Figure 1Effect of Lactobacillus acidophilus on TLR2 mRNA expression in HT29 intestinal epithelial cells. Cells were treated with L. acidophilus and Salmonella enterica serovar Enteritidis (SesE) individually. After 6 hours of incubation, real-time polymerase chain reaction was performed to measure TLR2 mRNA expression levels. Untreated HT29 cells (filled histograms) were used as a control.
Figure 2Effect of Lactobacillus acidophilus on TLR2 mRNA expression in Salmonella enterica serovar Enteritidis (SesE)-infected HT29 cells. Cells were incubated with L. acidophilus for 2 hours and then SesE for 4 hours, or with SesE for 2 hours and then L. acidophilus for 4 hours. At 2 and 4 hours after adding the second bacterium, real-time polymerase chain reaction was performed to measure TLR2 mRNA expression levels. Untreated HT29 cells (filled histograms) were used as a control.
Figure 3Effect of Lactobacillus acidophilus on TLR4 mRNA expression in HT29 cells. Cells were treated with L. acidophilus and Salmonella enterica serovar Enteritidis (SesE) individually. After 6 hours of incubation, real-time polymerase chain reaction was performed to measure TLR4 mRNA expression levels. Untreated HT29 cells (filled histograms) were used as a control.
Figure 4Effect of Lactobacillus acidophilus on TLR4 mRNA expression in Salmonella enterica serovar Enteritidis (SesE)-infected HT29 cells. Cells were incubated with L. acidophilus for 2 hours and then SesE for 4 hours, or with SesE for 2 hours and then L. acidophilus for 4 hours. At 2 and 4 hours after adding the second bacterium, RT-PCR was performed to measure TLR4 mRNA expression levels. Untreated HT29 cells (filled histograms) were used as a control.
Table 1Primers used in the study
Primer |
Sequence (5′ to 3′) |
Reference |
TLR2 F
|
GCA GAA GCG CTG GGG AAT GG |
[17] |
TLR2 R
|
GGA TGC CTA CTG GGT GGA GAA |
[17] |
TLR4 F
|
GGT GGA AGT TGA ACG AAT GG |
[17] |
TLR4 R
|
CCA GCA AGA AGC ATCAGG TG′ |
[17] |
ACTB F
|
CTG GAA GAT GGT GAT GGG AT |
[9] |
ACTB R
|
GGA TTT GGT CGT ATT GGG CG |
[9] |