched to conduct rapid Aedes survey among human dwellings of the Chidambaram urban area of the Cuddalore District to identify the presence of Aedes larvae quickly to reduce its sources with the help of a premises condition index (PCI) [6,7]. In this study, PCI variables, types of houses, hygienic condition of yards, and degree of shadow were employed to identify the presence of Aedes larvae to conduct surveys rapidly with a minimum number of workers.

2. Materials and Methods

2.1. Study area

The study area Chidambaram is situated in the Cuddalore District of Tamil Nadu state, India. Its latitude is 11°24’N and the longitude is 79°42’E. Chidambaram is a municipal town and it has 33 wards with the population of 54,000. A total of 33 streets were selected from 33 wards. The selection of the street was made each lot. All the houses situated in a street were subjected to this survey. The survey was conducted during the peak chikungunya transmission months of September to October 2006. As these months fell in the wet season, so A. spp were expected to be in great abundance.

Table 1.

Distribution of positive premise by house condition

House condition	Positive premises, no.	Percentage	Negative premises, no.	Percentage	Total
Well maintained	43	4.6	888	95.4	931
Moderately maintained	73	9.6	686	90.4	759
Not well maintained	16	13.0	107	87.0	123
Total	132		1,681		1,813

2.2. Premise variables

The house condition, the yard condition, and the degree of shadow were taken for this survey. Prior to collecting the details of PCI variables such as house condition, the yard condition, and the degree of shade, these were familiarized to the investigators with their definition. House (it is a human living place. Its condition is being determined by the periodical maintenance of inmates), yard (this is some space available either in front of the house or at the back of the house) and the degree of shadow is determined based on the vegetation in and around the house. These are defined as adopting of the reference [6,8]. The house conditions were classified as 1 = well maintained house (e.g., newly painted, or new house); 2 = moderately well, a maintained house; and 3 = not a well-maintained house (e.g., paint peeling, broken items visible, dilapidated, especially if old). The yard conditions were classified as 1 = tidy yard (e.g well-maintained gardens and debris and litter free); 2 = moderately tidy yard; and 3 = untidy yard, trash abundant, and overgrown vegetation. Shade conditions were classified as 1 = very little or no shade (<25%), e.g. no major trees or shrubbery; 2 = some shade with major trees and vegetation (50%) and shady, e.g., large trees, shrubbery, green house, or shade cloth used; and 3 = having major trees and shrubs (75%). Categories 1 and 3 were relatively easy to score because they represented opposites. Category 2 was often selected by default. A presurvey was conducted in the residential areas of the Chidambaram urban area so that the features of these variables would remain in the investigators’ minds. The outcome of the presurvey was compared with the actual survey, which was conducted by the skilled investigators, namely senior entomologists and entomological assistants. This was repeated until the outcome was the same. For this survey, lab assistants, field assistants, and field workers of the National Filarial Control Programme in Chidambaram were employed.

2.3. Container data

A total of 1,813 premises were inspected from September to October 2006 in Chidambaram Town of Cuddalore district in Tamil Nadu, India, and scored as indicated above. The presence of immature of A spp mosquitoes and the number of positive containers was recorded for each.

Table 2.

Distribution of positive premises by yard condition

Yard condition	Positive premises, no.	Percentage	Negative premises, no.	Percentage	Total
Tidy yard	42	4.6	880	95.4	922
Moderately tidy	67	9.3	652	90.7	719
Untidy	23	13.4	149	86.6	172
Total	132		1,681		1,813

2.4. Statistical methods

Statistical methods such as the Chi-square test, trend Chi-square, and logistic regression analysis were used. To examine the association of wet containers that were positive for A spp with variable premises, univariate and multivariate analysis were performed. The unadjusted and adjusted odds ratio was computed for positivity of A spp with variable premise. All analyses were twotailed and a p value of <0.05 was considered statistically significant. Statistical analysis was performed by using SPSS version (SPSS IBM Company, Chicago-3, USA) 10.0 packages.

3. Results

This study was conducted in the Chidambaram urban area of Cuddalore District, Tamil Nadu, India, during the months of September to October 2006. A total of 1813 household were surveyed and 132 premise households were found to be positive. The distribution of positive premises by house conditions and shade conditions is described in Tables 1 to 3. It was observed that the positive premise were significantly higher (p < 0.05) in houses that were not well maintained, with untidy yard conditions, and very little shade.

The PCI was computed based on three premise variables, such as house condition, yard condition, and shade conditions. There was a positive correlation between PCI and the proportion of positive premise (r = 0.171; p = 0.01) and with the number of positive containers in the premise (r = 0.153; p = 0.01).

A significant increasing trend was observed between the proportion of positive premises and the PCI score (p = 0.001; Table 4). It was observed that there was a 5.6 times greater probability of detecting positive

Table 3.

