Table 1Generation of the recombinant influenza A(H1N1) viruses.
NA mutationa
|
Chungbuk-Y275b
|
Chungbuk-Y275Hc
|
Single mutant |
Double mutant |
Single mutant |
Double mutant |
Triple mutant |
I117V |
Chungbuk-Y275-I117V |
|
Chungbuk-Y275H |
Chungbuk-Y275H-I117V |
|
I117M |
Chungbuk-Y275-I117M |
|
|
Chungbuk-Y275H-I117M |
|
E119V |
Chungbuk-Y275-E119V |
|
|
Chungbuk-Y275H-E119V |
Chungbuk-Y275H-E119V-I117V |
|
|
|
|
|
Chungbuk-Y275H-E119V-I117M |
I223V |
Chungbuk-Y275-I223V |
|
|
Chungbuk-Y275H-I223V |
|
Y275H |
|
|
|
|
|
R293K |
Chungbuk-Y275-R293K |
|
|
Chungbuk-Y275H-R293K |
|
N295S |
Chungbuk-Y275-N295S |
Chungbuk-Y275-N295S-I117V |
|
Chungbuk-Y275H-N295S |
Chungbuk-Y275H-N295S-I117V |
|
|
Chungbuk-Y275-N295S-I117M |
|
|
Chungbuk-Y275H-N295S-I117M |
|
|
Chungbuk-Y275-N295S-I223V |
|
|
Chungbuk-Y275H-N295S-I223V |
S334N |
Chungbuk-Y275-S334N |
|
|
Chungbuk-Y275H-S334N |
|
Table 2Susceptibility of the recombinant influenza A(H1N1) viruses to NAIs.
Recombinant virus |
Neuraminidase inhibition assay
|
Oseltamivir
|
Zanamivir
|
Mean IC50 ± SD (nM)a
|
Fold changeb
|
Mean IC50 ± SD (nM)a
|
Fold changeb
|
Chungbuk-Y275H |
1.1 ± 0.5 |
1.0 |
2.0 ± 0.6 |
1.0 |
Chungbuk-Y275H-I117V |
2.0 ± 0.4 |
1.8 |
1.1 ± 0.4 |
0.5 |
Chungbuk-Y275H-I117M |
0.4 ± 0.1 |
0.4 |
0.4 ± 0.1 |
0.2 |
Chungbuk-Y275H-E119V |
16.9 ± 2.0 |
15.4 |
271.5 ± 17.7 |
135.7 |
Chungbuk-Y275H-I223V |
1.9 ± 0.2 |
1.7 |
0.6 ± 0.4 |
0.3 |
Chungbuk-Y275H-R293K |
0.6 ± 0.1 |
0.5 |
35.7 ± 6.3 |
17.8 |
Chungbuk-Y275H-N295S |
44.3 ± 7.0 |
40.2 |
2.2 ± 1.0 |
1.1 |
Chungbuk-Y275H-S334N |
0.7 ± 0.1 |
0.6 |
0.4 ± 0.1 |
0.2 |
Chungbuk-Y275H-E119V-I117V |
7.4 ± 0.4 |
6.7 |
781.3 ± 39.5 |
390.6 |
Chungbuk-Y275H-E119V-I117M |
21.5 ± 2.2 |
19.5 |
362.0 ± 22.0 |
181.0 |
Chungbuk-Y275H-N295S-I117V |
66.1 ± 13.5 |
60.0 |
4.0 ± 1.0 |
2.0 |
Chungbuk-Y275H-N295S-I117M |
57.7 ± 17.2 |
52.4 |
7.5 ± 2.2 |
3.7 |
Chungbuk-Y275H-N295S-I223V |
107.2 ± 8.3 |
97.4 |
3.1 ± 0.4 |
1.5 |
Chungbuk-Y275 |
552.4 ± 67.6 |
1.0 |
1.3 ± 0.9 |
1.0 |
Chungbuk-Y275-I117V |
751.8 ± 51.1 |
1.3 |
1.0 ± 0.4 |
0.7 |
Chungbuk-Y275-I117M |
434.8 ± 12.6 |
0.7 |
0.8 ± 0.2 |
0.6 |
Chungbuk-Y275-E119V |
23.4 ± 2.4 |
0.04 |
263.3 ± 47.3 |
202.5 |
Chungbuk-Y275-I223V |
642.8 ± 24.0 |
1.1 |
0.7 ± 0.2 |
0.5 |
Chungbuk-Y275-R293K |
557.5 ± 12.8 |
1.0 |
2.2 ± 0.4 |
1.6 |
Chungbuk-Y275-N295S |
118.9 ± 20.0 |
0.2 |
2.4 ± 0.4 |
1.8 |
Chungbuk-Y275-S334N |
456.7 ± 38.2 |
0.8 |
0.6 ± 0.2 |
0.4 |
Chungbuk-Y275-N295S-I117V |
369.6 ± 40.5 |
0.6 |
34.8 ± 10.8 |
26.7 |
Chungbuk-Y275-N295S-I117M |
133.2 ± 12.5 |
0.2 |
21.3 ± 5.0 |
16.3 |
Chungbuk-Y275-N295S-I223V |
330.4 ± 55.1 |
0.5 |
26.3 ± 7.7 |
20.2 |