Objectives
This study aims to develop a high-sensitivity antibody diagnostic kit that will enable a rapid and accurate detection of <i>Cryptospofidium parvum</i> and <i>Giardia lamblia</i> in patients with diarrhea. Methods
The cultivated <i>C. parvum</i> oocysts and <i>G. lamblia</i> cysts in each calf and dog were injected to mice to obtain antibodies, which were titrated. Spleen cells of the immunized mouse were separated and blended with myelomas to produce hybrid cell lines that form monoclonal antibodies. Using ELISA method, antibodies that specifically respond to <i>C. parvum</i> and <i>G.lamblia</i> were then selected. The cells were injected into the abdominal cavity of a BALB/c mouse to isolate hydrops abdominis containing high level of antibodies. The IgG antibody was purified using protein G gel. Results
The detection limit of monoclonal antibodies for <i>Cryptosporidium parvum</i> and <i>Giardia lamblia</i> was 125 oocysts/mL and 1250 cysts/mL, respectively. In addition, during testing they did not show cross-reactivity to viruses (<i>n</i> = 15), bacteria (<i>n</i> =17), and parasites (<i>n</i> = 9). Conclusion
The rapid diagnostic antibody kit developed in this study, which specifically responds to <i>C. parvum</i> and <i>G. lamblia</i>, will be useful in detecting and monitoring diarrheal infections.
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