Distribution of positive premises by shade condition

Shade condition	Positive premises, no.	Percentage	Negative premises, no.	Percentage	Total
Very little shade	42	4.0	1,020	96.0	1,062
Some shade	45	9.6	423	90.4	468
More shade	45	15.9	238	84.1	283
Total	132		1,681		1,813

premises in highest score compares with the lowest score index.

These three variables were used simultaneously to find any observed association with positive premises and was examined by employing logistic regression analysis. It was observed that the degree of shade shows a significant association (p = 0.001) with positive premises when adjusted for house and yard conditions. In fact, the possibility of detecting positive premises was four times higher in the premises with more shade (75%) compared with premises with very little shade (25%; Table 5).

4. Discussion

Several communicable diseases are endemic in India. An effective surveillance system is essential for planning, implementation, and monitoring of disease control programs. Many of these diseases have seasonal and cyclic trends, which can be detected through the surveillance system [9]. These diseases can also cause outbreaks with the potential to spread rapidly and cause many deaths in general [10]. The recent outbreak of chikungunya in southern parts of India taught many lessons about its control and prevention. Among them, vector control activities employed with the political commitments of Government of Tamil Nadu and the Government of India involving all the components of Primary Health Centers had been received more attention as it was exclusively dependent on the source reduction of Aedes species larvae in human dwellings than chemotherapy. It is known that there is no treatment available to destroy the causative organism, the chik virus. In this situation, as per the guidelines of WHO [2], entomologic surveillance was used to

Table 4.

The proportion of positive premises and the premises condition index score

Total score	Houses surveyed, no.	House positive	Container positive	Proportion of positive houses	Percentage	Mean no. of containers	Percentage
3	589	25	50	0.04	4.24	0.08	8.49
4	273	7	24	0.03	2.56	0.09	8.79
5	416	23	37	0.06	5.53	0.09	8.89
6	280	33	71	0.12	11.79	0.25	25.36
7	153	26	79	0.17	16.99	0.52	51.63
8	67	11	25	0.16	16.42	0.37	37.31
9	35	7	15	0.20	20.00	0.43	42.86
Total	1,813	132	301

determine changes in the geographic distribution and density of the vectors, evaluation of the control program, obtain relative measurements of the vector population overtime, and facilitate appropriate and timely decision regarding intervention. Besides, a number of methods are available for detecting or monitoring immature and adult populations through the selection of appropriate sampling methods depend on the surveillance objectives, levels of infestation, available funding, and skills of personnel [11]. Further, the guidance of the choice of surveillance methods for A aegypti are comprised of the following: (1) baseline infestation surveys; (2) control program that monitors low infestation level of <5% house index; (3) control program that monitors the level >5% house index; (4) surveillance against reinfestation; and (5) verification of eradication and evaluation of the control methods. Among the methods on vector control, larval survey, collection on humans, collection of resting mosquitoes, ovitraps, tyre, larvitraps, and insecticide susceptibility tests are appropriate [12]. Scrutinizing these methods of evaluation, larval survey is the foremost as it employs for complete source reduction of Aedes species and hence the PCI was tested on the

Table 5.

Adjusted odds ratio for positive premise according to house, yard, and shade conditions

Type of condition	Adjusted odds ratio	95% confidence interval for adjusted odds ratio	p
House
Well	1.00
Moderate	0.57	0.24-1.30	0.181
Not well	0.83	0.41-1.66	0.591
Yard
Tidy	1.00
Moderate	1.30	0.73-2.31	0.370
Untidy	1.28	0.58-2.76	0.538
Shade
Very little	1.00
Some	2.22	1.42-3.47	<0.001
More	3.82	2.38-6.12	<0.001

presence of Aedes larvae species through the correlation of its variables house, yard condition and degrees of shadows for its application in rapid survey of Aedes species apart from HI, container Index and breateau Index have been described and we currently use them to monitor Aedes species population for dengue virus transmission.

In the present study, three variables–house, yard, and degrees of shadow–were used simultaneously to find any observed association with positive premises was examined by employing logistic regression analysis. Among the premise variables, we observed the degree of shade association is more with positivity of Aedes species when adjusted for house and yard. Also it is noted the probability of detecting positive premises was four times higher in the premises with 75% shade compared with the premise with 25% shade. Toward the same line of observation, the morbidity status of chikungunya was also examined that signs and symptoms of it were observed in dwellings with sources of Aedes breeding and 75% degree of shadow irrespective of house and yard condition (data unpublished). Hence, the degree of shadow could be one of the factors to be considered for developing PCI index. Going through the literature, it was known that shade level can be accurately identified even from the aerial with the help of both color and infrared images and the positivity of larvae was compared with variables (house, yard, and degrees of shadow). It was known that there was no much variation in the prediction of positivity using all these variables put together rather than degrees of shades alone [8]. Similarly [6], Tun-Lin stated that the PCI was developed as a rapid, cost-effective surveillance technique for identifying premises at high risk of breeding A aegypti. The PCI is a simple index incorporating predictors of A aegypti breeding (shade levels, yard tidiness, and house condition) that does not require the inspector to enter the premises. However, weaknesses of the PCI include the inability of the inspector to see into many rear yards and the risk of inconsistent PCI scoring between different health inspectors. The control and prevention of dengue and chikungunya solely depend on vector control methods either through the source reduction evaluated by indices of HI, PCI, and breteau index or through the application of larvicides and insecticides [13,14]. Therefore this study helps to conduct the rapid survey of Aedes species mosquitoes in a place with a minimal number of manpower and in turn, with the help of PCI, delimitation is very possible among villages and hamlets situated in a primary health center area based on the sources of Aedes species breeding habitats [15-17]. It varies based on peoples’ habits, such as water management, water storing habits due to water scarcity, occupation associated with water, like cement works. To employ PCI throughout our state of Tamil Nadu, more experimental studies may be required to make it more suitable.

