Figure 1Effects of control, 50% ethanol extract from Daucus carota roots (100 mg/kg), 50% ethanol extract from Daucus carota roots (200 mg/kg), and ranitidine (50 mg/kg) given orally to the respective groups. Columns for gastric volume (mL/100 g/4 h), pH, and vertical bar represent the mean ± standard error of the mean of six animals. *p < 0.05 against their control. †p < 0.001 against their control. EDC = 50% ethanol extract from Daucus carota roots.
Figure 2Effects of control, 50% ethanol extract from Daucus carota roots (100 mg/kg), 50% ethanol extract from Daucus carota roots (200 mg/kg), and ranitidine (50 mg/kg) given orally to the respective groups. Columns for free acidity (mEq/L/100 g), total acidity (mEq/L/100 g), and vertical bar represent the mean ± standard error of the mean of six animals. *p < 0.05 against their control. †p < 0.001 against their control. EDC = 50% ethanol extract from Daucus carota roots.
Figure 3Effects of control, 50% ethanol extract from Daucus carota roots (100 mg/kg), 50% ethanol extract from Daucus carota roots (200 mg/kg), and ranitidine (50 mg/kg) given orally to the respective groups. Columns for gastric wall mucus (μg/g wet glandular tissue) and vertical bar represent the mean ± standard error of the mean of six animals. *p < 0.05. EDC = 50% ethanol extract from Daucus carota roots.
Figure 4Effects of control, ethanol extract from Daucus carota roots (100 mg/kg), ethanol extract from Daucus carota roots (200 mg/kg), and ranitidine (50 mg/kg) given orally to the respective groups. Columns for glutathione (μg/mg protein) and vertical bar represent the mean ± standard error of the mean of six animals. *p < 0.05. EDC = 50% ethanol extract from Daucus carota roots; GSH = glutathione.
Figure 5Microscopic observation of gastric mucosa of rat stained with hematoxylin and eosin. (A) Pyloric ligation control; (B) 50% ethanol extract from Daucus carota roots (100 mg/kg); (C) 50% ethanol extract from Daucus carota roots (200 mg/kg); and (D) ranitidine (50 mg/kg).
Figure 6Microscopic observation of gastric mucosa of rat stained with hematoxylin and eosin. (A) Ethanol control; lesion in mucosal layer; (B) 50% ethanol extract from Daucus carota roots (100 mg/kg); slight lesion in mucosal layer; (C) 50% ethanol extract from Daucus carota roots (200 mg/kg); and (D) ranitidine (50 mg/kg).
Table 1Effect of 50% ethanol extract from Daucus carota roots on pyloric ligation induced ulceration in rats.
Treatment |
Dose (mg/kg) |
Ulcer index (mean ± SEM)∗
|
% inhibition |
Control |
1 mL |
14.33 ± 1.20 |
– |
EDC |
100 |
5.92 ± 0.45†
|
58.71 |
EDC |
200 |
3.25 ± 0.51†
|
77.32 |
Ranitidine |
50 |
3.08 ± 0.51†
|
78.48 |
Table 2Effect of 50% ethanol extract from Daucus carota roots on ethanol induced ulceration in rats.
Treatment |
Dose (mg/kg) |
Ulcer index (mean ± SEM)∗
|
% inhibition |
Control |
1 mL |
17.00 ± 1.06 |
– |
EDC |
100 |
6.83 ± 0.61†
|
59.80 |
EDC |
200 |
5.17 ± 0.70‡
|
69.61 |
Ranitidine |
50 |
4.83 ± 0.86‡
|
71.57 |
Table 3Level of alanine transaminase (U/L) in different models of ulcer in rats.
Treatment |
Pyloric ligation ulcer∗
|
Alcohol-induced ulcer∗
|
Control |
31.42 ± 2.23 |
32.23 ± 2.32 |
EDC (100 mg/kg) |
23.24 ± 1.64†
|
24.12 ± 4.26†
|
EDC (200 mg/kg) |
22.64 ± 2.15†
|
23.72 ± 2.52†
|