1. Introduction

In the recent paper in the resistance to fluoroquinolones by the combination of target site mutations and enhanced expression of genes for efflux pumps in Shigella flexneri [1], we presented that MdfA could be related to fluoroquinolone resistance.

The most common example of an major facilitator superfamily (MFS) antibiotic efflux system in gramnegative bacteria is that encoded by the various tet genes associated with tetracycline efflux and resistance [2]. Members of this family of efflux fluoroquinolones are, by contrast, rare in gram-negative bacteria and include only the MdfA transporter of Escherichia coli [3,4]. Therefore, we would like to report the additional evidences that the MdfA is related to fluoroquinolone resistance in S flexneri, even though MdfA does appear to be a more effective pump for nonantibiotics. However, the increased expression level of the MdfA efflux pump mediating fluoroquinolone resistance was first confirmed in the Shigella species strains in this study [1,2]. This study demonstrated that resistance to fluoroquinolone is due to efflux by theMdfA system in the Shigella species. To determine the molecular basis of efflux in the Shigella species, a deletion mutation in mdfA was constructed.

Table 1.

MICs of S flexneri SF021787 and SF021895 isolates

Strains	Substitution in gyrA and parC QRDR		MIC(μg/ml)
	gyrA	parC	CIP	CIP + CCCP	NOR	NOR + CCCP
SF021787	S83L	S80I, R91Q	16	0.125	32	0.25
SF021787ΔmdfA::kan	S83L	S80I, R91Q	12		4
SF021895	S83L, D87G	S80I, R91Q	16	0.125	8	0.25
SF021895ΔmdfA::kan	S83L, D87G	S80I, R91Q	12		4

2. Methods

Deletion of the mdfA gene was performed by the method described by Datsenko and Wanner [5]. The kanamycin resistance gene kan flanked by flippase (FLP) recognition target sites was amplified by a standard polymerase chain reaction (PCR) with the templated plasmid pKD4 and hybrid primers. These primers, P1MdfA (AGCTGCGCTTTATTAAACTCTG CGCGATTA TTATTGGCGAAGAAATTGCGTGTA GGCTGGAGCTGCTTC) and P2MdfA (TCACCATT AATTCGAGAATGCCTGATCGCACAAATCAATCA GGCATTTTTATGGGAATTAGCCATGGTCC), were synthesized with 20 nucleotides (nt) of priming sites 1 and 2 of pKD4 and with 50 nt of the 5' and 3' ends of the mdfA gene. The 1.6 kb PCR fragment was purified and electroporated into S flexneri isolates, 021787 and 021895, into which the red recombinase expression plasmid pKD46 was introduced. Transformants were selected at 37℃ on Luria–Bertani (LB) agar medium containing kanamycin at 50 ug/ml. Homologous recombination between the genomic DNA and the PCR product resulted in the deletion of the mdfA sequence from nt –50 to 1327 (1,377-bp deletion) and its replacement with the kan gene. This was confirmed by two different PCRs. Deletion of mdfA in the transformants was first shown by PCR with primers MdfA3 (GCTGCGCTTTTATTAAACTCTGC) and MdfA4 (CCTGATCGCACAAATCATCA G), whose sequences correspond to sequences flanking the mdfA deletion and that resulted in a 1,227-bp fragment for the parental strains and a negative result when mdfA was deleted and replaced by the kan gene flanked by FLP. The third control PCR, with primers k2 (CGGTGCCCTGAAT GAACTGC) and kt (CGGCCACAGTCGATGAATCC), was used to detect the 471-bp kan fragment.

3. Results and Discussion

The effect of MdfA was confirmed by inactivating the mdfA gene located at different chromosomal loci in the strains studied. The antimicrobial susceptibilities of the parent and mutant strains are presented in Table 1.Both parent strains were resistant to ciprofloxacin (CIP) at MICs of 16 ug/ml and to NOR at MIC of 32 and 8 ug/ml, respectively. SF021787 and SF021895 were resistant to CIP at MICs of 12 ug/ml and to norprofloxacin (NOR) at MICs of 4 ug/ml regardless of the types of GyrA mutations, which suggests that without a functional MdfA. Compared MICs of the parent with △mdfA mutant strains, △mdfA mutant strain was more susceptible to CIP and NOR.

Interestingly, the resistance level to fluoroquinolone in the mutant strains harboring the △mdfA deletion was the same whether the strains carried three or four mutations in gyrA, it because, other efflux system, acrAB and ndeh contributed to resistance against fluoroquinolone.

The low-level changes in MICs of the △mdfA mutant suggest that mdfA contributed to the fluoroquinolone resistance in S flexneri, but it is not likely to be a major effector for higher resistance levels.

Acknowledgements

This study was supported by an intramural grant from the Korea National Institute of Health (2008-N00392-00).

• 1. Kim JY Kim SH Jeon SM et al.. Resistance to fluoroquinolone by the combination of target site mutations and enhanced expression of genes for efflux pumps in Shigella flexneri and Shigella sonnei strains isolated in Korea. Clin Microbiol Infect 8;2008;14(8). 760−5. PubMed
• 2. Poole K . Efflux-mediated multiresistance in Gram-negative bacteria. Clin Microbiol Infect 1;2004;10(1). 12−26. Article
• 3. Edgar R Bibi E . MdfA, an Escherichia coli multidrug resistance protein with an extraordinally broad spectrum of drug recognition. J Bacteriol 4;1997;179(7). 2274−80. ArticlePubMedPMCPDF
• 4. Yang S Clayton SR Zechiedrich EL . Relative contribution of the AcrAB, MdfA, and NorE efflux pumps to quinolone resistance in Escherichia coli. J Animicrobiol Chemother 3;2003;51(3). 545−56.
• 5. Datsenko KA Wanner BL . One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc Natl Acad Sci U S A 6;2000;97(12). 6640−5. ArticlePubMedPMC

Figure & Data

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