Acknowledgments

References

• 1. Gubler DJ . Aedes aegypti and Aedes albopictus -borne disease control in the 1990s: top down or bottom up. Am J Trop Med Hyg 6;1989;40(6): 571−8. PMID: 2472746.ArticlePubMed
• 2. World Health Organisation.Diagnosis, prevention and control.. Prentice Hall of India Limited. New Delhi, India, 1998, 2nd edition. pp 48−59.
• 3. Seng CM Jute N . Breeding of Aedes aegypti (L) and Aedes albopictus (Skuse) in urban housing of Sibu town, Sarawak. Southeast Asian J Trop Med Pub Health 9;1994;25(3): 543−8. PMID: 7777923.
• 4. Parker AG Giglioli MEC Mussington S et al. Rock hole habitats of a feral population of Aedes aegypti on the island of Anguilla, West Indies. Mosq News 3;1983;43(1): 79−81.
• 5. Francisco EG Carlosmoisa HS Rafael CC . Factors that modify the larval indices of Aedes aegypti in Colima, Mexico. Rev Panam Salud publica 7;2001;10(1): 6−12. PMID: 11558250.PubMed
• 6. Tun-Lin W Kay BH Barnes A . The premise condition index: a tool for streamlining surveys of domestic Aedes aegypti. Am J Trop Med Hyg 12;1995;53(6): 591−4. PMID: 8561259.ArticlePubMed
• 7. Katyal R Kumar K Gill KS . Breeding of Aedes aegypti and its impact on dengue/ DHF in rural areas of India. Dengue Bull 12;1997;21:1−2.
• 8. Moloney JM Skelly C Weinstein P Maguire M . Domestic Aedes aegypti breeding site surveillance; limitations of remote sensing as a predictive surveillance tool. Am J Trop Med Hyg 8;1998;59(2): 261−4. PMID: 9715943.ArticlePubMed
• 9. Yadava RL Narasimham MVL . Dengue/ dengue hemorrhagic fever and its control in India. Dengue Newsletter 1992;17:3−8.
• 10. Alert CD . Standard case definitions for epidemic prone communicable diseases. Monthly Newsletter of the National Institute of Communicable Diseases, Directorate General of Health Services, Government of India 2001;5(3): 1−8.
• 11. Abdalmagid MA Alhusein Sh H . Etomological investigation of Aedes aegypti in Kassala and Elgadarif states, Sudan. Sudanese J Pub Health 2008;3(2): 77−80.
• 12. Focks DA Chadee DD . Pupal survey: an epidemiologically significant surveillance method for Aedes aegypti an example using data from Trinidad. Am J Trop Med Hyg 2;1997;56:159−67. PMID: 9080874.ArticlePubMed
• 13. Gubler DJ Clark GG . Community-based integrated control of Aedes aegypti: a brief overview of current programs. Am J Trop Med Hyg 1994;50(Suppl): 50−60. PMID: 8024084.ArticlePubMed
• 14. Nathan MB . Critical review of Aedes aegypti control programs in the Caribbean and selected neighboring countries. J Am Mosq Control Assoc 3;1993;9(1): 1−7. PMID: 8468568.PubMed
• 15. Gubler DJ . Dengue and dengue hemorrhagic fever. Clin Microbiol Rev 7;1998;11(3): 480−6. PMID: 9665979.ArticlePubMedPMC
• 16. Goreti Rosa-Freitas M Tsouris P Sibajev A et al. Exploratory temporal and spatial distributions analysis of dengue notifications in Boavista, Roraima, Brazilian, Amazon, 1999–2001. Dengue Bull 2003;27:63−80.PubMed
• 17. Kroeger A Lenhant A Ochoa M et al. Effective control of dengue vectors with curtains and water container covers treated with insecticides in Mexico and Venezuela: cluster randomized trials. BMJ 5;2006;332(7552): 1247−52. PMID: 16735334.Article

Figure & Data

References

Citations

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