In this study, we measured the drug resistance conferred by <i>mdfA</i> mutations in two <i>Shigella flexneri</i> strains. A mutant in <i>mdfA</i> genes was constructed by polymerase chain reaction–based, one-step inactivation of chromosomal genes. The antimicrobial susceptibility of parent and mutant strains to fluoroquinolones was determined by minimal inhibitory concentration (MICs). The <i>△mdfA</i> mutants were somewhat more susceptible to fluoroquinolones than the parent strains. The low level changes in MICs of the <i>△mdfA</i> mutants suggest that <i>mdfA</i> contributed the fluoroquinolone resistance in <i>S flexneri</i>. This finding found that the increased expression level of an MdfA efflux pump mediated fluoroquinolone resistance, but it is not likely a major effecter of higher resistance levels.
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